Amplification of bacterial DNA using highly conserved sequences: automated analysis and potential for molecular triage of sepsis.

Abstract:

OBJECTIVE:The clinical diagnosis of sepsis remains difficult, particularly in the young child, and would be improved by a rapid and reliable method for identification of bacteria in blood and other body fluids. Polymerase chain reaction (PCR) amplification of highly conserved DNA sequences found in all bacteria would permit fast and sensitive determination of the presence of bacteria in clinical specimens. METHODOLOGY:A primer pair for highly conserved regions of bacterial DNA encoding 16S ribosomal RNA (rDNA) was utilized for PCR amplification. PCR products were analyzed by gel electrophoresis, and, after modification of the primers, by an automated 96-well plate reader. RESULTS:rDNA was amplified from 12 different species of bacteria, including Gram-negative and -positive organisms. No signal was observed when total human DNA was used as template. Colorimetric analysis of amplified sequences using a 96-well format was also successful. CONCLUSIONS:We conclude that a single primer pair designed to anneal to a highly conserved region of bacterial DNA can amplify DNA specimens from a variety of different bacteria, while not amplifying human DNA. Such a molecular genetics approach can be fully automated with existing robotic technology. Because of speed, sensitivity, and cost, molecular triage of patients with signs and symptoms of possible bacterial infection will decrease morbidity and mortality among those with unrecognized bacteremia who are managed as outpatients, and will dramatically reduce hospital expenses from individuals who are admitted and are not bacteremic.

journal_name

Pediatrics

journal_title

Pediatrics

authors

McCabe KM,Khan G,Zhang YH,Mason EO,McCabe ER

subject

Has Abstract

pub_date

1995-02-01 00:00:00

pages

165-9

issue

2

eissn

0031-4005

issn

1098-4275

journal_volume

95

pub_type

杂志文章
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    abstract::A growing body of scientific literature demonstrates that children who grow up with 1 or 2 gay and/or lesbian parents fare as well in emotional, cognitive, social, and sexual functioning as do children whose parents are heterosexual. Children's optimal development seems to be influenced more by the nature of the relat...

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    pub_type:

    doi:10.1542/peds.109.2.341

    authors: Perrin EC,Committee on Psychosocial Aspects of Child and Family Health.

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  • The Cockayne syndrome: an evaluation of hypertension and studies of renal pathology.

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    authors: Higginbottom MC,Griswold WR,Jones KL,Vasquez MD,Mendoza SA,Wilson CB

    更新日期:1979-12-01 00:00:00

  • NICU Dialects: Understanding Norwegian Practice Variation.

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    doi:10.1542/peds.2018-0478E

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    更新日期:2018-09-01 00:00:00

  • Effect of maternal lead burden on infant weight and weight gain at one month of age among breastfed infants.

    abstract:INTRODUCTION:Transfer of lead from bone to the bloodstream increases during lactation. However, the effect of maternal lead burden on growth in breastfed newborns is still unknown. This study examined early postnatal growth in a cohort of healthy breastfed newborns in relation to maternal bone lead burden. METHODS:Lea...

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    doi:10.1542/peds.107.5.1016

    authors: Sanín LH,González-Cossío T,Romieu I,Peterson KE,Ruíz S,Palazuelos E,Hernández-Avila M,Hu H

    更新日期:2001-05-01 00:00:00

  • Language ability in children with permanent hearing impairment: the influence of early management and family participation.

    abstract:OBJECTIVE:The goal was to examine the relationships between management after confirmation, family participation, and speech and language outcomes in the same group of children with permanent childhood hearing impairment. METHODS:Speech, oral language, and nonverbal abilities, expressed as z scores and adjusted in a re...

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    doi:10.1542/peds.2006-2116

    authors: Watkin P,McCann D,Law C,Mullee M,Petrou S,Stevenson J,Worsfold S,Yuen HM,Kennedy C

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  • The efficacy of motivational interviewing versus brief advice for adolescent smoking behavior change.

    abstract:OBJECTIVE:In this study we sought to evaluate the efficacy of motivational interviewing (MI) compared with structured brief advice (SBA) for adolescent smoking behavior change. METHODS:Participants (N=355) were randomly assigned to 5 sessions of either MI or SBA. The primary outcomes were attempts to reduce and to qui...

