Abstract:
:Human immunodeficiency virus infects different cell types including CD4+ lymphocytes and monocyte-derived macrophages (MDMs). We have examined the activity of the HIV-1 Tat inhibitors Ro-5-3335 and Ro-24-7429 in cultured human peripheral MDMs. Monocytes were isolated from HIV-seronegative donors by gradient centrifugation and plastic adherence. MDMs and unfractionated peripheral blood mononuclear cells (PBMCs) were infected with HIV Ba-L and then treated with drug either immediately (acute infection) or after 4 days (PBMCs) or 14 days (MDMs) (chronic infection). Inhibition of HIV replication by each drug was assessed by quantitation of HIV p24 antigen in culture supernatant using an enzyme immunoassay. In acutely infected MDMs, Ro-5-3335 (10 microM) and Ro-24-7429 (10 microM) resulted in 77% and 99% mean inhibition, respectively, of HIV replication with a clear dose response at lower concentrations; chronically infected MDMs were much less susceptible to these drugs, with both compounds inhibiting p24 antigen production by less than 50% at 10 microM. The drugs had no deleterious effect on cell viability at any concentration tested. In acutely infected PBMCs Ro-5-3335 and Ro-24-7429 resulted in 68% and 68.5% mean inhibition at 10 microM; when the compounds were added 4 days after infection inhibition was less than 50% compared with controls. Thus, the Tat inhibitors were effective in inhibiting acute HIV infection in MDMs but not in chronically infected cells, findings that differ from those of published studies using continuous lymphoblastoid cell lines.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Dunne AL,Siregar H,Mills J,Crowe SMdoi
10.1002/jlb.56.3.369subject
Has Abstractpub_date
1994-09-01 00:00:00pages
369-73issue
3eissn
0741-5400issn
1938-3673journal_volume
56pub_type
杂志文章abstract::The frequency of human immunodeficiency virus (HIV)-infected monocytes that spread on a model basement membrane was about twofold greater than that of an equal number of uninfected control cells through the initial 12 to 18 h of culture. By 24 h, virtually all HIV-infected and uninfected control cells spread on the ba...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.52.2.244
更新日期:1992-08-01 00:00:00
abstract::Natural killer (NK) cells arise from immature progenitors present in fetal tissues and adult bone marrow, but the factors responsible for driving the proliferation and differentiation of these progenitors are poorly understood. Mouse NK cells had previously been thought not to express interleukin (IL)-2Ralpha chains, ...
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abstract::Previously we had determined that neonatal neutrophils had decreased interaction with monolayers expressing P-selectin compared to adult cells. In this study we examined the function of neonatal P-selectin glycoprotein ligand-1 (PSGL-1). A rabbit polyclonal antibody directed against the amino terminus of human PSGL-1 ...
journal_title:Journal of leukocyte biology
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doi:10.1002/jlb.67.1.73
更新日期:2000-01-01 00:00:00
abstract::The effects on macrophage growth of seven phospholipids that are normally present in cell membranes were examined. Phosphatidylcholine and sphingomyelin which are the main phospholipid constituents of mammalian cell membranes, had no effect on growth of macrophages. Phosphatidylinositol also had little effect. On the ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.39.6.713
更新日期:1986-06-01 00:00:00
abstract::We have studied two key stages of CD4 development that serve as important checkpoints in determining whether a response will occur, how big it will be, and how long it will last. The response of naive CD4 cells is strictly regulated by the requirement for interaction of the potential precursor with an antigen-presenti...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1002/jlb.57.5.795
更新日期:1995-05-01 00:00:00
abstract::Intracellular pathogens, including Mycobacterium tuberculosis, obtain iron from the host for their survival. Ferroportin 1 (FPN1; SLC40A1) is the sole iron exporter from mammalian cells and is expressed in the duodenum and macrophages. In the present study, we show that FPN1 mRNA levels in the mouse macrophage cell li...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1107781
更新日期:2008-09-01 00:00:00
abstract::Sexual transmission is the primary route of HIV-1 infection, and DC subsets are thought to be involved in viral dissemination to T cells. In the genital mucosa, two main subsets of DCs are present: epithelial LCs capture and degrade HIV-1 through C-type lectin Langerin, whereas subepithelial DCs express DC-SIGN, which...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0809577
更新日期:2010-04-01 00:00:00
