Abstract:
:Probabilities of disorder for FlgM proteins of 39 species whose optimal growth temperature ranges from 273 K (0°C) to 368 K (95°C) were predicted by a newly developed method called Sequence-based Prediction with Integrated NEural networks for Disorder (SPINE-D). We showed that the temperature-dependent behavior of FlgM proteins could be separated into two subgroups according to their sequence lengths. Only shorter sequences evolved to adapt to high temperatures (>318 K or 45°C). Their ability to adapt to high temperatures was achieved through a transition from a fully disordered state with little secondary structure to a semidisordered state with high predicted helical probability at the N-terminal region. The predicted results are consistent with available experimental data. An analysis of all orthologous protein families in 39 species suggests that such a transition from a fully disordered state to semidisordered and/or ordered states is one of the strategies employed by nature for adaptation to high temperatures.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Wang J,Yang Y,Cao Z,Li Z,Zhao H,Zhou Ydoi
10.1016/j.bpj.2013.10.026subject
Has Abstractpub_date
2013-12-03 00:00:00pages
2598-605issue
11eissn
0006-3495issn
1542-0086pii
S0006-3495(13)01193-4journal_volume
105pub_type
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