Abstract：
:There is growing evidence that lymphocyte trafficking contributes to the clinical course of chronic lymphocytic leukemia (CLL), but to date, only static in vitro cultures have been used to study these phenomena. To address this lack of data, we have developed a dynamic in vitro model in which CLL cells experience shear forces equivalent to those in capillary beds and are made to flow through capillary-like hollow fibers lined with endothelial cells. CLL cells treated in this way increased their expression of CD62L and CXCR4 (both P < .0001) and of CD49d and CD5 (both P = .003) directly as a result of the shear force. Furthermore, CLL cells migrated through the endothelium into the "extravascular" space (mean migration, 1.37% ± 2.14%; n = 21). Migrated CLL cells had significantly higher expression of CD49d (P = .02), matrix metallopeptidase-9 (P = .004), CD38 (P = .009), CD80 (P = .04), and CD69 (P = .04) compared with CLL cells that remained in the circulation. The degree of migration observed strongly correlated with CD49d expression (r(2), 0.47; P = .01), and treatment with the CD49d-blocking antibody natalizumab resulted in significantly decreased migration (P = .01). Taken together, our data provide evidence for a novel, dynamic, and tractable in vitro model of lymphocyte migration and confirm that CD49d is a critical regulator of this process in CLL.
journal_name
Bloodjournal_title
Bloodauthors
Walsby E,Buggins A,Devereux S,Jones C,Pratt G,Brennan P,Fegan C,Pepper Cdoi
10.1182/blood-2013-12-544569subject
Has Abstractpub_date
2014-06-05 00:00:00pages
3607-17issue
23eissn
0006-4971issn
1528-0020pii
blood-2013-12-544569journal_volume
123pub_type
杂志文章相关文献
BLOOD文献大全abstract：:Epithin/PRSS14, a type II transmembrane serine protease, is involved in normal epithelial development and tumor progression. Here we report, as an interacting substrate of epithin, a receptor tyrosine kinase Tie2 that is well known for important roles in the vessel stability. Epithin interacts with and degrades the Ti...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2010-03-275289
更新日期：2011-01-27 00:00:00
abstract：:Human promyelocytic leukemia (HL-60) cells were induced to differentiate into macrophage-like cells by treatment with 10(-7) mol/L 1,25-dihydroxyvitamin D3 (VD3). A monoclonal antibody (MoAb, 60B8), reactive with the particulate of the differentiated cells but not of the untreated cells, was isolated. The antigen reco...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:Both total metabolic tumor volume (TMTV), computed on baseline positron emission tomography (PET), and end of induction (EOI) PET are imaging biomarkers showing promise for early risk stratification in patients with high-tumor-burden follicular lymphoma. A model was built incorporating these 2 factors in 159 patients ...
journal_title：Blood
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1182/blood-2017-11-816298
更新日期：2018-05-31 00:00:00
abstract：:Multipotent adult progenitor cells (MAPCs) are bone marrow-derived stem cells that have extensive in vitro expansion capacity and can differentiate in vivo and in vitro into tissue cells of all 3 germinal layers: ectoderm, mesoderm, and endoderm. The origin of MAPCs within bone marrow is unknown. MAPCs are believed to...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2004-12-4603
更新日期：2005-11-15 00:00:00
abstract：:A novel Hodgkin's disease (HD) derived cell line, L1236, was established from the peripheral blood of a patient with advanced Hodgkin's disease. Analysis of immunoglobulin (Ig) gene rearrangements revealed a biallelic Ig heavy chain and a monoallelic Ig kappa light chain gene rearrangement, pointing to a B-lymphoid or...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-04-15 00:00:00
abstract：:Nomifensine (Merital, Alival; Hoechst, Frankfurt, FRG), an antidepressant drug, may cause immune hemolytic anemia (IHA) of the so-called immune complex type that is believed to occur by means of an innocent-bystander mechanism. In this report we describe findings that are not consistent with this mechanism in a patien...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:Exposure of neutrophils to agents such as lipopolysaccharide, tumor necrosis factor-alpha (TNF-alpha), and the granulocyte-macrophage colony-stimulating factor causes a major upregulation of subsequent agonist-induced NADPH oxidase activation. This priming effect is a prerequisite for neutrophil-mediated tissue damage...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:B-lymphoid kinase (Blk) is exclusively expressed in B cells and thymocytes. Interestingly, transgenic expression of a constitutively active form of Blk in the T-cell lineage of mice results in the development of T-lymphoid lymphomas. Here, we demonstrate nuclear factor-kappa B (NF-kappaB)-mediated ectopic expression o...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2008-09-181024
更新日期：2009-06-04 00:00:00
abstract：:Fibrin is essential for hemostasis; however, abnormal fibrin formation is hypothesized to increase thrombotic risk. We previously showed that in situ thrombin generation on a cell's surface modulates the 3-dimensional structure and stability of the fibrin network. Currently, we compared the abilities of extravascular ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2009-06-228940
