In vivo dynamic MRI tracking of rat T-cells labeled with superparamagnetic iron-oxide particles.

Abstract:

:Dynamic MRI tracking of rat T-cells in vivo is performed in rat testicles after labeling isolated rat T-cells in vitro with superparamagnetic dextran-coated iron-oxide particles, BMS180549. Tissue inflammation induced by the local injection of the calcium ionophore, A23187, is used to attract labeled T-cells. Gradient-echo MR images of rat testicles show a statistically significant decrease (4%) of the signal intensity in areas of injection of A23187 as early as 30 min after intravenous infusion of 2 x 10(8) labeled T-cells. The signal change reaches its maximum (6-7% decrease) at about 60-120 min after cell infusion. T2-mapping also shows a decrease of T2 in the areas with A23187. Image quantitation, which includes a chemical-shift effect, significantly enhances the sensitivity for detection of superparamagnetically labeled T-cells. Localization of labeled T-cells in rat testicles has been verified by fluorescence microscopy studies of T-cells co-labeled with a lipophilic fluorescent carbocyanine dye, 1,1-dioctadecyl-3,3,3',3'-tetramethyl-lindocarbocyanine perchlorate. These results represent the first successful demonstration of dynamic tracking of specific cells in vivo using MRI.

journal_name

Magn Reson Med

authors

Yeh TC,Zhang W,Ildstad ST,Ho C

doi

10.1002/mrm.1910330209

subject

Has Abstract

pub_date

1995-02-01 00:00:00

pages

200-8

issue

2

eissn

0740-3194

issn

1522-2594

journal_volume

33

pub_type

杂志文章
  • Proton NMR study of the state of water in fibrin gels, plasma, and blood clots.

    abstract::A proton NMR relaxation and pulsed field gradient self-diffusion study of water in fibrin gels, plasma, and blood clots has been performed with special emphasis on the effect of the sol-gel and shrinkage transitions. Deuteron NMR in fibrin gels was also studied to supplement the proton data. It is shown that a measure...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910140111

    authors: Blinc A,Lahajnar G,Blinc R,Zidansek A,Sepe A

    更新日期:1990-04-01 00:00:00

  • Free breathing lung T1 mapping using image registration in patients with idiopathic pulmonary fibrosis.

    abstract:PURPOSE:To assess the use of image registration for correcting respiratory motion in free breathing lung T1 mapping acquisition in patients with idiopathic pulmonary fibrosis (IPF). THEORY AND METHODS:The method presented used image registration to synthetic images during postprocessing to remove respiratory motion. S...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.28342

    authors: Saunders LC,Eaden JA,Bianchi SM,Swift AJ,Wild JM

    更新日期:2020-12-01 00:00:00

  • Simultaneous T(1) and B(1) (+) mapping using reference region variable flip angle imaging.

    abstract:PURPOSE:To present a new method that can simultaneously and efficiently measure T1 and B1 (+) maps using reference region variable flip angle (RR-VFA) imaging. METHODS:Assuming T1 relaxation time in a reference region such as fat is well characterized, and the reference region sufficiently covers smoothly varying B1 (...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24904

    authors: Sung K,Saranathan M,Daniel BL,Hargreaves BA

    更新日期:2013-10-01 00:00:00

  • Phase constrained encoding (PACE): a technique for MRI in large static field inhomogeneities.

    abstract::In spin echo imaging, magnetization is assigned to a location defined by its frequency of rotation. In the presence of a static magnetic field inhomogeneity, however, this location does not correspond to the true location of the magnetization. This paper describes a magnetic resonance imaging technique called phase co...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910330407

    authors: Kim JK,Plewes DB,Henkelman RM

    更新日期:1995-04-01 00:00:00

  • Multi-turn transmit coil to increase b1 efficiency in current source amplification.

    abstract:PURPOSE:A multi-turn transmit surface coil design was presented to improve B1 efficiency when used with current source amplification. METHODS:Three different coil designs driven by an on-coil current-mode class-D amplifier with current envelope feedback were tested on the benchtop and through imaging in a 1.5 T scanne...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24612

    authors: Gudino N,Griswold MA

    更新日期:2013-04-01 00:00:00

  • Multinuclear NMR investigations of probe construction materials at 4.7 T.

    abstract::Since background signal from NMR probes can overwhelm the signal from a sample, the NMR analysis of plastics or machinable ceramics is a prerequisite to their use in probe implementations. The contributions from a variety of materials to proton, fluorine, and carbon NMR spectra are presented. ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910130317

    authors: Babcock EE,Vaughan JT,Lesan B,Nunnally RL

    更新日期:1990-03-01 00:00:00

  • Regularized higher-order in vivo shimming.

