Engineered minichromosomes in plants.

Abstract:

:Platforms for the development of synthetic chromosomes in plants have been produced in several species using telomere mediated chromosomal truncation with the simultaneous inclusion of sites that facilitate further additions to the newly generated minichromosome. By utilizing truncated supernumerary or B chromosomes, the output of the genes on the minichromosome can be amplified. Proof of concept experiments have been successful illustrating that minichromosome platforms can be modified in vivo. Engineered minichromosomes can likely be combined with haploid breeding if they are incorporated into inducer lines given that the observations that basically inert chromosomes from haploid inducer lines can be recovered at workable frequencies in otherwise haploid plants. Future needs of synthetic chromosome development are discussed.

journal_name

Curr Opin Plant Biol

authors

Birchler JA

doi

10.1016/j.pbi.2014.05.009

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

76-80

eissn

1369-5266

issn

1879-0356

pii

S1369-5266(14)00065-X

journal_volume

19

pub_type

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