Abstract:
:The activities of four purified human growth factors: biosynthetic (recombinant) granulocyte-macrophage colony-stimulating factor (GM-CSF); recombinant erythroid-potentiating activity (EPA); natural and recombinant pluripoietin (Ppo); and natural pluripoietin alpha (Ppo alpha), were compared on the growth of hematopoietic colonies from enriched populations of human marrow and blood progenitor cells. Conditioned medium from the Mo T cell line (MoCM) was used as a standard positive control. We found that activities of GM-CSF and Ppo alpha on the growth of hematopoietic colonies were indistinguishable; Ppo alpha is now believed to be identical to GM-CSF. Both factors were able to promote the growth of colonies derived from subpopulations of CFU-GM, BFU-E, and CFU-GEM. Colonies derived from CFU-GM and CFU-GEM in cultures stimulated by GM-CSF and Ppo alpha were much smaller than in cultures stimulated by MoCM. In contrast to previous reports in which less highly enriched progenitors were used as target cells, Ppo had no detectable activity on the growth of colonies derived from BFU-E or CFU-GEM but promoted the growth of a subpopulation of CFU-GM derived colonies. Ppo is now recognized to be identical to G-CSF. The GM colonies in cultures stimulated by G-CSF (Ppo) were much smaller than in cultures stimulated by MoCM. EPA had no detectable activity on either the size or number of colonies derived from CFU-GM, BFU-E, or CFU-GEM. Results from experiments using target cell populations of marrow fractions separated by velocity sedimentation and marrow populations following freezing suggested that GM-CSF (Ppo alpha) and G-CSF (Ppo) primarily affect the growth of relatively mature subpopulations of progenitor cells. It is clear from these results that additional factor(s) are present in MoCM that are necessary to stimulate CFU-GM, BFU-E, and CFU-GEM maximally in vitro.
journal_name
Bloodjournal_title
Bloodauthors
Strife A,Lambek C,Wisniewski D,Gulati S,Gasson JC,Golde DW,Welte K,Gabrilove JL,Clarkson Bsubject
Has Abstractpub_date
1987-05-01 00:00:00pages
1508-23issue
5eissn
0006-4971issn
1528-0020journal_volume
69pub_type
杂志文章相关文献
BLOOD文献大全abstract::Crystallographic structures indicate that gamma-chain residue Asn308 participates in D:D interactions and indeed substitutions of gammaAsn308 with lysine or isoleucine have been identified in dysfibrinogens with impaired polymerization. To probe the role of Asn308 in polymerization, we synthesized 3 variant fibrinogen...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-12-4296
更新日期:2004-06-01 00:00:00
abstract::Surface receptors specific for either the C4b (CR1) or C3d (CR2) component of complement were examined on the neoplastic cells from 30 cases of non-Hodgkin's lymphoma of B-cell origin and on cells derived from 9 normal lymphoid tissues. Lymphocyte suspensions from non-neoplastic peripheral blood, tonsils, and lymph no...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::We report a novel, reproducible methodology which enabled 10 human myeloma cell lines (HMCL) to be obtained from each of 10 tumor samples harvested from 9 patients with extramedullary proliferation. Fresh samples were cultured with interleukin 6 (IL-6) and granulocyte macrophage-colony stimulating factor (GM-CSF) at a...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-06-15 00:00:00
abstract::The hematopoietically expressed homeobox gene, Hhex, is a transcription factor that is important for development of definitive hematopoietic stem cells (HSCs) and B cells, and that causes T-cell leukemia when overexpressed. Here, we have used an Hhex inducible knockout mouse model to study the role of Hhex in adult he...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-06-579813
更新日期:2015-01-29 00:00:00
abstract::Fetal hemoglobin (HbF) and adult hemoglobin (HbA) synthesis was studied in fetal baboons, Papio cynocephalus, to determine the normal pattern of hemoglobin production during fetal development. Fetuses ranging from 53 to 180 days gestation (term gestation 184 days) were used. Erythroid cells were incubated with 3H-L-le...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::Congenital dyserythropoietic anemia type II (CDA-II) is an autosomal recessive disease characterized by anemia, jaundice, splenomegaly, and erythroblast multinuclearity. The natural history of the disease is unknown. The frequency, the relevance of complications, and the use of splenectomy are poorly defined. This stu...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.4.1258