    abstract::A regularized algorithm is presented for localized in vivo shimming. The technique uses first- (X,Y,Z), second- (Z(2), ZX, ZY, X(2)-Y(2), XY), and third-order (Z(3)) shim coils, and is robust when applied to arbitrarily-shaped, as well as off-center, regions of interest (ROIs). A single-shot spiral pulse sequence is u...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.10267

    authors: Kim DH,Adalsteinsson E,Glover GH,Spielman DM

    更新日期:2002-10-01 00:00:00

  • A 1H-NMR method for determining temperature in cell culture perfusion systems.

    abstract::This report describes a noninvasive 1H-NMR method for measuring absolute temperatures (+/- 0.2 degrees C) in biological samples and, in particular, in cell culture perfusion systems, utilizing the linear temperature dependence of the water chemical shift relative to the temperature-independent shift of one of the comp...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910290120

    authors: Lutz NW,Kuesel AC,Hull WE

    更新日期:1993-01-01 00:00:00

  • Magnetization transfer contrast-suppressed imaging of amide proton transfer and relayed nuclear overhauser enhancement chemical exchange saturation transfer effects in the human brain at 7T.

    abstract:PURPOSE:To use the variable delay multipulse (VDMP) chemical exchange saturation transfer (CEST) approach to obtain clean amide proton transfer (APT) and relayed Nuclear Overhauser enhancement (rNOE) CEST images in the human brain by suppressing the conventional magnetization transfer contrast (MTC) and reducing the di...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.25990

    authors: Xu X,Yadav NN,Zeng H,Jones CK,Zhou J,van Zijl PC,Xu J

    更新日期:2016-01-01 00:00:00

  • Coronary MR angiography using image-based respiratory motion compensation with inline correction and fixed gating efficiency.

    abstract:PURPOSE:The purpose of this study was to evaluate a new inline motion compensation approach called image-based navigation with Constant Respiratory efficiency UsIng Single End-expiratory threshold (iNAV-CRUISE) for coronary MR angiography (CMRA). METHODS:The CRUISE gating technique was combined with iNAV motion correc...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26678

    authors: Henningsson M,Smink J,van Ensbergen G,Botnar R

    更新日期:2018-01-01 00:00:00

  • Sources of functional apparent diffusion coefficient changes investigated by diffusion-weighted spin-echo fMRI.

    abstract::The mechanism behind previously observed changes in the apparent diffusion coefficient (ADC) during brain activation is not well understood. Therefore, we investigated the signal source and spatial specificity of functional magnetic resonance imaging (fMRI) ADC changes systematically in the visual cortex of cats using...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.21074

    authors: Jin T,Zhao F,Kim SG

    更新日期:2006-12-01 00:00:00

  • Increased anatomical detail by in vitro MR microscopy with a modified Golgi impregnation method.

    abstract::Golgi impregnation is unique in its ability to display the dendritic trees and axons of large numbers of individual neurons by histology. Here we apply magnetic resonance microscopy to visualize the neuroanatomy of animal models by combining histologic fixation chemistry with paramagnetic contrast agents. Although the...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.22322

    authors: Zhang X,Bearer EL,Perles-Barbacaru AT,Jacobs RE

    更新日期:2010-05-01 00:00:00

  • Calculation of signal intensities in hybrid sequences for fast NMR imaging.

    abstract::A number of techniques that recently have been used for fast NMR-imaging are based on a hybrid sequence of echo planar imaging (EPI) and FLASH imaging: after each NMR excitation several k-space lines are measured. The complete k-space is covered by performance of several excitations. It has been observed that there is...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910340328

    authors: Deichmann R,Adolf H,Nöth U,Kuchenbrod E,Schwarzbauer C,Haase A

    更新日期:1995-09-01 00:00:00

  • Partial-volume effect on ischemic tissue-fate delineation using quantitative perfusion and diffusion imaging on a rat stroke model.

    abstract::Partial-volume effects (PVE) in stroke imaging could hinder proper delineation of normal, ischemic, and at-risk tissues. Cerebral-blood-flow (CBF) and apparent diffusion coefficient (ADC) were measured at high and low resolution (HR = 128 x 128, LR = 64 x 64) in focal ischemia in rats during the acute phase. The data ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.20299

    authors: Ren H,Shen Q,Bardutzky J,Fisher M,Duong TQ

    更新日期:2004-12-01 00:00:00

  • Quantitative fat and R2* mapping in vivo to measure lipid-rich necrotic core and intraplaque hemorrhage in carotid atherosclerosis.