abstract::Chemokines play a crucial role in combating microbial infection by recruiting blood neutrophils to infected tissue. In mice, the chemokines Cxcl1/KC and Cxcl2/MIP2 fulfill this role. Cxcl1 and Cxcl2 exist as monomers and dimers, and exert their function by activating the Cxcr2 receptor and binding glycosaminoglycans (...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.3A0820-207R
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abstract::Mice with a naturally occurring Csfm(op)/Csfm(op) (op/op) gene mutation lack functional macrophage-colony stimulating factor (M-CSF) and are deficient of M-CSF-derived macrophages. They are severely monocytopenic, and their remaining M-CSF-independent macrophages were shown to differ in differentiation and distinct fu...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.12011003
更新日期:2003-05-01 00:00:00
abstract::Neutrophil's responses to G protein-coupled chemoattractants are highly dependent on store-operated calcium (Ca(2+)) entry (SOCE). Platelet-activating factor (PAF), a primary chemoattractant, simultaneously increases cytosolic-free Ca(2+), intracellular pH (pH(i)), ERK1/2, and Akt/protein kinase B (PKB) phosphorylatio...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0307196
更新日期:2007-11-01 00:00:00
abstract::Soluble mediators and inducible cell-surface and matrix-bound molecules coordinate the cascade of events giving rise to leukocyte emigration. Knowledge of the specific mechanisms underlying the attraction of cells into a local site, however, remains sketchy. In particular, it is unclear how chemoattractants cause rapi...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.58.4.438
更新日期:1995-10-01 00:00:00
abstract::The macrophage mannose receptor (MR) appears to play an important role in the binding and phagocytosis of several human pathogens, but its phagocytic property and signaling pathways have been poorly defined. The general strategy to explore such topics is to express the protein of interest in nonphagocytic cells, but i...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1204705
更新日期:2005-06-01 00:00:00
abstract::T helper cell type 1 (Th1)-polarizing cytokines are induced by Legionella pneumophila infection and are suppressed by pretreatment with marijuana cannabinoids (CB). Glucocorticoids and prostaglandin E2(PGE2) are also reported to suppress Th1 polarization and are induced by Delta9-tetrahydrocannabinol (THC), so their r...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0304184
更新日期:2004-10-01 00:00:00
abstract::We show that interleukin-18 is constitutively produced by dendritic cells; synthesis and secretion are poorly affected by maturative stimuli. Challenge of dendritic cells with autologous anti-tetanus toxoid T lymphocytes results in a secretory switch, with induction of secretion of biologically active interleukin-18 a...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Constitutive expression of cell-associated, but not secreted, interleukin-1alpha (IL-1alpha) by oncogene-transformed fibrosarcoma cells induced regressing tumors in mice, a phenomenon that was abrogated by the IL-1 inhibitor, the IL-1 receptor antagonist (IL-1Ra). On the contrary, non-IL-1alpha-expressing tumor cells ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0905509
更新日期:2006-07-01 00:00:00
abstract::The mannose receptor is a macrophage surface receptor that mediates both endocytosis and phagocytosis. Previous work has demonstrated that the prototypical Th 2 cytokine, interleukin-4 (IL-4), increases both cell-surface receptor expression and mannose receptor-mediated endocytosis, whereas the prototypical Th 1 cytok...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.64.1.108
更新日期:1998-07-01 00:00:00
abstract::Radiation-induced chimeric mice were used to study the origin of pulmonary alveolar macrophages. Unlike in other studies, these radiation chimeras were prepared by using a special fractionated irradiation regimen to minimize the killing of alveolar macrophage colony-forming cells, putative local stem cells. For this s...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:1987-11-01 00:00:00
abstract::The present study was initiated to identify the antigen recognized by the monoclonal antibody (MAb) MAC387 which is widely used for phenotypical characterization of myelomonocytic cells in situ. MAC387 has been described to show a similar reaction pattern as antisera to a complex formed by the calcium-binding proteins...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.55.2.259
更新日期:1994-02-01 00:00:00
abstract::Although endosomes, lysosomes, and phagosomes require a reductive environment for the optimal activity of disulfide reductases and other thiol-dependent enzymes, how these reductive environments are established and maintained remain unknown. Our goal in this study was to begin to elucidate the redox control systems re...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.2HI0315-083R
