Gramicidin perforated patch-clamp technique reveals glycine-gated outward chloride current in dissociated nucleus solitarii neurons of the rat.

Abstract:

:1. The inhibitory response of exogenously applied glycine was investigated in freshly dissociated rat nucleus tractus solitarii neurons under whole cell configuration using new perforated patch-clamp technique termed "gramicidin perforated patch technique," which maintains intact intracellular Cl- concentrations. 2. Using the gramicidin perforated patch technique, at a holding potential (VH) of -45 mV, glycine induced outward currents in a concentration-dependent manner with a EC50 of 4.0 x 10(-5) M and at a Hill coefficient of 1.5. In contrast, using the nystatin perforated patch technique, glycine induced inward currents at the same VH in a concentration-dependent manner with an EC50 of 4.9 x 10(-5) M and at a Hill coefficient of 1.2. 3. The glycine-induced outward currents were blocked by strychnine in a concentration dependent manner with an IC50 of 2.2 x 10(-8) M. The blockade was competitive. 4. The current-voltage relationship for the 10(-5) M glycine response showed a clear outward rectification. 5. Ten-fold change of extracellular Cl- with a large impermeable anion resulted in a 65 mV shift of the reversal potential of glycine-induced currents (EGly), indicating that the membrane behaves like a Cl- electrode in the presence of glycine. 6. The intracellular Cl- activity calculated from the EGly ranged from 7.3 to 18.2 mM, with a mean value of 13.3 mM. 7. The values of EGly in the individual neurons were significantly negative to the resting membrane potentials, suggesting the existence of active transport of Cl-.

journal_name

J Neurophysiol

authors

Rhee JS,Ebihara S,Akaike N

doi

10.1152/jn.1994.72.3.1103

subject

Has Abstract

pub_date

1994-09-01 00:00:00

pages

1103-8

issue

3

eissn

0022-3077

issn

1522-1598

journal_volume

72

pub_type

杂志文章
  • Resonance effect for neural spike time reliability.

    abstract::The spike timing reliability of Aplysia motoneurons stimulated by repeated presentation of periodic or aperiodic input currents is investigated. Two properties of the input are varied, the frequency content and the relative amplitude of the fluctuations to the mean (expressed as the coefficient of variation: CV). It i...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1998.80.3.1427

    authors: Hunter JD,Milton JG,Thomas PJ,Cowan JD

    更新日期:1998-09-01 00:00:00

  • Modulation of the human vestibuloocular reflex during saccades: probing by high-frequency oscillation and torque pulses of the head.

    abstract::1. We probed the gain and phase of the vestibuloocular reflex (VOR) during the execution of voluntary gaze saccades, with continuous oscillation or acceleration pulses, applied through a torque helmet. 2. Small-amplitude (< 1 degree), high-frequency (10-14 Hz) head oscillations in the horizontal or vertical plane were...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1996.76.5.3249

    authors: Tabak S,Smeets JB,Collewijn H

    更新日期:1996-11-01 00:00:00

  • Processing of interaural temporal disparities in the medial division of the ventral nucleus of the lateral lemniscus.

    abstract::The medial division of the ventral nucleus of the lateral lemniscus (VNLLm) contains a specialized population of neurons that is sensitive to interaural temporal disparities (ITDs), a potent cue for sound localization along the azimuth. Unlike many ITD-sensitive neurons elsewhere in the auditory system, neurons in the...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2002.88.2.666

    authors: Batra R,Fitzpatrick DC

    更新日期:2002-08-01 00:00:00

  • Corticotrophin-releasing factor augments the I(H) in rat hypothalamic paraventricular nucleus parvocellular neurons in vitro.

    abstract::The goal of this study was to characterize the effects of corticotrophin-releasing factor (CRF) on rat paraventricular nucleus (PVN) putative parvocellular neurons using whole cell patch-clamp recordings and single-cell reverse transcription-multiplex polymerase chain reaction (single-cell RT-mPCR) techniques. Under c...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01325.2004

    authors: Qiu DL,Chu CP,Shirasaka T,Tsukino H,Nakao H,Kato K,Kunitake T,Katoh T,Kannan H

    更新日期:2005-07-01 00:00:00

  • Altered desensitization produces enhancement of EPSPs in neocortical neurons.

