Abstract:
OBJECTIVES:To investigate the inhibition of PANC-1 pancreatic cancer cell growth by cucurmosin (CUS) and its possible mechanism. METHODS:We observed the inhibition of PANC-1 cell growth by sulforhodamine B and colony-forming experiments in vitro and established nonobese diabetic/severe combined immunodeficiency mouse subcutaneous tumor models in vivo. We used Western blot to analyze protein levels related to apoptosis and epidermal growth factor receptor (EGFR) signaling pathways after drug intervention, whereas the messenger RNA expression of EGFR was analyzed by quantitative real-time polymerase chain reaction. RESULTS:Sulforhodamine B and colony-forming experiments indicated that CUS inhibited PANC-1 cell proliferation in a dose- and time-dependent manner. A stronger inhibitory effect was observed when CUS was combined with gefitinib. The subcutaneous tumor growth was also inhibited. Western blot showed that all the examined proteins decreased, except for 4E-BP1 and the active fragments of caspase 3 and caspase 9 increased. Epidermal growth factor receptor expression did not change significantly in quantitative real-time polymerase chain reaction. CONCLUSIONS:Cucurmosin can strongly inhibit the growth of PANC-1 cells in vitro and in vivo. Cucurmosin can down-regulate EGFR protein expression, but not at the messenger RNA level. Cucurmosin can also inhibit the ras/raf and phosphatidylinositol 3-kinase/Akt downstream signaling pathways and enhance the sensitivity of the EGFR-targeted drug gefitinib.
journal_name
Pancreasjournal_title
Pancreasauthors
Wang C,Yang A,Zhang B,Yin Q,Huang H,Chen M,Xie Jdoi
10.1097/MPA.0000000000000087subject
Has Abstractpub_date
2014-03-01 00:00:00pages
291-7issue
2eissn
0885-3177issn
1536-4828pii
00006676-201403000-00019journal_volume
43pub_type
杂志文章相关文献
PANCREAS文献大全abstract::Sarcophytol A (SaA), a cembrane-type diterpene isolated from the marine soft coral Sarcophyton glaucum showed anticancer and cancer preventive effects in two different experiments. Growth of transplanted human pancreatic cancer cells (MIA paca-2, 1 x 10(7)) in nude mice (BALB/C 4W female) (Experiment 1) and pancreatic...
journal_title:Pancreas
pub_type: 杂志文章
doi:
更新日期:1994-07-01 00:00:00
abstract::This review discusses the current imaging modalities for the diagnosis and staging of solid and cystic pancreatic lesions and for the assessment of acute and chronic pancreatitis, and the future role of emerging technologies in the management of pancreatic diseases. Multidetector row spiral computed tomography is supe...
journal_title:Pancreas
pub_type: 杂志文章,评审
doi:10.1097/01.mpa.0000227912.71202.2c
更新日期:2006-10-01 00:00:00
abstract::With a nitrosamine induced hamster pancreatic cancer cell line (HaP-T1), survival time and metastatic rates were compared between orthotopic cell implantation (OCI; n = 5) and orthotopic tissue implantation (OTI; n = 5) models. All the tumors were palpable (100% tumor take) after 1 week in both groups. Hamsters in the...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200003000-00007
更新日期:2000-03-01 00:00:00
abstract:OBJECTIVES:Tissue desmoplasia occurs in a number of disease states, but its molecular basis is poorly understood. To determine which genes are overexpressed in cells contained within the desmoplastic stroma of pancreatic adenocarcinoma and chronic pancreatitis, we undertook genetic profiling of microdissected tissue sa...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200411000-00003
更新日期:2004-11-01 00:00:00
abstract::Relative adrenal insufficiency is common in patients with severe sepsis and septic shock. We describe three patients with severe acute pancreatitis who developed signs suggestive of adrenal insufficiency during the early phase of the disease. Clinical features and possible causes of adrenal insufficiency are discussed...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200310000-00009
更新日期:2003-10-01 00:00:00
abstract:OBJECTIVES:Nuclear factor κB (NF-κB) plays an intrinsic role in promoting growth, angiogenesis, and metastasis in pancreatic cancer (PC) and serves as a mechanism underlying therapeutic resistance. Accordingly, we investigated the efficacy of bioactive phytochemicals in inhibiting radiotherapy (RT)-induced NF-κB activi...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e31821f677d
更新日期:2011-10-01 00:00:00
abstract::Wheat amylase inhibitor (WAI) was given to growing rats to determine whether chronic inhibition of intraluminal amylase activity alters pancreatic growth, pancreatic enzyme composition, and secretory responsiveness to cholecystokinin octapeptide (CCK-OP) and carbachol. For 21 days 13 rats were fed amylase inhibitor (A...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199807000-00006
更新日期:1998-07-01 00:00:00
