Abstract:
:The present study examined the effect of elevated glucose on glomerular cell membrane lipid peroxidation. Glomeruli were isolated from male Sprague-Dawley rat kidneys utilizing a standard sieving method and incubated in medium containing different concentrations of glucose at 37 degrees C for one and 48 hours. The levels of lipid peroxides (LPO) in the glomeruli were quantitated by malondialdehyde-thiobarbituric acid adduct formation. The levels of LPO in the glomeruli were significantly increased after one hour of exposure to high glucose (HG; 30 mM) medium, and this increase was sustained after 48 hours of exposure to HG. In contrast, osmotic control media containing either L-glucose or mannitol did not increase glomerular LPO. Dimethylthiourea (20 mM), a hydroxyl radical scavenger, completely blocked the increase of LPO in the glomeruli exposed to HG not only for one hour but also for 48 hours without altering LPO levels in the glomeruli exposed to control glucose. This provides evidence that oxidative stress can be induced by high ambient concentrations of glucose. Pretreatment with PKC antagonists, either 500 microM H-7 or 100 nM staurosporine, prevented the increase of LPO in the glomeruli exposed to HG for one hour but not in glomeruli exposed for 48 hours. These data suggest that (i) elevated glucose levels per se can cause oxidative stress and augment membrane lipid peroxidation of glomeruli, and (ii) activation of PKC may play a role in early phase of glucose-induced glomerular lipid peroxidation.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Ha H,Yoon SJ,Kim KHdoi
10.1038/ki.1994.461subject
Has Abstractpub_date
1994-12-01 00:00:00pages
1620-6issue
6eissn
0085-2538issn
1523-1755pii
S0085-2538(15)58742-5journal_volume
46pub_type
杂志文章abstract::IgG4-related disease is a recently recognized multi-organ disorder characterized by high levels of serum IgG4 and dense infiltration of IgG4-positive cells into several organs. Although the pancreas was the first organ recognized to be affected by IgG4-related disorder in the syndrome of autoimmune pancreatitis, we pr...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.2010.271
更新日期:2010-11-01 00:00:00
abstract::Accumulating evidence indicates that asymptomatic hyperuricemia is involved in the development of hypertension and chronic kidney disease. A 2-hit model has been proposed to explain the role of urate in hypertension. The first hit entails activation of the renin-angiotensin system and inhibition of nitric oxide synthe...
journal_title:Kidney international
pub_type: 杂志文章,评审
doi:10.1016/j.kint.2020.05.056
更新日期:2020-11-01 00:00:00
abstract::Chronic kidney disease (CKD) is usually associated with interstitial leukocytic cell infiltrates, which may contribute to disease progression by production of proinflammatory, proapoptotic, and profibrotic mediators. Recruiting leukocytes into the kidney involves local expression of chemotactic cytokines, that is, che...
journal_title:Kidney international
pub_type: 杂志文章,评审
doi:10.1038/sj.ki.5000053
更新日期:2006-01-01 00:00:00
abstract::The members of the matrix metalloproteinase gene family play critical roles in numerous physiologic events, including cellular migration, tissue remodeling in wound healing and development, as well as in the evolution of the inflammatory process. The 72 kDa gelatinase A (formerly denoted 72 kDa Type IV collagenase) is...
journal_title:Kidney international
pub_type: 杂志文章,评审
doi:10.1038/ki.1997.191
更新日期:1997-05-01 00:00:00
abstract::Despite the increasing knowledge of the role of gangliosides in normal and diseased tissues, little is known of the presence, distribution and functions of these molecules in the kidney. In this study we analyzed the main gangliosides of isolated glomeruli and cortical, medullary and papillary fractions of the human, ...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1994.14
更新日期:1994-01-01 00:00:00
abstract::Many transgenic and knockout mice with increased urine flow have structural abnormalities of the renal pelvis and inner medulla. Here, we used high resolution contrast enhanced T1-weighted magnetic resonance imaging of mice whose urea transporters UT-A1 and UT-A3 were deleted (UT-A1/3(-/-) mice) as a model for the in ...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.2008.392
更新日期:2008-11-01 00:00:00
abstract::Monoclonal antibodies (mAb) reactive against complement components involved in the classical activation pathway were applied in an indirect immunoperoxidase technique for the histological study of normal and diseased human renal tissues. Prominent staining with antibodies against the C4d fragment was seen in all glome...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1989.302
更新日期:1989-12-01 00:00:00
abstract::In April 2006, Japan's health insurance system instituted a bundling policy that included recombinant human erythropoietin (rHuEPO) in outpatient hemodialysis therapy. To evaluate outcomes of this, we analyzed a prospective cohort of hemodialysis patients in the Japan Dialysis Outcomes and Practice Patterns Study, in ...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.2010.382
更新日期:2011-02-01 00:00:00
abstract::Since the kidney medulla was reported to generate platelet activating factor (PAF), we investigated a possible effect of this agent on the reabsorptive function of in vitro microperfused medullary thick ascending limbs from mouse kidney (mTAL). PAF, 10(-7) M in the bath, significantly decreased the net chloride flux (...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1992.38
更新日期:1992-02-01 00:00:00
abstract::The renal 11beta-hydroxysteroid dehydrogenase type 2 (11betaHSD2) enzyme inactivates 11-hydroxy steroids in the kidney, thus protecting the nonselective mineralocorticoid receptor (MR) from occupation by glucocorticoids. The gene is highly expressed in all sodium-transporting epithelia, but also in human placenta, pan...