    abstract::1. Neocortical brain slices were prepared from rats (35-50 days of age) and maintained in vitro. Intracellular recordings were obtained from neurons in cortical layers II/III. The effect of bath application of cyclothiazide (CYZ), a potent blocker of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1994.72.2.1032

    authors: Pelletier MR,Hablitz JJ

    更新日期:1994-08-01 00:00:00

  • Primate insular/opercular taste cortex: neuronal representations of the viscosity, fat texture, grittiness, temperature, and taste of foods.

    abstract::It is shown that the primate primary taste cortex represents not only taste but also information about many nontaste properties of oral stimuli. Of 1,122 macaque anterior insular/frontal opercular neurons recorded, 62 (5.5%) responded to oral stimuli. Of the orally responsive neurons, some (53%) represented the viscos...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00321.2004

    authors: Verhagen JV,Kadohisa M,Rolls ET

    更新日期:2004-09-01 00:00:00

  • Glycine receptors involved in synaptic transmission are selectively regulated by the cytoskeleton in mouse spinal neurons.

    abstract::Using whole cell patch-clamp recordings, we examined the effect of colchicine, a microtubule disrupter, on the properties of glycine receptors (GlyRs) in cultured spinal cord neurons. Confocal microscopy revealed that colchicine treatment effectively altered microtubule bundles and neuronal morphology. Application of ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00455.2001

    authors: van Zundert B,Alvarez FJ,Yevenes GE,Cárcamo JG,Vera JC,Aguayo LG

    更新日期:2002-01-01 00:00:00

  • Muscarinic control of dendritic excitability and Ca(2+) signaling in CA1 pyramidal neurons in rat hippocampal slice.

    abstract::The cholinergic system is critically involved in synaptic models of learning and memory by enhancing dendritic [Ca(2+)](i) signals. Diffuse cholinergic innervation suggests subcellular modulation of membrane currents and Ca(2+) signals. Here we use ion-selective microelectrodes to study spread of carbachol (CCh) after...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1999.82.4.1909

    authors: Egorov AV,Gloveli T,Müller W

    更新日期:1999-10-01 00:00:00

  • Spatiotemporal factors influence sound-source segregation in localization behavior.

    abstract::To program a goal-directed response in the presence of acoustic reflections, the audio-motor system should suppress the detection of time-delayed sources. We examined the effects of spatial separation and inter-stimulus delay on the ability of human listeners to localize a pair of broadband sounds in the horizontal pl...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00184.2020

    authors: van Bentum GC,van Wanrooij MM,Van Opstal AJ

    更新日期:2020-12-30 00:00:00

  • Calcium spike-mediated digital signaling increases glutamate output at the visual threshold of retinal bipolar cells.

    abstract::Most retinal bipolar cells (BCs) transmit visual input from photoreceptors to ganglion cells using graded potentials, but some also generate calcium or sodium spikes. Sodium spikes are thought to increase temporal precision of light-evoked BC signaling; however, the role of calcium spikes in BCs is not fully understoo...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00378.2014

    authors: Lipin MY,Vigh J

    更新日期:2015-01-15 00:00:00

  • Dopamine modulates the slow Ca(2+)-activated K+ current IAHP via cyclic AMP-dependent protein kinase in hippocampal neurons.

    abstract::1. The effects of dopamine on the slow Ca(2+)-dependent K+ current (IAHP; AHP, afterhyperpolarization) and spike frequency adaptation were studied by whole cell voltage-clamp and sharp microelectrode current-clamp recordings in rat CA1 pyramidal neurons in rat hippocampal slices. 2. Dopamine suppressed IAHP in a dose-...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1995.74.6.2749

    authors: Pedarzani P,Storm JF

    更新日期:1995-12-01 00:00:00

  • 5-HT modulation of hyperpolarization-activated inward current and calcium-dependent outward current in a crustacean motor neuron.

    abstract::1. Serotonergic modulation of a hyperpolarization-activated inward current, Ih, and a calcium-dependent outward current, Io(Ca), was examined in the dorsal gastric (DG) motor neuron, with the use of intracellular recording techniques in an isolated preparation of the crab stomatogastric ganglion (STG). 2. Hyperpolariz...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1992.68.2.496

    authors: Kiehn O,Harris-Warrick RM

    更新日期:1992-08-01 00:00:00

  • Slow oscillating population activity in developing cortical networks: models and experimental results.

