Abstract:
:Automation of AST has come quite some way and is here to stay. In particular, fully automated, "hands off" instruments have great appeal to laboratories with a limited number of well-trained and experienced clinical microbiology personnel. None of the evaluated instruments is perfect, but then neither are the standard or reference techniques. Overnight incubation has been the yardstick since the early days of in vitro AST. Given the usually shorter therapeutic intervals of 4- to 12-hour dosage schedules, it is quite possible that shorter incubation times for in vitro tests will become more of a standard. Until that time, newer, including automatic, techniques need to be evaluated against the more traditional standard methods. Quality control is critical, and since no systematic approach aside from individual manufacturers' suggestions exists, it should be developed by the NCCLS or similar agencies. Quality control might include standards for the evaluation of such equipment and systems because the development of new technology in this area will continue. Overall, reproducibility and accuracy of the instruments and methods evaluated were quite promising and should encourage well-designed studies of clinical correlation and relevance. The AMS equipment has been in use for routine AST in the clinical laboratories of the Seattle Veterans Administration Medical Center and the University of Washington Hospital. Because of its simplicity and flexibility, the Kirby-Bauer method continues to be an alternate technique for certain important clinical isolates, for instance, blood cultures in both laboratories. Finally, it should be remembered that the most critical function of all such equipment is the reliable detection of resistance.
journal_name
Clin Lab Medjournal_title
Clinics in laboratory medicineauthors
Kiehlbauch J,Kendle JM,Carlson LG,Schoenknecht FD,Plorde JJsubject
Has Abstractpub_date
1989-06-01 00:00:00pages
319-40issue
2eissn
0272-2712issn
1557-9832journal_volume
9pub_type
杂志文章,评审abstract::Image processing has traveled to the lunar surface and back, finding its way into the clinical laboratory. Advances in digital computers have improved the technology of image analysis, resulting in a wide variety of medical applications. Offering improvements in turnaround time, standardized systems, increased precisi...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1988-12-01 00:00:00
abstract::Diagnosis of invasive fungal pneumonias by conventional culture methods is difficult to assess and often delayed. Nonmolecular fungal markers have emerged as an important adjunctive tool to support their diagnosis in combination with other clinical, radiologic, and microbiological criteria of invasive fungal diseases....
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2014.02.006
更新日期:2014-06-01 00:00:00
abstract::"Bath salts" has attracted young adults primarily due to its stimulatory and hallucinogenic effects akin to amphetamines and cocaine. Although other designer amines have been incorporated to newer generation "bath salts", synthetic cathinones remain to be their major component. This article discusses our current under...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2012.07.010
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abstract::When one looks at the field of molecular pathology or transplantation, it is evident that molecular biology has made a positive impact on medicine. However, the progress in transfusion medicine has been slower and more cautious than in other areas of the clinical laboratory. To understand where the field may go in the...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章
doi:10.1016/j.cll.2010.02.004
更新日期:2010-06-01 00:00:00
abstract::Diseases due to nontuberculous mycobacteria are increasing in frequency, especially in patients with compromised immunity. A number of "new" mycobacterial species have been described in the last decade, largely as the result of the use of new tools to identify previously unrecognized mycobacteria found in the environm...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2004.05.011
更新日期:2004-09-01 00:00:00
abstract::The last decade has witnessed the transformation of first-trimester Down syndrome screening from an aspiration to a clinical reality. First-trimester screening now equals or exceeds the diagnostic accuracy of conventional midtrimester screen while realizing an important desire of pregnant women for early diagnosis. Be...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2010.05.001
更新日期:2010-09-01 00:00:00
abstract::Extracellular vesicles (EVs) are membranous particles found in a variety of biofluids that encapsulate molecular information from the cell, which they originate from. This rich source of information that is easily obtained can then be mined to find diagnostic biomarkers. This article explores the current biological un...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2016.05.004
更新日期:2016-09-01 00:00:00
abstract::Routine biochemical tests generally include serum enzymes, proteins, and other markers useful for identifying hepatobiliary disease in dogs and cats. Obtaining results outside the reference intervals can occur with direct hepatocellular injury, enzyme induction by hepatocytes or biliary epithelium, or decreased hepati...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2015.05.002
更新日期:2015-09-01 00:00:00
abstract::The ability to identify specific strains within a given species of pathogen is an important aid in the rational development of effective measures to prevent and control nosocomial infections. The efforts of both microbiologist and hospital epidemiologic are facilitated greatly by the availability of the newer molecula...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1995-06-01 00:00:00
abstract::There are multiple levels of transcriptional regulation that a cell can bring to bear on either native cellular genes or integrated proviral genes. One such level of regulation is inducible sequence-specific transcriptional factors, such as NF-kappa B, which have been extensively investigated with respect to the HIV-1...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1994-06-01 00:00:00
abstract::The subject of this article is privacy and its relationship to the activities of health care organizations. Privacy is a good quality that each individual may possess. The quality of an individual's privacy is a personal determination. Although everyday life requires that we attempt to understand and protect the priva...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::Concern for illicit and restricted drug use in otolaryngology is similar to other surgical specialties with a few notable exceptions. Many illicit drugs are consumed transnasally. Repeated nasal exposure to stimulants or narcotics can cause local tissue destruction that can present as chronic rhinosinusitis or nasosep...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2016.07.012
更新日期:2016-12-01 00:00:00
abstract::The primary product of the laboratory is information. Greater attention should be devoted to how laboratory reports are presented to ensure that information is being conveyed effectively to the clinician. Different reporting techniques, including test-by-test, interim, cumulative, and completed accession reports, are ...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1991-03-01 00:00:00