journal_title:Kidney international
pub_type: 杂志文章,评审
doi:10.1046/j.1523-1755.2000.00978.x
更新日期:2000-04-01 00:00:00
abstract::There is an urgent need for therapeutic agents that target humoral alloimmunity in solid organ transplantation. This includes sensitized patients with preformed donor-specific human leukocyte antigen antibodies and patients who develop de novo donor-specific antibodies, both of which are associated with acute and chro...
journal_title:Kidney international
pub_type: 杂志文章,评审
doi:10.1016/j.kint.2018.10.028
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Strict glycemic control and antihypertensive treatment may decrease but not eliminate the risk of progressive nephropathy in diabetic patients. C-peptide has been shown to exert beneficial effects on complications, including incipient nephropathy, in type 1 diabetes. METHODS:Renal effects of 14 days of intr...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1046/j.1523-1755.2001.00964.x
更新日期:2001-10-01 00:00:00
abstract:BACKGROUND:Wegener's granulomatosis (WG) is a rare systemic autoimmune disease characterized by small-vessel vasculitis leading to organ damage and the presence of antineutrophil cytoplasmic autoantibodies (ANCAs). ANCAs were shown to be involved in the pathogenesis of the disease by increasing adhesion of polymorphonu...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1046/j.1523-1755.2000.00430.x
更新日期:2000-12-01 00:00:00
abstract::Human transitional B cells express relatively high IL-10 and low TNF-α levels, which correlate with B regulatory activity in vitro. Herein, we aim to further define B regulatory phenotype and determine whether B regulatory activity can serve as a prognostic marker for renal allograft dysfunction (graft loss or 2-fold ...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1016/j.kint.2016.08.028
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:To chart the epidemiology of primary glomerular disease by means of a prospective regional study in the southern part of The Netherlands. METHODS:Experienced renal technicians collected renal biopsies, blood, and 24-hour urine samples at the bed site in each of the participating hospitals. The material was ...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1111/j.1523-1755.2004.00835.x
更新日期:2004-09-01 00:00:00
abstract::Unilateral ureteral obstruction results in marked changes in renal function throughout the nephron, including impaired acid and potassium secretion and salt wastage. The nephron site believed responsible for the acidification defect is the collecting duct. It has been presumed, although not demonstrated, that the cell...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1990.11
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Tumor necrosis factor (TNF) receptor family members, such as Fas and TNF receptor 1 (TNFR1), are thought to induce apoptosis in a variety of cells and organs. Although a number of potential scenarios have been postulated for the involvement of these receptors in the pathogenesis of acute renal failure (ARF),...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1046/j.1523-1755.2003.00709.x
更新日期:2003-01-01 00:00:00
abstract::Osteonectin (SPARC, culture shock protein, BM-40) is a widely distributed glycoprotein which binds calcium and several extracellular matrix proteins, including interstitial collagens and thrombospondin, but whose physiologic role remains undefined. In the present studies, we have demonstrated that immunoreactive osteo...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1992.8
更新日期:1992-01-01 00:00:00
abstract::Propofol has been shown to provide protection against renal ischemia/reperfusion injury experimentally, but clinical evidence is lacking. Here we studied the effect of propofol anesthesia on the occurrence of acute kidney injury following heart surgery with cardiopulmonary bypass. One hundred and twelve patients who u...