abstract:OBJECTIVES:Adhesion molecules are involved in the inflammatory response during acute pancreatitis (AP). We investigated the effect of dexamethasone (Dx) on intercellular adhesion molecule 1 (ICAM-1) expression during AP and its consequences on leukocyte recruitment and pancreatic damage. METHODS:Acute pancreatitis was...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3181da0f3e
更新日期:2010-10-01 00:00:00
abstract:OBJECTIVES:Pancreatic carcinoma etiology and molecular pathogenesis is weakly understood. According to the assumption that genetic variation in carcinogen metabolism further modifies the risk of exposure-related cancers, an association of functional polymorphisms in oxidative stress-modifying genes superoxide dismutase...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3181f74ad7
更新日期:2011-01-01 00:00:00
abstract::Islet amyloid polypeptide (IAPP) is a 37-amino acid residue polypeptide, originally isolated from the pancreatic amyloid deposits of patients with type II diabetes mellitus. Subsequently, IAPP was found to be colocalised with insulin in beta-cell secretory granules of the normal mammalian pancreas. Recently, IAPP has ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199303000-00019
更新日期:1993-03-01 00:00:00
abstract:OBJECTIVES:Pancreatic functions were determined in a Ki-ras-induced actin-interacting protein (KRAP)-deficient (-/-) mouse mutant. METHODS:Pancreatic enzyme, protein, and DNA contents were measured, and histological examinations were conducted. The mixture of bile-pancreatic juice was collected, and amylase and bile a...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3181f66c22
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVES:Nitric oxide (NO) is a potent nonadrenergic, noncholinergic mediator of gastrointestinal smooth muscle. We aimed to investigate the effects of new NO/cyclic guanosine monophosphate (cGMP) pathway-affecting agents at the sheep sphincter of Oddi (SO) in vitro. METHODS:Sheep SO rings were mounted in organ bath...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3181d3645b
更新日期:2010-08-01 00:00:00
abstract::The effects of manganese (Mn2+) on phosphorylation activity in the rat pancreas were examined in an in vitro phosphorylation assay and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of endogenous proteins. Several histones were phosphorylated in the presence of pancreatic supernatant obtained ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199009000-00015
更新日期:1990-09-01 00:00:00
abstract:OBJECTIVES:In reference to our earlier publication, laboratory tests that reflect severe intravascular volume depletion can be used for predicting the severity of acute pancreatitis (AP). The aim of the study was to assess whether urinary level of neutrophil gelatinase-associated lipocalin (NGAL) could represent a usef...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000282
更新日期:2015-04-01 00:00:00
abstract:OBJECTIVES:Most of the acetaldehyde, a recognized animal carcinogen, generated during alcohol metabolism is eliminated by liver mitochondrial aldehyde dehydrogenase 2 (ALDH2). More than 40% of Japanese have the inactive form of ALDH2, and inactive ALDH2 is a risk factor for multiple cancers of the esophagus as well as ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/01.mpa.0000147084.70125.41
更新日期:2005-03-01 00:00:00
abstract:INTRODUCTION:Hypercytokinemia is known to occur in severe acute pancreatitis, suggesting that the production, deposition, and degradation of the extracellular matrix (ECM) occur actively as a result of the actions of the complicated cytokine network. AIMS AND METHODOLOGY:To examine the influence of cytokines and growt...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200303000-00008
更新日期:2003-03-01 00:00:00
abstract::OBJECTIVES: The purpose of this study was to determine whether the choice for performance of endoscopic retrograde pancreatography (ERP) could be tailored to findings on computed tomography (CT) in patients with suspected autoimmune pancreatitis (AIP). METHODS: Eighty-four AIP patients and 73 pathology-proven pancreat...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e31823a509b
更新日期:2012-05-01 00:00:00
abstract:OBJECTIVE:Opioid therapy for pain in chronic pancreatitis (CP) is associated with tolerance and possibly opioid-induced hyperalgesia. We thus examined opioid use and pain rating in CP patients. METHODS:Medical records of patients with established CP treated at the University of Pittsburgh Medical Center's Digestive Di...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e318224056f
更新日期:2012-03-01 00:00:00
abstract::The glycolytic inhibitor, 2-deoxy-D-glucose (2-DG), inhibits growth of some cancers. alpha-Difluoromethylornithine (DFMO) is an irreversible inhibitor of ornithine decarboxylase, the rate-limiting enzyme in polyamine biosynthesis. We and others have previously shown that DFMO inhibits cancer growth in a number of mode...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-198902000-00006
