Abstract:
:This study describes the synthesis of a new generation of immunotoxins made by a noncovalent interaction between a monoclonal antibody derivatized with a dichlorotriazinic dye and the ribosomal inhibitor protein gelonin. The scheme of preparation has several advantages with respect to the traditional methods, which used heterobifunctional cross-linkers, such as a higher overall yield of production and the homogeneity of the obtained conjugate. Moreover, because no chemical derivatization of the gelonin was required, the unconjugated ribosome inactivating protein was recovered unaltered and therefore can be reused in other synthetic processes. This immunoconjugate was stable when tested in mouse serum and showed an interesting slow elimination rate when administered intravenously in mice. Although a high dye derivatization degree induced a modification of the specificity of the monoclonal antibody, the native specificity was restored after conjugation with gelonin. Furthermore the noncovalent linkage did not affect the gelonin inhibitory activity; in fact, the specific cytotoxic activity seemed to be similar to that of other disulfide-linked immunotoxins previously prepared in our laboratories.
journal_name
J Pharm Scijournal_title
Journal of pharmaceutical sciencesauthors
Dosio F,Brusa P,Delprino L,Grosa G,Ceruti M,Cattel Ldoi
10.1002/jps.2600830218subject
Has Abstractpub_date
1994-02-01 00:00:00pages
206-11issue
2eissn
0022-3549issn
1520-6017pii
S0022-3549(15)49342-6journal_volume
83pub_type
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