Simultaneous determination of calycosin-7-O-β-D-glucoside, ononin, calycosin, formononetin, astragaloside IV, and astragaloside II in rat plasma after oral administration of Radix Astragali extraction for their pharmacokinetic studies by ultra-pressure li


:A sensitive and reliable ultra-pressure liquid chromatography with tandem mass spectrometry (UPLC-MS) was developed and validated for simultaneous quantification of six main bioactive components, i.e., calycosin-7-O-β-D-glucoside, ononin, calycosin, formononetin, astragaloside IV, and astragaloside II in rat plasma after oral administration of the 95 % ethanol extraction from Radix Astragali. Plasma samples were extracted with Waters Oasis(TM) HLB 1 cc (30 mg) Extraction Cartridges (SPE) separated on an UPLC™ BEH C18 column and detected by MS with electro spray ionization interface in positive selective ion monitoring mode. Calibration curves offered linear ranges of two orders of magnitude with r (2) > 0.99. The method had the lower limit quantification of 1.30, 0.73, 1.17, 2.33, 0.63, and 0.83 ng/mL for ononin, calycosin, calycosin-7-O-β-D-glucoside, formononetin, astragaloside IV, and astragaloside II, respectively, with precision less than 10 %. The RSD of intra- and inter-day variations ranged from 1.66 to 6.46 and 3.39 to 6.58 %. This developed method was applied subsequently to pharmacokinetic studies of the six compounds in rats successfully. The proposed method was for the first time to compare the pharmacokinetic difference between calycosin-7-O-β-D-glucoside and calycosin in rat plasma, so as between ononin and formononetin, and studied to the astragaloside II pharmacokinetics in rat plasma.


Cell Biochem Biophys


Liu XH,Zhao JB,Guo L,Yang YL,Hu F,Zhu RJ,Feng SL




Has Abstract


2014-09-01 00:00:00












  • The Use of Calcium Channel Blockers in the Treatment of Coronary Spasm and Atrioventricular Block.

    abstract::Calcium channel blockers have been used in the treatment of coronary artery spasm for many years. However, there is insufficient knowledge about their application to treat atrioventricular block caused by coronary spasm. Clinical data of five patients who were diagnosed with coronary spasm caused by atrioventricular b...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gao B,Zhang Z,Qian J,Cao C,Hua X,Chu M,He X,Zeng H

    更新日期:2015-06-01 00:00:00

  • Comparison of Gene Expression Profile Between Tumor Tissue and Adjacent Non-tumor Tissue in Patients with Gastric Gastrointestinal Stromal Tumor (GIST).

    abstract::Gastrointestinal stromal tumors (GISTs) are defined as spindle cell and/or epithelioid tumors originated from interstitial Cajal cells or precursors in the digestive tract. This study was conducted to identify genes differing in expression between the gastric tumors and the adjacent non-cancerous mucosas in patients w...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kou Y,Zhao Y,Bao C,Wang Q

    更新日期:2015-06-01 00:00:00

  • Chemopreventive Effects of Azadirachta indica on Cancer Marker Indices and Ultrastructural Changes During 1,2-Dimethylhydrazine-Induced Colon Carcinogenesis in Rats.

    abstract::The present study elucidated the prospective of Azadirachta indica supplementation, if any, in affording chemoprevention by modulating the altered cancer markers and ultrastructural changes in DMH-induced colorectal carcinogenesis in rats. The rats were segregated into four groups viz., normal control, DMH treated, A....

    journal_title:Cell biochemistry and biophysics

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    authors: Liu N,Sun B,Wu P,Wei X

    更新日期:2015-09-01 00:00:00

  • Nursing research on a first aid model of double personnel for major burn patients.

    abstract::This study explored the effect of a first aid model employing two nurses on the efficient rescue operation time and the efficient resuscitation time for major burn patients. A two-nurse model of first aid was designed for major burn patients. The model includes a division of labor between the first aid nurses and the ...

    journal_title:Cell biochemistry and biophysics

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    authors: Wu W,Shi K,Jin Z,Liu S,Cai D,Zhao J,Chi C,Yu J

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  • IP-10/CXCR3 Axis Promotes the Proliferation of Vascular Smooth Muscle Cells through ERK1/2/CREB Signaling Pathway.

    abstract::Excessive proliferation of vascular smooth muscle cells is one of the main pathological processes leading to atherosclerosis and intimal hyperplasia after vascular interventional therapy. Our previous study has shown that interferon-γ inducible protein-10 contributes to the proliferation of vascular smooth muscle cell...

    journal_title:Cell biochemistry and biophysics

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    authors: Wang HJ,Zhou Y,Liu RM,Qin YS,Cen YH,Hu LY,Wang SM,Hu ZJ

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  • Influence of amino acids Shiff bases on irradiated DNA stability in vivo.

    abstract::To reveal protective role of the new Mn(II) complexes with Nicotinyl-L-Tyrosinate and Nicotinyl-L-Tryptophanate Schiff Bases against ionizing radiation. The DNA of the rats liver was isolated on 7, 14, and 30 days after X-ray irradiation. The differences between the DNA of irradiated rats and rats pre-treated with Mn(...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Karapetyan NH,Malakyan MH,Bajinyan SA,Torosyan AL,Grigoryan IE,Haroutiunian SG

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  • Studying single red blood cells under a tunable external force by combining passive microrheology with Raman spectroscopy.