更新日期:1989-01-01 00:00:00
abstract::The mechanism of tissue alteration in chronic pancreatitis (CP) is still unclear. Different hypotheses have been discussed, including increasing oxidant stress in the acinar cells, often as a result of exposure to xenobiotics. To evaluate the role of oxidative stress in CP, the authors investigated the expression of t...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200105000-00009
更新日期:2001-05-01 00:00:00
abstract:OBJECTIVES:In the hamster model, pancreatic ductal adenocarcinoma develops after treatment with N-nitrosobis-(2-oxopropyl)amino (BOP). In this model, Langerhans islets play a central role in carcinogenesis. In contrast, treatment with BOP in rats and mice did not result in cancer development. We investigated whether pa...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3182016a08
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVES:Based mainly on animal models, 2 lesions have been suggested as possible precursors of pancreatic ductal adenocarcinoma (PDAC): pancreatic intraepithelial neoplasia (PanIN) and tubular complexes (TCs). The aim of the study was to find support for either of the 2 models through the analysis of a large panel o...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/mpa.0b013e31805d0190
更新日期:2007-10-01 00:00:00
abstract::The effect of a potent and long-acting bradykinin B2-receptor antagonist (HOE140) on acute pancreatitis induced by retrograde infusion of trypsin and taurocholate into the pancreatic duct was studied in rats. HOE140 was administered subcutaneously immediately before and 3 h after the induction of pancreatitis and the ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199610000-00011
更新日期:1996-10-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to identify differentially expressed proteins among various stages of pancreatic ductal adenocarcinoma (PDAC) by shotgun proteomics using nano-liquid chromatography coupled tandem mass spectrometry and stable isotope dimethyl labeling. METHODS:Differentially expressed proteins were...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000388
更新日期:2016-01-01 00:00:00
abstract::This study compared diet, type of alcoholism, and smoking in three groups of alcoholic men, with chronic pancreatitis (n = 56), with histological cirrhosis (n = 50), and without pancreatitis or cirrhosis (controls; n = 50) by a multidimensional analysis. Only patients in whom the first symptom of pancreatitis or cirrh...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199504000-00003
更新日期:1995-04-01 00:00:00
abstract:OBJECTIVES:The objective of this study was to evaluate the relevance of defining borderline resectable (BR) pancreatic cancer as a distinct entity in the treatment scheme of pancreatic cancer as proposed by the National Comprehensive Cancer Network. METHODS:Among 375 patients with pancreatic cancer, 137 patients were ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e31827b2d7c
更新日期:2013-08-01 00:00:00
abstract:OBJECTIVES:Mammalian targets of rapamycin inhibitors (mTORi) are considered second-line immunosuppression agents because of associated increases in rejection and impaired wound healing. Recent reports indicate mTORi have been linked to improved survival, decreased inflammatory response in pancreatitis, and antiprolifer...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001322
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:The aim of the study was to observe the role of nucleotide-binding and oligomerization domain (NOD)-like receptors (NLR) in intestinal injury of severe acute pancreatitis (SAP) in rats. METHODS:Severe acute pancreatitis was induced by retrograde infusion of sodium taurocholate into the biliopancreatic duct....
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000977
更新日期:2018-02-01 00:00:00
abstract::The 3 International Conference for Cancer Metabolism and Therapy was successfully held at the South Hospital Conference Center of Shanghai First People's Hospital, nearly 200 international experts from the field of cancer metabolism and therapy and about two thousand local scientists attended the conference. The confe...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001482
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVES:We examined pancreatic cancer risk in association with hormonal and reproductive factors in a prospective cohort study of 89,835 Canadian women, aged 40-59 at recruitment, who were enrolled in the National Breast Screening Study (NBSS). METHODS:Linkages to national cancer and mortality databases yielded dat...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/01.mpa.0000160301.59319.ba
更新日期:2005-05-01 00:00:00