journal_title:Kidney international
pub_type: 杂志文章,随机对照试验
doi:10.1038/ki.2013.532
更新日期:2014-08-01 00:00:00
abstract::The hypercalcemia of malignancy is mediated by complex and heterogeneous mechanisms. Once thought of as a simple paraneoplastic syndrome mediated by the effects of tumor production of PTH, it is now clear that multiple mechanisms are involved and that these mechanisms involve abnormalities in calcium transport in bone...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1987.21
更新日期:1987-01-01 00:00:00
abstract::Identifying subjects with progressive chronic kidney disease will be important both in clinical practice and in conducting clinical trials. Pruijm et al. (in this issue) demonstrate for the first time that cortical oxygenation as evaluated by blood oxygenation level-dependent magnetic resonance imaging can predict fut...
journal_title:Kidney international
pub_type: 评论,杂志文章
doi:10.1016/j.kint.2017.11.029
更新日期:2018-04-01 00:00:00
abstract::The remnant kidney (RK) exhibits an enhanced fractional excretion of phosphate (FEPi) even in the absence of parathyroid hormone (PTH). Thus, factors other than PTH contribute to this adaptive phosphaturia. Dopamine (DA) infusion is phosphaturic, whereas stimulation of adrenoreceptors is antiphosphaturic. Therefore, t...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1993.144
更新日期:1993-05-01 00:00:00
abstract::To investigate mechanisms underlying GFR control in diabetes mellitus, renal hemodynamics and segmental tubular handling of sodium, using lithium clearance, were assessed in 41 insulin-dependent diabetics (IDD) treated by insulin for 11 +/- 8 days, and in 19 normal controls. Average GFR and effective renal plasma flow...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1990.95
更新日期:1990-04-01 00:00:00
abstract::Bone marrow (BM) cells are reported to contribute to the process of regeneration following myocardial infarction. The present study examined two independent clonal studies to determine the origin of bone marrow (BM)-derived cardiomyocytes. First, we transplanted single CD34(-)c-kit(+)Sca-1(+)lineage(-) side population...
journal_title:Kidney international
pub_type: 杂志文章,评审
doi:10.1111/j.1523-1755.2005.00624.x
更新日期:2005-11-01 00:00:00
abstract::Many renal diseases are associated with fibrin deposition in the glomeruli, a situation that reflects an abnormality in the balance between the coagulation and fibrinolytic systems. We recently demonstrated that normal mouse kidney contains very low levels of type 1 plasminogen activator inhibitor (PAI-1), a potent an...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1995.17
更新日期:1995-01-01 00:00:00
abstract::Recently, 4 new risk scores for the prediction of mortality and cardiovascular events were especially tailored for hemodialysis patients; these scores performed much better than previous scores. Tripepi et al. found that these risk scores were even more predictive for all-cause and cardiovascular death than the measur...
journal_title:Kidney international
pub_type: 评论,杂志文章
doi:10.1016/j.kint.2016.11.022
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme activated by strand breaks in DNA, plays an important role in the development of ischemia/reperfusion (I/R) injury. The aim of this study was to investigate the effects of a water-soluble and potent PARP inhibitor, 5-aminoisoquinolinone (5-AIQ), on the re...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1111/j.1523-1755.2004.00415.x
更新日期:2004-02-01 00:00:00
abstract::We examined the effect of daily cyclosporin A administration on the development and extent of tubulointerstitial nephritis produced in rats immunized with tubular basement membranes in adjuvant. Six mg/kg/day of cyclosporin A, given from the time of immunization, completely blocked the development of interstitial lesi...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1988.119
更新日期:1988-06-01 00:00:00
abstract::The thick ascending limb of Henle's loop ( TAL ) develops a specific and consistent structural lesion during perfusion of the isolated rat kidney that progresses from mitochondrial swelling at 15 min to complete cellular disruption at 90 min. Because it seemed possible that damage localized to this metabolically activ...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1984.9
更新日期:1984-01-01 00:00:00
abstract::Levels of prepro epidermal growth factor (EGF) mRNA in renal cortical tissue and urinary EGF excretion have been determined during cisplatin and ischemia-induced acute renal failure in the rat. Northern analysis of polyadenylated RNAs of kidney cortical tissue showed diminished renal preproEGF mRNA in rats injected wi...
journal_title:Kidney international
pub_type: 杂志文章
doi:10.1038/ki.1989.266
更新日期:1989-11-01 00:00:00