Abstract:
:The aim of this study was to investigate the morphologic and ultrastructural features of biofilms of slow and fast-growing mycobacteria in different stress conditions, presence and absence of oleic acid albumin dextrose catalase (OADC) enrichment and at different temperatures: 30, 37 and 42 °C. Four hundred mycobacterial isolates were taken. The biomass of each biofilm was quantified using a modified microtiter plate assay method. Isolates were divided into those that formed fully established biofilms, moderately attached biofilms and weakly adherent biofilms by comparison with a known biofilm-forming strain. The large quantity of biofilm was produced by Mycobacterium smegmatis at temperature 37 and 42 °C as compared to 30 °C. Mycobacterium fortuitum and M. avium developed large amount of biofilm at 30 °C as compared to 37 and 42 °C. Mycobacterium tuberculosis developed strong biofilm at 37 °C and no biofilm at 30 and 42 °C in Sauton's media. The selected non-tuberculous mycobacteria and H37Rv developed strong biofilm in the presence of OADC enrichment in Sauton's medium. Microscopic examination of biofilms by scanning electron microscopy revealed that poorly adherent biofilm formers failed to colonize the entire surface of the microtiter well. While moderately adherent biofilm formers grew in uniform monolayers but failed to develop a mature three-dimensional structure. SEM analysis of an isolate representative of the group formed fully established biofilms with a textured, multi-layered, three-dimensional structure.
journal_name
Ultrastruct Patholjournal_title
Ultrastructural pathologyauthors
Kumar V,Sachan TK,Sharma P,Rawat KDdoi
10.3109/01913123.2014.950780subject
Has Abstractpub_date
2015-02-01 00:00:00pages
38-48issue
1eissn
0191-3123issn
1521-0758journal_volume
39pub_type
杂志文章abstract::The authors present the case of a young man with aplastic anemia who went into shock and died after several red blood cell unit transfusions. Immunohematological studies did not show any abnormality and blood cultures from patients and blood bags were negative. The ultrastructural findings, allied with current scienti...
journal_title:Ultrastructural pathology
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doi:10.1080/01913120902785567
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abstract::Morphologic evidence of intercellular junctions was found between polymorphonuclear leukocytes (PMNs) and between PMNs and tumor cells. All these junctions displayed electron-dense plaques along the plasma membranes with linear spaces in between. These plaques were finely granular and devoid of filaments. Fine granule...
journal_title:Ultrastructural pathology
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doi:10.3109/01913128309141835
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abstract::Electron microscopy of the central nervous system surface structure is described in two fetuses with Fukuyama congenital muscular dystrophy (FCMD). In addition to relatively large surface defects, many minute defects less than several micrometers in size associated with protrusion of glial cytoplasm were observed in t...
journal_title:Ultrastructural pathology
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doi:10.3109/01913129709021933
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abstract::Finger pulp biopsies of 31 patients suffering from Raynaud's syndrome (RS) were investigated by light and electron microscopy. The patients suffered from various underlying diseases. Interest was focused on the vascular changes and nerve and connective tissue alterations, especially the elastic fiber changes. In the d...
journal_title:Ultrastructural pathology
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doi:10.3109/01913128209016642
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abstract::Strabismus is an ocular disorder characterized by partial or complete inability to keep eye alignment. It represents a very common ocular problem at ophthalmology clinics worldwide. The current study aimed to show the most encountered ultrastructural changes in extraocular muscles (EOMs) collected from patients with d...
journal_title:Ultrastructural pathology
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abstract::Primary cilia are hair-like organelles singly distributed along the apical surface of proximal and distal nephron tubules as mechanosensors. The goal of this study was to use electron microscopy to systemically evaluate cilia changes in acute tubular injury (ATI) from both transplant and native renal biopsies. Three g...
journal_title:Ultrastructural pathology
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abstract::The aim of this work was the study of ultrastructural morphologic and morphometric changes in myocardiocytes from chronic chagasic hamsters (Mesocricetus auratus), comparatively with changes described in other animal models and in human Chagas disease. Four chagasic animals and three nonchagasic controls were studied....