更新日期:2001-08-15 00:00:00
abstract::Growing evidence supports a pathophysiological role for platelets during the manifestation of postischemic reperfusion injury; in the current study, we investigated the nature and the molecular determinants of platelet-endothelial cell interactions induced by ischemia/reperfusion (I/R). Platelet-endothelium and leukoc...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-07-15 00:00:00
abstract::Folylpoly-gamma-gluatamate synthetase (FPGS) catalyzes the polyglutamylation and thus intracellular retention of folates and antifolates (eg, methotrexate; MTX) through the addition of multiple glutamate equivalents to their gamma-carboxyl residue. Since polyglutamylation of antifolates is crucial for their pharmacolo...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-08-173799
更新日期:2009-04-30 00:00:00
abstract::CCAAT displacement protein (cux/CDP) is an atypical homeodomain protein that represses expression of several developmentally regulated lymphoid and myeloid genes in vitro, including gp91-phox, immunoglobulin heavy chain, the T-cell receptor beta and gamma chains, and CD8. To determine how this activity affects cell de...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.13.3658
更新日期:2001-12-15 00:00:00
abstract::Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. Th...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-04-348748
更新日期:2011-09-29 00:00:00
abstract::Cell cycle checkpoints ensure orderly progression of events during cell division. A microtubule damage (MTD)-induced checkpoint has been described in G(1) phase of the cell cycle (G(1)MTC) for which little is known. The present study shows that the G(1)MTC is intact in activated T lymphocytes from mice with the p21(wa...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.5.1505
更新日期:2001-03-01 00:00:00
abstract::Surprisingly little is known about the interaction of human blood mononuclear cells with viruses. Here, we show that monocytes are the predominant cell type infected when peripheral blood mononuclear cells are exposed to viruses ex vivo. Remarkably, infection with vesicular stomatitis virus, vaccinia virus, and a vari...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-09-379479
更新日期:2012-03-29 00:00:00
abstract::In patients with chronic myeloid leukemia, BCR-ABL mutations contribute to resistance to tyrosine kinase inhibitor therapy. We examined the occurrence of treatment-emergent mutations and their impact on response in patients from the ENESTnd phase 3 trial. At the 3-year data cutoff, mutations were detected in approxima...
journal_title:Blood
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1182/blood-2012-04-423418
更新日期:2013-05-02 00:00:00
abstract::We used our recently reported stable, transformed human renal carcinoma cell line as a model system to study the role of 3',5'-adenosine monophosphate (cAMP) in erythropoietin secretion. The erythropoietin produced by these cells is both biologically active and immunologically cross-reactive with purified native human...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-04-01 00:00:00
abstract::Pseudomonas aeruginosa pneumonia was produced in dogs with radiation-induced leukopenia to study the comparative efficacy of several different therapies. In a randomized control trial, five treatment regimens were compared: no antibiotics or granulocytes (controls), gentamicin (5 mg/kg/day), carbenicillin (500 mg/kg/d...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1976-05-01 00:00:00
abstract::The International Immune Tolerance Study was a multicenter, prospective, randomized comparison of high-dose (HD; 200 IU/kg/d) and low-dose (LD; 50 IU/kg 3 times/week) factor VIII regimens in 115 "good-risk," severe high-titer inhibitor hemophilia A subjects. Sixty-six of 115 subjects reached the defined study end poin...
