Conjugation of peptides to antisense interleukin-6 via click chemistry.

Abstract:

:Low molecular weight oligonucleotides have been discovered to have potential use for gene therapy by selectively inhibiting the expression of certain genes. Chemical conjugation of functional peptides to oligonucleotides can introduce desired properties to the oligonucleotides, such as cell-specific delivery, cellular uptake efficiency, and/or intracellular distribution. In this paper, targeting peptides are conjugated to antisense interleukin-6 via a copper (I) catalyzed alkyne-azide cycloaddition click reaction. A simple and reproducible solution-phase conjugation procedure was investigated. Oligonucleotide-peptide conjugations were characterized by reverse-phase high-pressure liquid chromatography and mass spectrometry. The results show that the targeting peptides can be used for targeting delivery of oligonucleotides using the aforementioned conjugation.

journal_name

Curr Med Chem

authors

Wang CF,Auriola S,Hirvonen J,Santos HA

doi

10.2174/0929867320666131119125045

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

1247-54

issue

10

eissn

0929-8673

issn

1875-533X

pii

CMC-EPUB-57517

journal_volume

21

pub_type

杂志文章
  • Stimuli-responsive Carriers for Controlled Intracellular Drug Release.

    abstract:BACKGROUND:Stimuli-responsive carriers are a class of drug delivery systems which can change their physicochemical properties and/or structural conformations in response to specific stimuli. Although passive and active drug targeting has proved to reduce the side effects to normal cells, controlled intracellular drug r...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867324666170830102409

    authors: Sheng Y,Hu J,Shi J,Lee LJ

    更新日期:2019-01-01 00:00:00

  • Attenuation of Diabetes-induced Cardiac and Subcellular Defects by Sulphur-containing Amino Acids.

    abstract:BACKGROUND:Patients with diabetes mellitus have an increased risk of mortality due to cardiovascular complications. Supplementation with specific sulphur-containing amino acids is rapidly emerging as a possible therapeutic adjuvant for diabetes and associated cardiovascular complications. OBSERVATIONS:It is well-known...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867324666170705115207

    authors: Tappia PS,Adameova A,Dhalla NS

    更新日期:2018-01-30 00:00:00

  • A Novel Class of Emerging Anticancer Compounds: Oxyprenylated Secondary Metabolites from Plants and Fungi.

    abstract::O-Prenyl secondary metabolites (3,3-dimethylallyl, geranyl-, farnesyl- and related biosynthetic derivatives) represent a class of rarely occurring natural products. In the last two decades such compounds have been found to exert promising and effective pharmacological activities, mainly in terms of anti-cancer propert...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867322666150716114758

    authors: Genovese S,Fiorito S,Epifano F,Taddeo VA

    更新日期:2015-01-01 00:00:00

  • Artificial Intelligence for epigenetics: towards personalized medicine.

    abstract::Epigenetics is a field of biological sciences focused on the study of reversible, heritable changes in gene function not due to modifications of the genomic sequence. These changes are the result of a complex cross-talk between several molecular mechanisms, that is in turn orchestrated by genetic and environmental fac...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章

    doi:10.2174/0929867327666201117142006

    authors: De Riso G,Cocozza S

    更新日期:2020-11-17 00:00:00

  • Nanomedicine: magnetic nanoparticles and their biomedical applications.

    abstract::During this past decade, science and engineering have seen a rapid increase in interest for nanoscale materials with dimensions less than 100 nm, which lie in the intermediate state between atoms and bulk (solid) materials. Their attributes are significantly altered relative to the corresponding bulk materials as they...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/092986710791959765

    authors: Banerjee R,Katsenovich Y,Lagos L,McIintosh M,Zhang X,Li CZ

    更新日期:2010-01-01 00:00:00

  • GCPII variants, paralogs and orthologs.