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    doi:10.1542/peds.2010-2174

    authors: Audrain-McGovern J,Stevens S,Murray PJ,Kinsman S,Zuckoff A,Pletcher J,Moss D,Baumritter A,Kalkhuis-Beam S,Carlson E,Rodriguez D,Wileyto EP

    更新日期:2011-07-01 00:00:00

  • Day-by-day postnatal survival in very low birth weight infants.

    abstract:OBJECTIVES:Postnatal survival rates of very low birth weight (VLBW) infants are well established for each birth weight or gestational age category. These figures do not differentiate viable infants who survive the first few days of life from extremely immature ones who die shortly after birth. This study aimed to devel...

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    pub_type: 杂志文章

    doi:10.1542/peds.2009-2810

    authors: Mohamed MA,Nada A,Aly H

    更新日期:2010-08-01 00:00:00

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    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2007-1146

    authors: Khoshoo V,Edell D,Thompson A,Rubin M

    更新日期:2007-11-01 00:00:00

  • Neurofibromatosis type 1 revisited.

    abstract::Neurofibromatosis type 1 (NF1) is an autosomal dominant condition with a worldwide incidence of approximately 1 per 2500 to 3000 individuals. Caused by a germ-line-inactivating mutation in the NF1 gene on chromosome 17, the disease is associated with increased morbidity and mortality. In the past several years, signif...

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    pub_type: 杂志文章,评审

    doi:10.1542/peds.2007-3204

    authors: Williams VC,Lucas J,Babcock MA,Gutmann DH,Korf B,Maria BL

    更新日期:2009-01-01 00:00:00

  • Teaching clinical decision-making to pediatric residents in an era of managed care.

    abstract::The growth of managed care has brought a new focus on physician competency in the appropriate use of resources to help patients. The community of pediatric educators must improve residency curricula and teaching methodologies to ensure that graduates of their programs can effectively and efficiently meet the needs of ...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:

    authors: Chessare JB

    更新日期:1998-04-01 00:00:00

  • Systematic evidence review of newborn screening and treatment of severe combined immunodeficiency.

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    journal_title:Pediatrics

    pub_type: 杂志文章,评审

    doi:10.1542/peds.2009-1567

    authors: Lipstein EA,Vorono S,Browning MF,Green NS,Kemper AR,Knapp AA,Prosser LA,Perrin JM

    更新日期:2010-05-01 00:00:00

  • Pediatric patient safety in hospitals: a national picture in 2000.

    abstract:OBJECTIVE:To describe potential patient safety events for hospitalized children, examine associated factors, and explore impacts of safety events. METHODS:The newly released Patient Safety Indicators (PSIs), developed by researchers at the Agency for Healthcare Research and Quality to identify potential in-hospital pa...

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    authors: Miller MR,Zhan C

    更新日期:2004-06-01 00:00:00

  • Summary proceedings from the cardiology group on cardiovascular instability in preterm infants.

    abstract::The appropriate determination of adequate tissue perfusion and the best approach to treatment of perceived abnormalities in blood pressure in the neonate remain controversial. There is no consensus regarding the actual definition of hypotension in the neonate or how best to raise perceived low blood pressure. In addit...

    journal_title:Pediatrics

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    authors: Short BL,Van Meurs K,Evans JR,Cardiology Group.

    更新日期:2006-03-01 00:00:00

  • Pretransport and posttransport characteristics and outcomes of neonates who were admitted to a cardiac intensive care unit.

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    doi:10.1542/peds.2006-0719

    authors: Yeager SB,Horbar JD,Greco KM,Duff J,Thiagarajan RR,Laussen PC

    更新日期:2006-09-01 00:00:00

  • Partial liquid ventilation in critically ill infants receiving extracorporeal life support. Philadelphia Liquid Ventilation Consortium.

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    doi:10.1542/peds.99.1.e2

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  • Aspects of communication between patients and doctors: an analysis of the discourse in medical interviews.

    abstract::The transcripts of tape-recorded interviews of pediatric endocrinologists and patients with Turner's syndrome and their parents were reviewed in consultation with child psychoanalysts in order to evaluate certain aspects of communication between patients and doctors. The transcripts showed that the doctors often talke...