journal_title:Ultrastructural pathology
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journal_title:Ultrastructural pathology
pub_type: 杂志文章
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更新日期:1998-09-01 00:00:00
abstract::This report ultrastructurally illustrates the giant lysosome-related organelles in the various cellular components of a renal biopsy from a 10 year-old female affected by the Chédiak-Higashi syndrome. Albeit similar observations have been made and reported in animal models of the syndrome, to the author's knowledge, t...
journal_title:Ultrastructural pathology
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doi:10.3109/01913123.2011.584656
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abstract::Six malignant schwannomas were studied by electron microscopy and immunohistochemistry for S-100 protein at the light and electron microscopic levels to clarify the nature of the tumor cells. Three tumors (group A) were composed of poorly differentiated tumor cells and showed no immunoreactivity for S-100 protein. One...
journal_title:Ultrastructural pathology
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更新日期:1988-01-01 00:00:00
abstract::To investigate the effects of X-ray irradiation on the proliferation and apoptosis of MCF-7 breast cancer cells; MCF-7 breast cancer cells were irradiated with X-ray. After irradiation, morphological changes and growth inhibition rate of the irradiated cells were observed under an inverted microscope. 3-(4,5-dimethylt...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913123.2013.861569
更新日期:2014-05-01 00:00:00
abstract::The objective of this study was to investigate the ultrastructural characteristics of hybrid acute leukemia (HAL). Fifteen cases of HAL were studied by transmission electron microscopy (TEM), focusing on organelles and myeloperoxidase (MPO) reaction of leukemic cells. By TEM, 5 out 15 cases of HAL were consistent with...
journal_title:Ultrastructural pathology
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更新日期:2005-09-01 00:00:00
abstract::Cellular (c) fibronectins (Fn) differ biochemically, immunologically, and functionally from plasma fibronectins (pFn). Most existing data on Fn distribution in the normal and diseased liver require revision because those studies were based on reagents that did not distinguish pFn from cFn and predated the development ...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129509014601
更新日期:1995-01-01 00:00:00
abstract::Inflammation in the liver is an extraintestinal manifestation that is frequently seen during inflammatory bowel diseases (IBD). The authors investigated histopathologycal, ultrastructural and antioxidant effects of dexmedetomidine (Dex) on liver during trinitrobenzene sulfonic acid (TNBS)-induced inflammatory bowel di...
journal_title:Ultrastructural pathology
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doi:10.3109/01913123.2013.829150
更新日期:2015-02-01 00:00:00
abstract::Pleomorphic liposarcoma (PL) is the least common subtype of liposarcoma, displaying a lipoblastic, malignant fibrous histiocytoma (MFH)-like and, less frequently, an epithelioid growth pattern. The epithelioid morphology in PL is still underrecognized and may closely simulate other epithelial neoplasms, mainly adrenal...
journal_title:Ultrastructural pathology
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doi:10.1080/01913120290104575
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abstract::A spectrum of 24 benign and malignant nerve sheath tumors and 10 non-neural spindle-cell tumors were studied by light microscopy for the presence of glial fibrillary acidic protein (GFAP) immunoreactivity by the peroxidase-antiperoxidase (PAP) technique. In 8 cases, these results were compared to their electron micros...
journal_title:Ultrastructural pathology
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doi:10.3109/01913128409141487
更新日期:1984-01-01 00:00:00
abstract::Peeling skin syndrome is a rare dermatosis characterized by spontaneous and painless peeling of the skin. The authors report two patients with history of spontaneous, asymptomatic, and noninflammatory peeling skin of the acral surfaces after soaking in water. On light microscopy, blisters were located in the mid layer...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/01913120590909867
更新日期:2005-01-01 00:00:00
abstract::An astroblastoma of high-grade type arising in the brain of a 3-year-old child is reported. The first description of the ultrastructural, immunohistochemical, and cytogenetic findings in this rare tumor variant are presented. ...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/019131299281473
更新日期:1999-09-01 00:00:00
abstract::To evaluate the contribution of electron microscopy to the final diagnosis of glomerulopathies, the authors established a prospective study during the first semester of 2006. A total of 52 kidney biopsies were performed with 3 samples for light microscopy, immunofluorescence, and electron microscopy. Among these renal...