更新日期：2009-11-26 00:00:00
abstract：:Factor IXAlabama is a variant factor IX molecule responsible for a clinically moderate form of hemophilia B. Twenty-five kilobases (kb) of the variant gene, including seven exons coding for the structural protein, were cloned and characterized. The restriction map and the arrangement of coding regions are identical to...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Bone morphogenetic proteins (BMPs), members of the transforming growth factor (TGF)-beta superfamily, are a group of related proteins that are capable of inducing the formation of cartilage and bone but are now regarded as multifunctional cytokines. We show in this report a novel function of BMPs in hematopoietic cell...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-09-15 00:00:00
abstract：:Obesity and inflammatory bowel disease (IBD) are systemic inflammatory disorders that predispose to arterial and venous thrombosis through similar prothrombotic mechanisms. Obesity and IBD are chronic risk factors that lead to a persistently elevated risk of thrombosis, although the thrombotic risk with IBD appears to...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2016-05-716720
更新日期：2016-11-17 00:00:00
abstract：:The purpose of this study was to characterize the stimulus that activates the 5-lipoxygenase pathway in human peripheral monocytes (PM) during the process of contact activation. Incubation of PM, but not of polymorphonuclear leukocytes (PMN), in contact-activated, recalcified plasma induced a time-dependent release of...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:In severe human beta-thalassemia, the pathophysiology relates to accumulation of excess alpha-globin chains at the membrane. One hypothesis is that membrane-associated alpha-globin by virtue of it's iron or hemichromes produces oxidation of adjacent membrane proteins. The availability of a mouse model of severe beta-t...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1992-02-15 00:00:00
abstract：:Protein S is a cofactor for tissue factor pathway inhibitor (TFPI), accelerating the inhibition of activated factor X (FXa). TFPI Kunitz domain 3 residue Glu226 is essential for enhancement of TFPI by protein S. To investigate the complementary functional interaction site on protein S, we screened 44 protein S point, ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2014-01-551812
更新日期：2014-06-19 00:00:00
abstract：:Approximately 20% of children with acute lymphoblastic leukemia (ALL) relapse because of failure to eradicate the disease. Current drug efficacy studies focus on reducing leukemia cell burden. However, if drugs have limited effects on leukemia-initiating cells (LICs), then these cells may expand and eventually cause r...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2012-08-448852
更新日期：2013-02-21 00:00:00
abstract：:Interleukin-1 beta (IL-1 beta) converting enzyme (ICE) is a cysteine protease that specifically cleaves precursor IL-1 beta to its biologically active form. Recent studies have also implicated ICE in the induction of apoptosis in vertebrate cells. Because IL-1 plays a major role in acute myelogenous leukemia (AML) bla...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1995-12-15 00:00:00
abstract：:Despite matching for serologically defined HLA-A, B, DR antigens, acute graft-versus-host disease (GVHD) is a major complication contributing to increased morbidity and mortality in patients who undergo marrow transplantation from unrelated donors. The extent to which unrecognized mismatching for alleles that encode D...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1995-08-15 00:00:00
abstract：:Toll-like receptors (TLRs) are able to interact with pathogen-derived products and their signals induce the coordinated activation of innate and adaptive immune mechanisms. Dendritic cells (DCs) play a central role in these events. As the different TLRs are able to trigger MyD88/TRIF-dependent and -independent signali...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2005-10-4015
更新日期：2006-07-15 00:00:00
abstract：:The expression of Pax5 commits common lymphoid progenitor cells to B-lymphoid lineage differentiation. Little is known of possible variations in the levels of Pax5 expression and their influences on hematopoietic development. We have developed a retroviral transduction system that allows for the study of possible inte...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2012-03-414821
更新日期：2012-11-01 00:00:00
abstract：:Pediatric arterial ischemic stroke (AIS) is increasingly diagnosed and carries significant risks of recurrence, morbidity, and mortality. Anticoagulant therapy (ACT) is commonly prescribed in childhood AIS. Hemorrhagic complication rates in pediatric stroke are unknown, and adult safety data are of limited applicabili...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-06-361535
更新日期：2012-01-26 00:00:00
abstract：:Studies in mice have shown that proinflammatory Th17 cells can cause acute graft-versus-host disease (aGVHD) related tissue damage; however, whether they play a role in human aGVHD remains unclear. In a prospective study, we measured the proportion of Th17 cells in the blood and skin of patients at the onset of aGVHD....