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    authors: Raimbault G,Cachin O,Limal JM,Eliacheff C,Rappaport R

    更新日期:1975-03-01 00:00:00

  • The Diagnosis of Polycystic Ovary Syndrome in Adolescents.

    abstract::Consensus has recently been reached by international pediatric subspecialty societies that otherwise unexplained persistent hyperandrogenic anovulation using age- and stage-appropriate standards are appropriate diagnostic criteria for polycystic ovary syndrome (PCOS) in adolescents. The purpose of this review is to su...

    journal_title:Pediatrics

    pub_type: 杂志文章,评审

    doi:10.1542/peds.2015-1430

    authors: Rosenfield RL

    更新日期:2015-12-01 00:00:00

  • Issues in estimating the prevalence of fetal alcohol syndrome: examination of 2 counties in New York State.

    abstract:OBJECTIVE:Two demographically similar counties included in the New York Fetal Alcohol Syndrome Surveillance Network had very different prevalence rates. This study examined the components of the surveillance in an attempt to discover the reasons for this discrepancy. METHODS:Erie County and Monroe County were the 2 mo...

    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:10.1542/peds.2006-0610

    authors: Druschel CM,Fox DJ

    更新日期:2007-02-01 00:00:00

  • Annual summary of vital statistics--1982.

    abstract::Data for this article, as in previous reports, are drawn principally from the Monthly Vital Statistics Report, published by the National Center for Health Statistics. The international data come from the Demographic Yearbook and the quarterly Population and Vital Statistics Report, both published by the Statistical Of...

    journal_title:Pediatrics

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    更新日期:1983-12-01 00:00:00

  • Implementation of a Standardized Approach to Improve the Pediatric Discharge Medication Process.

    abstract:BACKGROUND AND OBJECTIVES:The pediatric inpatient discharge medication process is complicated, and caregivers have difficulty managing instructions. Authors of few studies evaluate systematic processes for ensuring quality in these care transitions. We aimed to improve caregiver medication management and understanding ...

    journal_title:Pediatrics

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    doi:10.1542/peds.2019-2711

    authors: Philips K,Zhou R,Lee DS,Marrese C,Nazif J,Browne C,Sinnett M,Tuckman S,Modi A,Rinke ML

    更新日期:2021-02-01 00:00:00

  • Trends in environmentally related childhood illnesses.

    abstract::Tracking incidence or prevalence of diseases and using that information to target interventions is a well-established strategy for improving public health. The need to track environmentally mediated chronic diseases is increasingly recognized. Trends in childhood illnesses are 1 element of a framework for children's e...

    journal_title:Pediatrics

    pub_type: 杂志文章,评审

    doi:

    authors: Woodruff TJ,Axelrad DA,Kyle AD,Nweke O,Miller GG,Hurley BJ

    更新日期:2004-04-01 00:00:00

  • Trends in multiple births conceived using assisted reproductive technology, United States, 1997-2000.

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    journal_title:Pediatrics

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    doi:

    authors: Reynolds MA,Schieve LA,Martin JA,Jeng G,Macaluso M

    更新日期:2003-05-01 00:00:00

  • Rape prevention through empowerment of adolescent girls.

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    journal_title:Pediatrics

    pub_type: 杂志文章,随机对照试验

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    authors: Sarnquist C,Omondi B,Sinclair J,Gitau C,Paiva L,Mulinge M,Cornfield DN,Maldonado Y

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  • A program to limit donor exposures to neonates undergoing extracorporeal membrane oxygenation.

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    journal_title:Pediatrics

    pub_type: 杂志文章

    doi:

    authors: Rosenberg EM,Chambers LA,Gunter JM,Good JA

    更新日期:1994-09-01 00:00:00

  • Decreasing hypothermia during delivery room stabilization of preterm neonates.

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    authors: Pinheiro JM,Furdon SA,Boynton S,Dugan R,Reu-Donlon C,Jensen S

    更新日期:2014-01-01 00:00:00

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    abstract::Paraduodenal hernias are the most common type of congenital internal hernia. Because of its overall rare incidence, this entity is often overlooked during initial assessment of the patient. Lack of specific diagnostic criteria also makes diagnosis exceedingly difficult, and the resulting diagnostic delays can lead to ...

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    更新日期:2015-04-01 00:00:00

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    更新日期:1986-06-01 00:00:00