journal_title:Ultrastructural pathology
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更新日期:2010-04-01 00:00:00
abstract::Our previous histochemical and ultrastructural studies have identified, in human catecholamine neurons, abundant spherical acidophilic protein bodies (pb), which originate from regular mitochondria, retaining their double membrane. In locus coeruleus (LC) neurons, pb have somatodendritic distribution and are unequivoc...
journal_title:Ultrastructural pathology
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更新日期:2013-04-01 00:00:00
abstract::Three cases of adamantinomatous craniopharyngiomas were examined by light and electron microscopy and special attention was paid to the formation of ossified tissue. The tumors were composed of neoplastic epithelial cells with keratinized cell nests and fibrous connective tissue. Keratinized cell masses sometimes dire...
journal_title:Ultrastructural pathology
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journal_title:Ultrastructural pathology
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abstract::Disseminated dermal tumors in a 71-year-old male represented the first clinical manifestation of a chronic myelomonocytic leukemia. The dermal infiltrate in one of the nodules consisted predominantly of closely packed pleomorphic Langerhans' cells (LC) with typical Birbeck granules and a strong reactivity for S-100 pr...
journal_title:Ultrastructural pathology
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更新日期:1988-03-01 00:00:00
abstract::The ultrastructural appearances of corneal crystalloidal deposits are described in two patients with an IgG-kappa paraproteinemia of uncertain pathogenesis. The crystalloids in one patient were overwhelmingly intracellular and were found mainly in stromal keratocytes, but also in basal corneal epithelial cells and the...
journal_title:Ultrastructural pathology
pub_type: 杂志文章,评审
doi:10.3109/01913129309027800
更新日期:1993-11-01 00:00:00
abstract::As the name suggests, the histogenesis of the granular cell tumor is uncertain, but this intriguing neoplasm has occasioned much speculation and controversy since its description in 1926 as a myoblastoma. Though ubiquitous in its anatomic location, it is usually small, solitary, benign, and readily recognized by routi...
journal_title:Ultrastructural pathology
pub_type: 杂志文章,评审
doi:10.1080/019131299281545
更新日期:1999-07-01 00:00:00
abstract::The present study provides ultrastructural information about the acaricidal effects of neem (Azadirachta indica A. Juss) on the ovaries of R. sanguineus engorged females. In general, the main damage caused in the oocytes was alteration in the shape of the cell and of the germinal vesicle, ring-shaped nucleolus, cytopl...
journal_title:Ultrastructural pathology
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更新日期:2012-02-01 00:00:00
abstract::Microvascular injury is an important factor in renal allograft survival. Repeated episodes of endothelial injury from chronic antibody-mediated rejection typically manifest at the ultrastructural level as circumferential multilayering of remodeled glomerular basement membrane material and peritubular capillary basal l...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
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更新日期:2018-07-01 00:00:00
abstract::A fast method for processing biologic material for electron microscopy for precise and specific diagnosis of infectious agents is an increasing necessity. After different, reportedly fast methods were tested, a useful and quick technique was developed that provides well-preserved cellular structures, enabling the etio...
journal_title:Ultrastructural pathology
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更新日期:1992-07-01 00:00:00
abstract::Pathologic changes induced in the small intestine of suckling mice by rotavirus infection were studied by conventional histology, immunofluorescence, scanning electron microscopy, and electron microscopy of ultrathin sections. Infection could be detected within 24 hours in a few mice, but after 2 days it was well esta...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128109031504
更新日期:1981-01-01 00:00:00
abstract::Glycogen branching enzyme deficiency/Andersen disease can manifest with a spectrum of clinical phenotypes, making the diagnosis difficult. An 11-year-old Pakistani boy presented with a history of progressive weakness and delayed milestones. Echocardiography showed features of dilated cardiomyopathy. He was suspected t...
journal_title:Ultrastructural pathology
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更新日期:2015-01-01 00:00:00