Abstract:
BACKGROUND:Microsatellite instability (MSI) is a useful phenotype in cancer diagnosis and prognosis. Nevertheless, methods to detect MSI status from next generation DNA sequencing (NGS) data are underdeveloped. METHODS:We developed an approach to detect the MSI phenotype using NGS (mSINGS). The method was used to evaluate mononucleotide microsatellite loci that were incidentally sequenced after targeted gene enrichment and could be applied to gene or exome capture panels designed for other purposes. For each microsatellite locus, the number of differently sized repeats in experimental samples were quantified and compared to a population of normal controls. Loci were considered unstable if the experimental number of repeats was statistically greater than in the control population. MSI status was determined by the fraction of unstable microsatellite loci. RESULTS:We examined data from 324 samples generated using targeted gene capture assays of 3 different sizes, ranging from a 0.85-Mb to a 44-Mb exome design and incorporating from 15 to 2957 microsatellite markers. When we compared mSING results to MSI-PCR as a gold standard for 108 cases, we found the approach to be both diagnostically sensitive (range of 96.4% to 100% across 3 panels) and specific (range of 97.2% to 100%) for determining MSI status. The fraction of unstable microsatellite markers calculated from sequencing data correlated with the number of unstable loci detected by conventional MSI-PCR testing. CONCLUSIONS:NGS data can enable highly accurate detection of MSI, even from limited capture designs. This novel approach offers several advantages over existing PCR-based methods.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Salipante SJ,Scroggins SM,Hampel HL,Turner EH,Pritchard CCdoi
10.1373/clinchem.2014.223677subject
Has Abstractpub_date
2014-09-01 00:00:00pages
1192-9issue
9eissn
0009-9147issn
1530-8561pii
clinchem.2014.223677journal_volume
60pub_type
杂志文章abstract::Laboratories of the Lipid Research Clinics Program (LRC) maintained the accuracy of their measurements of total cholesterol by using seven pooled serum calibrators supplied by the Centers for Disease Control (CDC). Over the 11-year life of the LRC, each calibrator was prepared in succession and a target value was assi...
journal_title:Clinical chemistry
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doi:
更新日期:1986-04-01 00:00:00
abstract::Liquid scintillation counting of 3H-labeled whole-blood samples is severely impaired owing to quenching by blood pigments. In this study, dry oxidation and chemical solubilization followed by decolorization were the two general methods used to eliminate color quenching. Three blood volumes were examined: 0.25, 0.50, a...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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更新日期:1993-02-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1992-01-01 00:00:00
abstract::We describe a system for detection of leukemia cells involving complement-mediated cytotoxic reaction and an image processing system, consisting of a charge-coupled-device image sensor, an image memory board, a personal computer, and a phase-contrast microscope. Then added to a cell suspension, monoclonal antibody spe...
journal_title:Clinical chemistry
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doi:
更新日期:1987-04-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1990-07-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1989-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1983-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 共识发展会议,杂志文章,评审
doi:
更新日期:1990-08-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1986-01-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1984-03-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1991-08-01 00:00:00
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doi:
更新日期:1992-09-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1984-06-01 00:00:00
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doi:
更新日期:1990-03-01 00:00:00
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doi:
更新日期:1975-09-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1981-09-01 00:00:00
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doi:
更新日期:1988-03-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1992-06-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1978-03-01 00:00:00
abstract::Since 1991, the Ontario Laboratory Proficiency Testing Program has assessed the analytical performance of creatine kinase (CK; EC 2.7.3.2) isoenzyme-2, using fresh human serum supplemented with purified human CK isoenzymes. In Ontario, the 142 laboratories licensed to analyze CK-2 use a variety of methods: electrophor...
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doi:
更新日期:1994-02-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1982-04-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1979-04-01 00:00:00
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journal_title:Clinical chemistry
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