journal_title:Blood
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1182/blood-2011-08-369132
更新日期:2012-02-09 00:00:00
abstract::Evidence supporting the efficacy of in utero hematopoietic cell transplantation (IUHCT) in a valid large animal model is needed prior to clinical application. The objective of this study was to establish clinically relevant levels of hematopoietic chimerism in a canine model of maternal-to-fetal IUHCT. We first assess...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-11-537571
更新日期:2014-09-18 00:00:00
abstract::The adoptive transfer of donor T cells that recognize recipient minor histocompatibility antigens (mHAgs) is a potential strategy for preventing or treating leukemic relapse after allogeneic hematopoietic cell transplantation (HCT). A total of 7 patients with recurrent leukemia after major histocompatibility complex (...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-10-248997
更新日期:2010-05-13 00:00:00
abstract::Human fibrinogen 1 is homodimeric with respect to its gamma chains (gammaA-gammaA'), whereas fibrinogen 2 molecules each contain one gammaA (gammaA1-411V) and one gamma' chain, which differ by containing a unique C-terminal sequence from gamma'408 to 427L that binds thrombin and factor XIII. We investigated the struct...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-01-0240
更新日期:2005-10-15 00:00:00
abstract::Chronic lymphocytic leukemia (CLL) lymphocytes manifest anomalous motility and cap formation. Since these processes involve cytoskeletal proteins, vimentin from intermediate filaments of normal and CLL lymphocytes was investigated using hetero- and monoclonal antisera. The antisera reacted predominantly with a 60-kD p...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-02-01 00:00:00
abstract::The beta-globin locus control region (LCR) is a large DNA element that is required for high-level expression of beta-like globin genes from the endogenous mouse locus or in transgenic mice carrying the human beta-globin locus. The LCR encompasses 6 DNaseI hypersensitive sites (HSs) that bind transcription factors. The...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-06-2308
更新日期:2006-01-15 00:00:00
abstract::Factor VIII procoagulant protein (VIII:C) purified from commercial factor VIII concentrate contained multiple polypeptides ranging in mol wt from 79,000 to 188,000, all of which were removed from solution by a monoclonal anti-VIII:C antibody specific for a thrombin-sensitive epitope. In a time-course digest of the pur...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1983-04-01 00:00:00
abstract::Overexpression of transcription factors runt-related transcription factor 1 (RUNX1) and nuclear factor, erythroid-derived 2 (NF-E2) was reported in granulocytes of patients with polycythemia vera and other myeloproliferative neoplasms (MPNs). Further, a transgenic mouse overexpressing the NF-E2 transgene was reported ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-10-534222
更新日期:2014-01-16 00:00:00
abstract::In this issue of Blood, Larocca et al in a large prospective study show that thromboprophylaxis with low-dose aspirin is very effective in previously untreated myeloma patients with a low thromboembolic risk receiving lenalidomide with low-dose dexamethasone. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2011-08-375089
更新日期:2012-01-26 00:00:00
abstract::Human herpesvirus 6 activity (HHV-6) was studied in 15 allogeneic and 11 autologous marrow transplantation patients. After transplantation, HHV-6 was isolated from the peripheral blood mononuclear cells of 12 of 26 patients (6 allogeneic and 6 autologous). All isolates were variant B. Eleven of 26 and 12 of 19 patient...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-06-15 00:00:00
abstract::Mitochondrial clearance is a well recognized but poorly understood biologic process, and reticulocytes, which undergo programmed mitochondrial clearance, provide a useful model to study this phenomenon. At the ultrastructural level, mitochondrial clearance resembles an autophagy-related process; however, the role of a...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-04-151639
更新日期:2009-07-02 00:00:00
abstract::Daratumumab, a monoclonal CD38 antibody, is approved in the treatment of myeloma, but its efficacy and safety in light-chain (AL) amyloidosis has not been formally studied. This prospective phase 2 trial of daratumumab monotherapy for the treatment of AL amyloidosis was designed to determine the safety, tolerability, ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019004436
更新日期:2020-04-30 00:00:00
abstract::In this study human mononuclear phagocytes from the bone marrow (promonocytes and monocytes), peripheral blood monocytes, and tissue macrophages from the skin and the peritoneal cavity were studied with respect to their morphological, cytochemical, and functional characteristics, cell surface receptors, and 3H-thymidi...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
abstract::Dickkopf-1 (DKK1), a soluble inhibitor of Wnt signaling secreted by multiple myeloma (MM) cells contributes to osteolytic bone disease by inhibiting the differentiation of osteoblasts. In this study, we tested the effect of anti-DKK1 therapy on bone metabolism and tumor growth in a SCID-rab system. SCID-rab mice were ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-09-047712
更新日期:2007-03-01 00:00:00
abstract::The possibility of primitive hematopoietic cell ex vivo expansion is of interest for both gene therapy and transplantation applications. The engraftment of autologous rhesus peripheral blood (PB) progenitors expanded 10 to 14 days were tracked in vivo using genetic marking. Stem cell factor (SCF)/granulocyte colony-st...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-08-15 00:00:00