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Raj S,Wojdyla M,Petrov D

    更新日期:2013-04-01 00:00:00

  • Extremely high frequency electromagnetic radiation enforces bacterial effects of inhibitors and antibiotics.

    abstract::The coherent electromagnetic radiation (EMR) of the frequency of 51.8 and 53 GHz with low intensity (the power flux density of 0.06 mW/cm(2)) affected the growth of Escherichia coli K12(lambda) under fermentation conditions: the lowering of the growth specific rate was considerably (approximately 2-fold) increased wit...

    journal_title:Cell biochemistry and biophysics

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    authors: Tadevosyan H,Kalantaryan V,Trchounian A

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  • The effects and mechanism of miR-92a and miR-126 on myocardial apoptosis in mouse ischemia-reperfusion model.

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    journal_title:Cell biochemistry and biophysics

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    authors: Jiang C,Ji N,Luo G,Ni S,Zong J,Chen Z,Bao D,Gong X,Fu T

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  • Analysis of Myocardial (18)F-FDG Uptake by PET/CT in the Patients with Different Dialectically Classified Coronary Heart Diseases (CHD).

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    authors: Tan YY,Liu DF,Zhu F,Ding XM,Wang GM

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    journal_title:Cell biochemistry and biophysics

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    authors: Yang P,Yang N,Zhang X,Xu X

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    authors: Anner BM,Volet B

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  • Effects of tumor necrosis factor inhibitor on serum level of HLA-B27 and PDCD-1 in patients with ankylosing spondylitis.

    abstract::The aim of this study was to evaluate the effect of tumor necrosis factor-alpha (TNF-α) inhibitor-infliximab on ankylosing spondylitis (AS) patients and detect the serum level of HLA-B27 and PDCD-1 before and after TNF inhibitor treatment. 138 patients at active stage of AS were treated with infliximab; serum was coll...

    journal_title:Cell biochemistry and biophysics

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    authors: Chen X,Zhou X,Li X,Tang J,Hu X,Wang J,Xu C

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  • An observational study of autologous bone marrow-derived stem cells transplantation in seven patients with nervous system diseases: a 2-year follow-up.

    abstract::Currently, autologous bone marrow-derived stem cell is one of the most innovative areas of stem cells research. Previous studies on animal models of nervous system diseases have shown that these cells have a good effect on nervous system disorders. The alternative treatment with stem cells for the nervous system disea...

    journal_title:Cell biochemistry and biophysics

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    authors: Ren C,Geng RL,Ge W,Liu XY,Chen H,Wan MR,Geng DQ

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  • Posterior fossa decompression combined with resection of the cerebellomedullary fissure membrane and expansile duraplasty: a radical and rational surgical treatment for Arnold-Chiari type I malformation.

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    journal_title:Cell biochemistry and biophysics

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    authors: Liang CJ,Dong QJ,Xing YH,Shan M,Wen LX,Qiang ZY,Ping ZQ,Tao PZ,Ping HX

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  • Acteoside Presents Protective Effects on Cerebral Ischemia/reperfusion Injury Through Targeting CCL2, CXCL10, and ICAM1.

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    journal_title:Cell biochemistry and biophysics

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    authors: Wu W,Wu G,Cao D

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    abstract::Nutritional changes can affect either the assembly or disassembly of yeast peroxisomes. In the past decade, insights regarding the molecular mechanisms of peroxisome assembly have been gained chiefly through the cloning of the PEX genes obtained by complementation of corresponding pex mutants in several yeast strains ...

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  • Human Airway Primary Epithelial Cells Show Distinct Architectures on Membrane Supports Under Different Culture Conditions.

    abstract::To facilitate drug development for lung delivery, it is highly demanding to establish appropriate airway epithelial cell models as transport barriers to evaluate pharmacokinetic profiles of drug molecules. Besides the cancer-derived cell lines, as the primary cell model, normal human bronchial epithelial (NHBE) cells ...

    journal_title:Cell biochemistry and biophysics

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    authors: Min KA,Rosania GR,Shin MC

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  • Calcium channel gamma subunits: a functionally diverse protein family.

    abstract::The calcium channel gamma subunits comprise an eight-member protein family that share a common topology consisting of four transmembrane domains and intracellular N- and C-termini. Although the first gamma subunit was identified as an auxiliary subunit of a voltage-dependent calcium channel, a review of phylogenetic, ...

    journal_title:Cell biochemistry and biophysics

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    authors: Chen RS,Deng TC,Garcia T,Sellers ZM,Best PM

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  • Interaction between umami peptide and taste receptor T1R1/T1R3.

    abstract::The umami taste receptor is a heterodimer composed of two members of the T1R taste receptor family: T1R1 and T1R3. The homology models of the ligand binding domains of the human umami receptor have been constructed based on crystallographic structures of the taste receptor of the central nervous system. Furthermore, t...

