Abstract:
:The bioassay system from American Diagnostic for amikacin was compared to the same company's radioimmunoassay (RIA) technique used in this hospital. The bioassay is performed by adding serum from a patient to wells cut in agar which have been seeded with a susceptible fast growing microorganism (Enterobacter). After incubation of five hours at 37 degrees, the zones of inhibition for four standards are measured and plotted. The amount of amikacin in the serum sample is then determined from the linear graph. Ten replicates of a serum with 8 microgram per ml of amikacin and 10 replicates of a serum with 20 microgram per ml of amikacin were assayed on each of four days. Aliquots of the same samples were assayed in a similar manner by RIA. The coefficients of variation (CVs) of the within run assays by the bioassay and RIA methods for the low amikacin test sample ranged from 4.7 to 7.2 percent and 6.2 to 13.0 percent, respectively. The within run CVs for the high amikacin test sample for the bioassay and RIA methods ranged from 5.2 to 6.9 percent and 5.0 to 13.9 percent, respectively. The day to day and overall CVs for the bioassay and RIA methods for the low amikacin test sample were 1.5 and 5.8 percent and 7.4 and 11.8 percent, respectively. For the high amikacin test sample, they were 0.4 and 6.0 percent and 4.0 and 9.6 percent, respectively. The correlation coefficient for 49 sera from patients on amikacin was 94 percent between the two methods.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Manos JP,Botting AJ,Burdash NM,Mason MH,West MEsubject
Has Abstractpub_date
1981-09-01 00:00:00pages
411-5issue
5eissn
0091-7370issn
1550-8080journal_volume
11pub_type
杂志文章abstract::The existence of clonorchiasis cases among the Asian immigrants and the major clinical and pathologic features encountered in those patients is emphasized. Owing to the longevity of the parasite, clinical symptoms may occur long after endemic exposure. The common manifestations among the immigrants are recurrent pyoge...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
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更新日期:1984-05-01 00:00:00
abstract::A variety of vaginal and cervical abnormalities have been encountered in the offspring of women who have taken stilbestrol or chemically related nonsteroidal estrogens during pregnancy. Cervical erosion has been noted most often, but vaginal adenosis has been proven by biopsy in over 30 percent, and transverse vaginal...
journal_title:Annals of clinical and laboratory science
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更新日期:1974-07-01 00:00:00
abstract::Blood lead levels were measured by atomic absorption spectrophotometry in 86 San Diego policemen to see if they would reflect the known high atmospheric lead levels in San Diego. The mean blood lead for the entire group was 27.0 microgram per dl, a level very similar to that found in the general population of other ci...
journal_title:Annals of clinical and laboratory science
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更新日期:1977-07-01 00:00:00
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journal_title:Annals of clinical and laboratory science
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abstract:BACKGROUND:Long-term use of hormones and immunosuppressive agents is necessary for treating polymyositis (PM)/dermatomyositis (DM) but may cause liver damage. At what point do the costs of treatment outweigh the benefits, and how will the breakeven point be determined? METHODS:Serum muscle enzyme changes in 93 PM/DM p...
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journal_title:Annals of clinical and laboratory science
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journal_title:Annals of clinical and laboratory science
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更新日期:1987-05-01 00:00:00
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journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
doi:
更新日期:1980-05-01 00:00:00
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journal_title:Annals of clinical and laboratory science
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journal_title:Annals of clinical and laboratory science
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更新日期:2012-10-01 00:00:00
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journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
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journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
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更新日期:1984-03-01 00:00:00
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journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
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journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
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更新日期:1980-11-01 00:00:00
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journal_title:Annals of clinical and laboratory science
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journal_title:Annals of clinical and laboratory science
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journal_title:Annals of clinical and laboratory science
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更新日期:2015-10-01 00:00:00
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journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章,评审
doi:
更新日期:1981-07-01 00:00:00
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journal_title:Annals of clinical and laboratory science
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journal_title:Annals of clinical and laboratory science
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journal_title:Annals of clinical and laboratory science
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journal_title:Annals of clinical and laboratory science
pub_type: 临床试验,杂志文章,随机对照试验
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abstract::BCR/ABL1 transcripts, the molecular hallmarks of chronic myeloid leukemia (CML), have been detected in peripheral blood from healthy individuals. Although CML is a sporadic disease, familial occurrence has been reported. This raises the question of whether there is a hereditary factor related to the etiology of CML. O...
journal_title:Annals of clinical and laboratory science
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更新日期:2019-11-01 00:00:00
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journal_title:Annals of clinical and laboratory science
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更新日期:1980-11-01 00:00:00
abstract::Trace element status was ascertained in 19 alcoholic patients under long-term treatment with disulfiram and in 12 alcoholic patients treated for the first time; the latter group was re-examined after four weeks of treatment. Both groups were compared to matched controls with a moderate alcohol intake. The copper/zinc ...
journal_title:Annals of clinical and laboratory science
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00
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journal_title:Annals of clinical and laboratory science
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更新日期:2004-07-01 00:00:00
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journal_title:Annals of clinical and laboratory science
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