abstract::The pathologist, by virtue of his or her observations and documentation of injuries with concomitant attempts to discern patterns of injury and correlate autopsy findings with investigative details, will often play an integral and primary role in the death investigation process. The information gleaned during this pro...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1998-06-01 00:00:00
abstract::Considerable advances have been made in identifying women whose pregnancies are at the greatest risk for fetal Down syndrome and other aneuploidies. Maternal serum tests and ultrasonography in either the first or second trimester provide effective prenatal screening. However, the most efficacious protocols are based o...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2010.04.013
更新日期:2010-09-01 00:00:00
abstract::ALP is a profoundly ubiquitous enzyme that originates in many, if not all, mammalian tissues. The development of a simple ISEF technique has allowed reflection of at least 12 cellular components. Improvements in technique may add further zones of information. The aura of darkness surrounding ALP is being slowly dissip...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1989-12-01 00:00:00
abstract::The interpretation of the total serum testosterone concentration is problematic because it is related directly to the serum SHBG concentration.Frequently, an estimate of the serum free testosterone concentration is obtained to better assess the clinical status of the patient. We reviewed five methods for the determina...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2004.01.010
更新日期:2004-03-01 00:00:00
abstract::It is necessary to determine the safety of polymeric materials through laboratory and animal testing before biomedical use can become more widespread. This article describes many of the methods used in analyzing the toxicology and biocompatibility of potentially important biomedical polymers. The study method used in ...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1990-09-01 00:00:00
abstract::All clinical laboratory testing in the United States is regulated by the Clinical Laboratory Improvement Amendments of 1988 (CLIA'88 or CLIA) and overseen by the Centers for Medicare and Medicaid Services. CLIA profoundly changed the prevailing United States regulatory philosophy by imposing uniform requirements for a...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章
doi:10.1016/j.cll.2009.06.012
更新日期:2009-09-01 00:00:00
abstract::In discussing quality assurance in the blood bank, where many of the standard statistical techniques of measuring accuracy simply do not apply, three questions come to mind: How much? How often? Who says? Because of severe changes in the prospective payment plan and a new interest in cost effectiveness, many transfusi...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1986-12-01 00:00:00
abstract::Objective evidence-based national surveys serve as a first step in identifying suitable point-of-care device designs, effective test clusters, and environmental operating conditions. Preliminary survey results show the need for point-of-care testing (POCT) devices using test clusters that specifically detect pathogens...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章
doi:10.1016/j.cll.2009.07.014
更新日期:2009-09-01 00:00:00
abstract::Disease prevention requires sociopolitical change, which in turn requires the participation of those for whom the change is intended, including demographic groups at high risk for disease, appropriate governmental authorities, and essential health care personnel. Multiparous women of lowest socioeconomic status, the d...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2004.05.018
更新日期:2004-12-01 00:00:00
abstract::Transfusion medicine is a rapidly evolving specialty, and efforts are ongoing to improve the safety and quality of blood component therapy. Leukocytes are known to be the cause of numerous adverse effects of transfusion therapy, and their removal from red cells and platelet components may be desirable in a variety of ...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1992-12-01 00:00:00
abstract::Pet guinea pigs are presented to veterinary clinics for routine care and treatment of clinical diseases. In addition to obtaining clinical history and exam findings, diagnostic testing may be required, including hematological assessments. This article describes common blood collection methods, including venipuncture s...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2015.05.012
更新日期:2015-09-01 00:00:00
abstract::To leverage the time and expertise of the pathologist, a comprehensive workstation is a useful tool. Because the practice of pathology is so diverse, however, there is no one standard for what constitutes a workstation. The author reviews the several elements and components which must be included (access to your LIS, ...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1997-06-01 00:00:00
abstract::Cytomorphological examination of aspirate smears remains the basic method to diagnose hematologic disorders and to evaluate treatment-related changes. Last-generation hematological analyzers can count, besides cells normally circulating in peripheral blood, some types of immature and abnormal cells, such as erythrobla...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2014.10.001
更新日期:2015-03-01 00:00:00
abstract::An exciting era exists as a result of the introduction of novel markers of myocardial damage. The prognostic implications of troponins, even in the setting of "minor myocardial damage," are potentially of immense value to patient care. We need to take the next step and assess the outcomes of interventions in these set...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1997-12-01 00:00:00
abstract::Differentiation between procedure-related necrosis and postprocedural myocardial infarction (MI) is challenging because of the inherent association of these procedures to varying levels of myocardial injury. To improve risk stratification of patients at risk of an acute MI, the universal definition of MI implemented c...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2013.11.013
更新日期:2014-03-01 00:00:00
abstract::This article focuses on current clinical laboratory testing to diagnose Alzheimer disease and monitor its progression throughout its disease course. Several clinically available tests focus on analysis of amyloid and tau levels in cerebrospinal fluid as well as autosomal dominant and risk factor genes. Although the cu...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:10.1016/j.cll.2020.05.003
更新日期:2020-09-01 00:00:00
abstract::Processing of specimens for mycobacteria need to proceed as rapidly as possible to provide 24 hour turnaround for smears and 21-day turnaround for the identification of Mycobacterium tuberculosis. If these goals are to be accomplished, a broth culture or microcolony plate method must be part of the media and in the pl...
journal_title:Clinics in laboratory medicine
pub_type: 杂志文章,评审
doi:
更新日期:1996-09-01 00:00:00