    abstract::During early development neuronal networks express slow oscillating synchronized activity. The activity can be driven by several, not necessarily mutually exclusive, mechanisms. Each mechanism might have distinctive consequences for the phenomenology, formation, or sustainment of the early activity pattern. Here we st...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00889.2010

    authors: Baltz T,Herzog A,Voigt T

    更新日期:2011-09-01 00:00:00

  • The spinal control of locomotion and step-to-step variability in left-right symmetry from slow to moderate speeds.

    abstract::When speed changes during locomotion, both temporal and spatial parameters of the pattern must adjust. Moreover, at slow speeds the step-to-step pattern becomes increasingly variable. The objectives of the present study were to assess if the spinal locomotor network adjusts both temporal and spatial parameters from sl...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00419.2015

    authors: Dambreville C,Labarre A,Thibaudier Y,Hurteau MF,Frigon A

    更新日期:2015-08-01 00:00:00

  • Plasticity of response properties of inferior colliculus neurons following acute cochlear damage.

    abstract::1. The discharge patterns of 40 neurons in the central nucleus of the inferior colliculus (ICC) of the chinchilla were evaluated before and after acute cochlea trauma from intense tone exposure. Single-unit recordings were obtained from neurons in the ICC contralateral to the sound-stimulated ear. Cochlear trauma was ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1996.75.1.171

    authors: Wang J,Salvi RJ,Powers N

    更新日期:1996-01-01 00:00:00

  • Long-latency and voluntary responses to an arm displacement can be rapidly attenuated by perturbation offset.

    abstract::Feedback control of our limbs must account for the unexpected offset of mechanical perturbations. Here we examine the evoked activity of elbow flexor and extensor muscles to torque pulses lasting 22-152 ms and how torque offset impacts activity in the long-latency (45-100 ms) and voluntary epochs (120-180 ms). For eac...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01139.2009

    authors: Kurtzer I,Pruszynski JA,Scott SH

    更新日期:2010-06-01 00:00:00

  • Electrical interaction between motoneurons and afferent terminals in cat spinal cord.

    abstract::1. The electrical threshold of the terminations of single group Ia and II afferent fibers was transiently reduced by the antidromic discharge of the initial segment of spinal motoneurons in the cat. 2. This depolarization of the terminals of afferent fibers known to synapse on motoneurons confirms previous observation...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1979.42.3.635

    authors: Curtis DR,Lodge D,Headley PM

    更新日期:1979-05-01 00:00:00

  • Covert representation of second-next movement in the pre-supplementary motor area of monkeys.

    abstract::We attempted to analyze the nature of premovement activity of neurons in medial motor areas [supplementary motor area (SMA) and pre-SMA] from a perspective of coding multiple movements. Monkeys were trained to perform a series of two movements with an intervening delay: supination or pronation with either forearm. Mov...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.90636.2008

    authors: Nakajima T,Hosaka R,Mushiake H,Tanji J

    更新日期:2009-04-01 00:00:00

  • Neural mechanisms for sigh generation during prenatal development.

    abstract::The respiratory network of the preBötzinger complex (preBötC), which controls inspiratory behavior, can in normal conditions simultaneously produce two types of inspiration-related rhythmic activities: the eupneic rhythm composed of monophasic, low-amplitude, and relatively high-frequency bursts, interspersed with sig...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章,评审

    doi:10.1152/jn.00314.2018

    authors: Thoby-Brisson M

    更新日期:2018-09-01 00:00:00

  • Discharge patterns of neurons in the ventral nucleus of the lateral lemniscus of the unanesthetized rabbit.

    abstract::The ventral nucleus of the lateral lemniscus (VNLL) is a major auditory nucleus that sends a large projection to the inferior colliculus. Despite its prominence, the responses of neurons in the VNLL have not been extensively studied. Previous studies in nonecholocating species have used anesthesia, which is known to a...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1999.82.3.1097

    authors: Batra R,Fitzpatrick DC

    更新日期:1999-09-01 00:00:00

  • Early postnatal plasticity in neocortex of Fmr1 knockout mice.

    abstract::Fragile X syndrome is produced by a defect in a single X-linked gene, called Fmr1, and is characterized by abnormal dendritic spine morphologies with spines that are longer and thinner in neocortex than those from age-matched controls. Studies using Fmr1 knockout mice indicate that spine abnormalities are especially p...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00221.2006

    authors: Desai NS,Casimiro TM,Gruber SM,Vanderklish PW

    更新日期:2006-10-01 00:00:00

  • Decomposition of surface EMG signals from cyclic dynamic contractions.