    journal_title:Cell biochemistry and biophysics

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    authors: Dang Y,Gao X,Xie A,Wu X,Ma F

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  • Expression and characterization of deletion recombinants of two cGMP-inhibited cyclic nucleotide phosphodiesterases (PDE-3).

    abstract::cDNAs encoding two PDE-3 or cyclic GMP-inhibited (cGI) cyclic nucleotide phosphodiesterase (PDE) isoforms, RPDE-3B (RcGIP1) and HPDE-3A (HcGIP2), were cloned from rat (R) adipose tissue and human (H) heart cDNA libraries. Deletion and N- and C-terminal truncation mutants were expressed in Escherichia coli in order to ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: He R,Komas N,Ekholm D,Murata T,Taira M,Hockman S,Degerman E,Manganiello VC

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  • Suitable depth of epidural puncture in nulliparous pregnant woman.

    abstract::We study the suitable depth for epidural puncture in primiparas so as to decrease epidural complications and provide anesthesiologists with an appropriate insertion guide. A prospective study of 87 primipara patients receiving labor analgesia who had epidural punctures in the course of vaginal delivery were randomly d...

    journal_title:Cell biochemistry and biophysics

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    authors: Luo Y,Zhang D,Zheng J,Yang Q,An X,Chen Y,Yu D,Yang Z

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  • Highly Conserved Arg Residue of ERFNIN Motif of Pro-Domain is Important for pH-Induced Zymogen Activation Process in Cysteine Cathepsins K and L.

    abstract::Pro-domain of a cysteine cathepsin contains a highly conserved Ex2Rx2Fx2Nx3Ix3N (ERFNIN) motif. The zymogen structure of cathepsins revealed that the Arg(R) residue of the motif is a central residue of a salt-bridge/H-bond network, stabilizing the scaffold of the pro-domain. Importance of the arginine is also demonstr...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Aich P,Biswas S

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  • Transforming growth factor-beta1 and disorders of the lung.

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    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Camoretti-Mercado B,Solway J

    更新日期:2005-01-01 00:00:00

  • Principles of macromolecular organization and cell function in bacteria and archaea.

    abstract::Structural organization of the cytoplasm by compartmentation is a well established fact for the eukaryotic cell. In prokaryotes, compartmentation is less obvious. Most prokaryotes do not need intracytoplasmic membranes to maintain their vital functions. This review, especially dealing with prokaryotes, will point out ...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Hoppert M,Mayer F

    更新日期:1999-01-01 00:00:00

  • Effect of miR-21 on renal fibrosis by regulating MMP-9 and TIMP1 in kk-ay diabetic nephropathy mice.

    abstract::MicroRNAs (miRs) play important roles in initiation and progression of many pathologic processes. However, the roles of miRs in diabetic nephropathy remain unclear. This study was to determine whether miR-21 was involved in diabetic nephropathy and to explore the relationship between miR-21 and MMP9/TIMP1 expression i...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wang J,Gao Y,Ma M,Li M,Zou D,Yang J,Zhu Z,Zhao X

    更新日期:2013-11-01 00:00:00

  • Association with leptin gene C.-2548 G>A polymorphism, serum leptin levels, and body mass index in Turkish obese patients.

    abstract::Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene (LEP), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefo...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sahin DS,Tumer C,Demir C,Celik MM,Celik M,Ucar E,Gunesacar R

    更新日期:2013-03-01 00:00:00

  • Functions and roles of a protein-associated factor.

    abstract::The divergence of protein (Hedgehog) in different organisms remains an unresolved issue to comprehend how the pathway in Hh signaling evolves. Insights into this question can help one identify the key molecules in Hh signaling. This work proposes a protein-associated factor in cells. The development of a non-reduction...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sarker MM,Zhou MC,Rameshwar P,Hanover JA

    更新日期:2014-04-01 00:00:00

  • Construction of small-caliber, polydioxanone cyclohexanone vascular stents.

    abstract::Objective of this article is to construct and characterize a three-layered small-caliber, artificial vascular stent. The outer layer of the stent consisted of small intestine submucosa (SIS), the middle layer was the polydioxanone (PDS) vascular stents, and the inner layer. The SIS and PDS were attached with 8-0 PDS t...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: You Q,Wang F,Duan L,Du X,Xiao M,Shen Z

    更新日期:2010-05-01 00:00:00

  • Analysis of volatile organic compounds liberated and metabolised by human umbilical vein endothelial cells (HUVEC) in vitro.

    abstract::Gas chromatography with mass spectrometric detection combined with head-space needle trap extraction as the pre-concentration technique was applied to identify and quantify volatile organic compounds released or metabolised by human umbilical vein endothelial cells. Amongst the consumed species there were eight aldehy...

    journal_title:Cell biochemistry and biophysics

    pub_type: 杂志文章


    authors: Mochalski P,Theurl M,Sponring A,Unterkofler K,Kirchmair R,Amann A

    更新日期:2015-01-01 00:00:00