更新日期:2016-07-01 00:00:00
abstract::Neutrophils were classically described as powerful effectors of acute inflammation, and their main purpose was assumed to be restricted to pathogen killing through production of oxidants. As consequence, neutrophils also may lead to significant collateral damage to the healthy tissues, and after performing these tasks...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.1MA0420-634R
更新日期:2020-10-01 00:00:00
abstract::Chemotactic factors and phorbol esters may act synergistically to evoke neutrophil responses, but the mechanism of such interaction is not entirely clear. We investigated the combined effects of the chemotactic peptide n-formyl-methionyl-leucyl-phenylalanine (FMLP) and phorbol myristate acetate (PMA) on protein kinase...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.47.1.49
更新日期:1990-01-01 00:00:00
abstract::Tumor necrosis factor alpha (TNF-alpha) more than doubles tritiated thymidine ([3H]TdR) uptake in mouse macrophages stimulated by macrophage colony-stimulating factor (CSF-1). However, nothing is known of how TNF-alpha affects this increase or even whether it is manifested by increased cellular proliferation. Here we ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.54.1.65
更新日期:1993-07-01 00:00:00
abstract::MRL/fas(lpr) mice, which undergo a systemic autoimmune disease with similarities to systemic lupus erythematosus (SLE), display reduced pathology and prolonged survival if rendered deficient in ICAM-1. However, it remains unclear whether this is a result of the ability of ICAM-1 to promote the immune response or media...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1107796
更新日期:2008-07-01 00:00:00
abstract::The expression of beta 2-integrins on gammadelta T cells in naïve mice or those with experimental autoimmune encephalomyelitis (EAE) remains poorly characterized. We compared beta 2-integrin expression and cytokine production between gammadelta and alphabeta T cells over the acute course of EAE. We observed that unlik...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0407263
更新日期:2008-01-01 00:00:00
abstract::LPS has been widely used to induce inflammatory pain, attributing to production of inflammatory cytokines and sensitization of nociceptors. Paeoniflorin (PF) possesses anti-nociceptive property, but its effect on LPS-induced inflammatory pain has not been investigated. In this study, we aimed to investigate the analge...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.3MA0220-355R
更新日期:2020-07-01 00:00:00
abstract::Corynebacterium parvum-treated mice produce large amounts of circulating nitrogen oxides and develop a severe liver injury in response to lipopolysaccharide (LPS). Concurrent administration of NG-monomethyl-L-arginine not only suppresses nitric oxide synthesis in these animals but also profoundly increases the hepatic...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.52.4.390
更新日期:1992-10-01 00:00:00
abstract::In mammals, macrophages are multifunctional cells. Apart from their scavenger role in the clearance of non-self materials such as microorganisms and altered-self materials such as apoptotic cells, senescent erythrocytes, immune complexes, and inflammatory products, they play a crucial role in the regulation of both in...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1002/jlb.62.6.702
更新日期:1997-12-01 00:00:00
abstract::Cathelicidins comprise a family of mammalian proteins containing a C-terminal cationic antimicrobial domain that becomes active after being freed from the N-terminal cathelin portion of the holoprotein. Many other members of this family have been identified since the first cathelicidin sequences were reported 10 years...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.0403147
更新日期:2004-01-01 00:00:00
abstract::Interaction of bacterial lipopolysaccharide (LPS) with monocytes stimulates production of a variety of mediators that are involved in the pathogenesis of septic shock and wound repair. We report here the mechanisms of LPS uptake and intracellular distribution of LPS in human monocytes. Ficoll-Hypaque-purified peripher...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.48.4.316
更新日期:1990-10-01 00:00:00
abstract::Tissue macrophages produce several proteins of the complement system. The mechanisms that regulate this process are poorly understood. The established ability of certain prostaglandins to influence macrophage secretory activity suggests that these lipid mediators may also modulate complement production (CP). Using the...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.37.5.651
更新日期:1985-05-01 00:00:00