    abstract:PURPOSE:The aim of this work was to quantify the extent of lipid-rich necrotic core (LRNC) and intraplaque hemorrhage (IPH) in atherosclerotic plaques. METHODS:Patients scheduled for carotid endarterectomy underwent four-point Dixon and T1-weighted magnetic resonance imaging (MRI) at 3 Tesla. Fat and R2* maps were gen...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26359

    authors: Koppal S,Warntjes M,Swann J,Dyverfeldt P,Kihlberg J,Moreno R,Magee D,Roberts N,Zachrisson H,Forssell C,Länne T,Treanor D,de Muinck ED

    更新日期:2017-07-01 00:00:00

  • Proton magnetic resonance spectroscopy of Sjögren-Larsson syndrome heterozygotes.

    abstract::A deficit of fatty alcohol:NAD+ oxidoreductase complex (FAO) activity has been detected in patients with the Sjögren-Larsson syndrome (SLS). A moderate decrease in FAO activity has also been reported in heterozygote SLS subjects. Abnormal peaks were detected with proton magnetic resonance spectroscopy (1H-MRS) in homo...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1146

    authors: Kaminaga T,Mano T,Ono J,Kusuoka H,Nakamura H,Nishimura T

    更新日期:2001-06-01 00:00:00

  • Perfusion mapping with multiecho multishot parallel imaging EPI.

    abstract::Echo-planar imaging (EPI) is the standard technique for dynamic susceptibility-contrast (DSC) perfusion MRI. However, EPI suffers from well-known geometric distortions, which can be reduced by increasing the k-space phase velocity. Moreover, the long echo times (TEs) used in DSC lead to signal saturation of the arteri...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.21255

    authors: Newbould RD,Skare ST,Jochimsen TH,Alley MT,Moseley ME,Albers GW,Bammer R

    更新日期:2007-07-01 00:00:00

  • Quantitative diffusion imaging in implanted human breast tumors.

    abstract::Quantitative diffusion measurements were performed in tumors arising from inoculation of nude mice with two human breast cancer cell lines (MCF7 and T47D) to evaluate the specificity of this technique for characterizing solid tumors. ADC maps were compared to histology and correlated well with gross tumor morphology. ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910370417

    authors: Maier CF,Paran Y,Bendel P,Rutt BK,Degani H

    更新日期:1997-04-01 00:00:00

  • Biexponential longitudinal relaxation in white matter: Characterization and impact on T1 mapping with IR-FSE and MP2RAGE.

    abstract:PURPOSE:Magnetization transfer in white matter (WM) causes biexponential relaxation, but most quantitative T1 measurements fit data assuming monoexponential relaxation. The resulting monoexponential T1 estimate varies based on scan parameters and represents a source of variation between studies, especially at high fiel...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.25729

    authors: Rioux JA,Levesque IR,Rutt BK

    更新日期:2016-06-01 00:00:00

  • Perfusion-weighted imaging of interictal hypoperfusion in temporal lobe epilepsy using FAIR-HASTE: comparison with H(2)(15)O PET measurements.

    abstract::To detect perfusion abnormalities in areas of high magnetic susceptibility in the brain, an arterial spin-labeling MRI technique utilizing flow-sensitive alternating inversion recovery (FAIR) and half-Fourier single shot turbo spin-echo (HASTE) for spin preparation and image acquisition, respectively, was developed. I...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/1522-2594(200103)45:3<431::aid-mrm1056>3.0

    authors: Liu HL,Kochunov P,Hou J,Pu Y,Mahankali S,Feng CM,Yee SH,Wan YL,Fox PT,Gao JH

    更新日期:2001-03-01 00:00:00

  • Phosphorus-31 magnetic resonance spectroscopy in humans by spectroscopic imaging: localized spectroscopy and metabolite imaging.

    abstract::In in vivo phosphorus magnetic resonance spectroscopy (MRS), spectroscopic imaging (SI) can be used as a flexible localization technique, producing spectra from multiple volumes in a single examination. Presented here are phosphorus SI studies of human organs in which a selective-volume SI reconstruction was used rath...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910120302

    authors: Twieg DB,Meyerhoff DJ,Hubesch B,Roth K,Sappey-Marinier D,Boska MD,Gober JR,Schaefer S,Weiner MW

    更新日期:1989-12-01 00:00:00

  • Parallel reconstruction using null operations.

    abstract::A novel iterative k-space data-driven technique, namely parallel reconstruction using null operations (PRUNO), is presented for parallel imaging reconstruction. In PRUNO, both data calibration and image reconstruction are formulated into linear algebra problems based on a generalized system model. An optimal data cali...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.22899

    authors: Zhang J,Liu C,Moseley ME

    更新日期:2011-11-01 00:00:00

  • Volumetric navigators for real-time motion correction in diffusion tensor imaging.