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2010-07-295436
更新日期：2010-12-16 00:00:00
abstract：:CD10/neutral endopeptidase 24.11 (NEP) regulates peptidemediated proliferation of lymphoid progenitors and certain epithelial cells and is itself regulated by cellular proliferation. To further characterize mechanisms by which cell-surface signaling might regulate CD10/NEP expression, we determined whether CD10/NEP wa...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:The efficacy and safety of zanolimumab in patients with refractory cutaneous T-cell lymphoma (CTCL) have been assessed in two phase 2, multicenter, prospective, open-label, uncontrolled clinical studies. Patients with treatment refractory CD4(+) CTCL (mycosis fungoides [MF], n = 38; Sézary syndrome [SS], n = 9) receiv...
journal_title：Blood
pub_type： 杂志文章,多中心研究
doi：10.1182/blood-2006-12-062877
更新日期：2007-06-01 00:00:00
abstract：:One hundred thirty-eight patients with hairy cell leukemia were randomized to receive either a dose of 2.0 megaunits (MU)/m2 or a 10-fold lower dose of 0.2 MU/m2 of a highly purified natural alpha-interferon, administered daily for 28 days followed by a three times a week schedule. Ninety-seven of these patients had p...
journal_title：Blood
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1991-12-15 00:00:00
abstract：:Beginning in 1985, patients in British Columbia with Hodgkin lymphoma (HL) that was not controlled by conventional chemotherapy routinely underwent high-dose chemotherapy and autologous stem cell transplantation (HD-ASCT). Long-term complications of HD-ASCT have become apparent as more patients survive without recurre...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2004-12-4689
更新日期：2005-08-15 00:00:00
abstract：:The inclusion of exon 16 in the mature protein 4.1R messenger RNA (mRNA) is a critical event in red blood cell membrane biogenesis. It occurs during late erythroid development and results in inclusion of the 10-kd domain needed for stabilization of the spectrin/actin lattice. In this study, an experimental model was e...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood.v98.13.3809
更新日期：2001-12-15 00:00:00
abstract：:Cancer patients are at increased risk of deep vein thrombosis and pulmonary embolism. The incidence among different groups of cancer patients varies considerably depending on clinical factors, the most important being tumor entity and stage. Biomarkers have been specifically investigated for their capacity of predicti...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2013-04-460147
更新日期：2013-09-19 00:00:00
abstract：:The alpha thalassemias are associated with a decrease in alpha chain synthesis. Hemoglobin H (HbH) disease is a moderately severe form of alpha thalassemia characterized by the production of 5%--20% of HbH, while alpha thalassemia trait is a milder form of alpha thalassemia. In two patients with HbH disease, the ratio...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1976-06-01 00:00:00
abstract：:Human T cells were studied with regard to the regulation of interleukin-4 (IL-4) and IL-3 gene expression. IL-4 and IL-3 mRNA were undetectable in unstimulated T cells. On activation with the lectin concanavalin A (Con A), both IL-4 and IL-3 mRNA were expressed. Accumulation of IL-4 mRNA peaked after 6 to 12 hours, wh...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