    abstract::Over the past 3 decades, various algorithms used to decompose the electromyographic (EMG) signal into its constituent motor unit action potentials (MUAPs) have been reported. All are limited to decomposing EMG signals from isometric contraction. In this report, we describe a successful approach to decomposing the surf...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00555.2014

    authors: De Luca CJ,Chang SS,Roy SH,Kline JC,Nawab SH

    更新日期:2015-03-15 00:00:00

  • Impulse rates and sensitivity to stretch of soleus muscle spindle afferent fibers during locomotion in premammillary cats.

    abstract::Impulse from soleus muscle afferents were recorded in premammillary cats that were walking on a treadmill. In normal walking the effects of gamma-motoneurons on impulse rates of muscle spindle afferents are confounded by the effects of the large length changes that occur. To isolate the effects of gamma-motoneurons th...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1985.53.2.341

    authors: Taylor J,Stein RB,Murphy PR

    更新日期:1985-02-01 00:00:00

  • Accommodative reactions of medullary respiratory neurons of the cat.

    abstract::In most of the bulbospinal respiratory neurons, threshold depolarization increased during the early period of their spontaneous burst discharge but decreased again at the end of a burst. In some vagal respiratory neurons, however, threshold depolarization increased steadily until the very end of their discharge period...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1975.38.5.1172

    authors: Richter DW,Heyde F

    更新日期:1975-09-01 00:00:00

  • Influence of instruction, prediction, and afferent sensory information on the postural organization of step initiation.

    abstract::1. Our previous study showed that two distinct postural modifications occurred when subjects were instructed to step, rather than maintain stance, in response to a backward surface translation: 1) the automatic postural responses to the surfaces perturbation were reduced in magnitude and 2) the anticipatory postural a...

    journal_title:Journal of neurophysiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1152/jn.1996.75.4.1619

    authors: Burleigh A,Horak F

    更新日期:1996-04-01 00:00:00

  • Directional sensitivity of "first trial" reactions in human balance control.

    abstract::Support-surface movements are commonly used to examine balance control. Subjects typically receive a series of identical or randomly interspersed multidirectional balance perturbations and the atypical "first trial reaction" (evoked by the first perturbation) is often excluded from further analysis. However, this proc...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.90945.2008

    authors: Oude Nijhuis LB,Allum JH,Borm GF,Honegger F,Overeem S,Bloem BR

    更新日期:2009-06-01 00:00:00

  • Characteristics of a slow hyperpolarizing synaptic potential in rat hippocampal pyramidal cells in vitro.

    abstract::An orthodromically evoked late hyperpolarizing potential (LHP) was studied using intracellular recording techniques in rat hippocampal CA1 pyramidal cells in vitro. Several tests indicated that the LHP is not blocked by GABA antagonists, but rather comprises the initial portion of the stimulation-induced burst afterhy...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1984.52.5.892

    authors: Alger BE

    更新日期:1984-11-01 00:00:00

  • Sequestration of glutamate-induced Ca2+ loads by mitochondria in cultured rat hippocampal neurons.

    abstract::1. Buffering of glutamate-induced Ca2+ loads in single rat hippocampal neurons grown in primary culture was studied with ratiometric fluorescent Ca2+ indicators. The hypothesis that mitochondria buffer the large Ca2+ loads elicited by glutamate was tested. 2. The relationship between glutamate concentration and the re...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1996.76.3.1611

    authors: Wang GJ,Thayer SA

    更新日期:1996-09-01 00:00:00

  • Binaral interactions in the rat piriform cortex.

    abstract::Single-unit recordings were made from layer II/III anterior piriform cortex (aPCX) neurons in adult Wistar rats to examine odor response patterns to unilaterally and bilaterally delivered stimuli. Isoamyl acetate odor stimulation was presented either unilaterally through tubes inserted into the external nares, or bila...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1997.78.1.160

    authors: Wilson DA

    更新日期:1997-07-01 00:00:00

  • Proton-gated sodium current in parasympathetic ganglion cells of frog heart.

    abstract::1. The proton-gated current was investigated in whole-cell configuration of the neurons isolated from bullfrog heart parasympathetic ganglia with the use of the "concentration-clamp" technique, which combines intracellular perfusion and extremely rapid exchange of external solution within 1-2 ms, under a single-electr...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1990.63.5.1060

    authors: Kim DK,Tateishi N,Akaike N

    更新日期:1990-05-01 00:00:00