    abstract::Prospective motion correction methods using an optical system, diffusion-weighted prospective acquisition correction, or a free induction decay navigator have recently been applied to correct for motion in diffusion tensor imaging. These methods have some limitations and drawbacks. This article describes a novel techn...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.23314

    authors: Alhamud A,Tisdall MD,Hess AT,Hasan KM,Meintjes EM,van der Kouwe AJ

    更新日期:2012-10-01 00:00:00

  • Intimate combination of low- and high-resolution image data: I. Real-space PET and (1)H(2)O MRI, PETAMRI.

    abstract::Two different types of (co-registered) images of the same slice of tissue will generally have different spatial resolutions. The judicious pixel-by-pixel combination of their data can be accomplished to yield a single image exhibiting properties of both. Here, axial (18)FDG PET and (1)H(2)O MR images of the human brai...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1522-2594(199908)42:2<345::aid-mrm17

    authors: Sammi MK,Felder CA,Fowler JS,Lee JH,Levy AV,Li X,Logan J,Pályka I,Rooney WD,Volkow ND,Wang GJ,Springer CS Jr

    更新日期:1999-08-01 00:00:00

  • Fast slice-selective radio-frequency excitation pulses for mitigating B+1 inhomogeneity in the human brain at 7 Tesla.

    abstract::A novel radio-frequency (RF) pulse design algorithm is presented that generates fast slice-selective excitation pulses that mitigate B+1 inhomogeneity present in the human brain at high field. The method is provided an estimate of the B+1 field in an axial slice of the brain and then optimizes the placement of sinc-li...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.21585

    authors: Zelinski AC,Wald LL,Setsompop K,Alagappan V,Gagoski BA,Goyal VK,Adalsteinsson E

    更新日期:2008-06-01 00:00:00

  • Volumetric analysis of white matter, gray matter, and CSF using fractional volume analysis.

    abstract::Quantitative cerebral tissue volumes may be useful for an objective assessment of pathological changes in brain. Accurate determination of tissue volumes is complicated, however, by the partial volume averaging (PVA) effect. We have, therefore, developed a new pulse sequence that minimizes the PVA through the use of i...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910390614

    authors: Bedell BJ,Narayana PA

    更新日期:1998-06-01 00:00:00

  • Fast diffusion tensor imaging and tractography of the whole cervical spinal cord using point spread function corrected echo planar imaging.

    abstract::Diffusion tensor imaging has been used in a number of spinal cord studies, but severe distortions caused by susceptibility induced field inhomogeneities limit its applicability to investigate small volumes within acceptable acquisition times. A way to evaluate image distortions is to map the point spread function of t...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24235

    authors: Lundell H,Barthelemy D,Biering-Sørensen F,Cohen-Adad J,Nielsen JB,Dyrby TB

    更新日期:2013-01-01 00:00:00

  • Model of the human vasculature for studying the influence of contrast injection speed on cerebral perfusion MRI.

    abstract::Simulations of dynamic susceptibility contrast (DSC) MRI are frequently performed by assuming a certain shape for the input function and the microvascular response function. However, to investigate the influence of parameters that will affect the shape of the input function, a more complex model of the human vasculatu...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.10567

    authors: van Osch MJ,Vonken EJ,Wu O,Viergever MA,van der Grond J,Bakker CJ

    更新日期:2003-09-01 00:00:00

  • 31P NMR investigation of energy metabolism in perifused MMQ cells.

    abstract::The MMQ cell line is a unique prolactin-secreting rat pituitary cell line. MMQ cells entrapped in agarose gel threads are metabolically active, as determined by the uptake and phosphorylation of creatine and the maintenance of high energy phosphates for over 15 h. Forskolin activates the catalytic subunit of adenylyl ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910320507

    authors: Goger MJ,Login IS,Fernandez EJ,Grisham CM

    更新日期:1994-11-01 00:00:00

  • A novel phase-unwrapping method based on pixel clustering and local surface fitting with application to Dixon water-fat MRI.

    abstract:PURPOSE:To develop and evaluate a novel 2D phase-unwrapping method that works robustly in the presence of severe noise, rapid phase changes, and disconnected regions. THEORY AND METHODS:The MR phase map usually varies rapidly in regions adjacent to wraps. In contrast, the phasors can vary slowly, especially in regions...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26647

    authors: Cheng J,Mei Y,Liu B,Guan J,Liu X,Wu EX,Feng Q,Chen W,Feng Y

    更新日期:2018-01-01 00:00:00