    abstract::Glutamate carboxypeptidase II (GCPII) and its splice variants, paralogs and human homologs represent a family of proteins with diverse tissue distribution, cellular localization and largely unknown function which have been explored only recently. While GCPII itself has been thoroughly studied from different perspectiv...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/092986712799462676

    authors: Hlouchová K,Navrátil V,Tykvart J,Sácha P,Konvalinka J

    更新日期:2012-01-01 00:00:00

  • Enhancement of chemotherapeutic efficacy by small molecule inhibition of NF-kappaB and checkpoint kinases.

    abstract::Apoptosis or programmed cell death is a cellular mechanism used to regulate cell number and eliminate damaged or mutated cells. Many chemotherapeutic agents and ionizing radiation induce not only apoptotic signaling pathways, but also survival responses such as DNA damage responses and cell cycle arrest, which allow f...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/092986707780362844

    authors: Sharma V,Hupp CD,Tepe JJ

    更新日期:2007-01-01 00:00:00

  • Brain excitatory/inhibitory circuits cross-talking with chromogranin A during hypertensive and hibernating states.

    abstract::To date, many scientific attempts have been directed towards the development of experimental models for the identification of neuronal mechanisms evoking cardiovascular and hemodynamic dysfunctions. The spontaneously hypertensive rat (SHR), a genetic model of essential hypertension, has become a valuable rodent for th...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/092986712802429993

    authors: Giusi G,Alò R,Avolio E,Zizza M,Facciolo RM,Talani G,Biggio G,Sanna E,Canonaco M

    更新日期:2012-01-01 00:00:00

  • Chemotherapy of breast cancer in the elderly.

    abstract::Breast cancer arises in about 48% of patients older than 65 years and more than 30% occurs in those over 70 years being the leading cause of cancer-related death in women older than 65. Elderly patients tolerate chemotherapy poorly compared to their younger counterpart because of progressive reduction of organ functio...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867053363261

    authors: Rossi A,Colantuoni G,Maione P,Ferrara C,Airoma G,Barzelloni ML,Castaldo V,Gridelli C

    更新日期:2005-01-01 00:00:00

  • Regulators of serine/threonine protein phosphatases at the dawn of a clinical era?

    abstract::Reversible phosphorylation is a key mechanism for regulating the biological activity of many human proteins that affect a diverse array of cellular processes, including protein-protein interactions, gene transcription, cell-cycle progression and apoptosis. Once viewed as simple house keeping enzymes, recent studies ha...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867023368836

    authors: Honkanen RE,Golden T

    更新日期:2002-11-01 00:00:00

  • New therapeutic strategies for coeliac disease: tissue transglutaminase as a target.

    abstract::Coeliac disease is a multifactorial disease characterized by a dysregulated immune response to ingested wheat gluten and related cereal proteins. With an incidence of about 1% of the general population, it is considered the most common food intolerance disorder. The mainstay of coeliac disease treatment is strict life...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/092986707782023343

    authors: Esposito C,Caputo I,Troncone R

    更新日期:2007-01-01 00:00:00

  • Neurotrophic factors--a tool for therapeutic strategies in neurological, neuropsychiatric and neuroimmunological diseases?

    abstract::Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) belong to the protein family of neurotrophins. They both display profound neuromodulatory functions and are essentially involved in the survival and homeostatic maintenance of central and peripheral neurons during development and adulthood. Moreove...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/092986707781745578

    authors: Schulte-Herbrüggen O,Braun A,Rochlitzer S,Jockers-Scherübl MC,Hellweg R

    更新日期:2007-01-01 00:00:00

  • The glioblastoma problem: targeting by combined medicinal chemistry approaches.

    abstract::Whilst knowledge of basic biology, diagnosis and prognosis of glioblastoma (GB - WHO grade IV) are steadily improving, advancements of therapy are discouragingly slow, with the only significant novelty during last ten years represented by introduction of temozolomide in chemotherapy. In order to analyze the current st...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867322666150530210700

    authors: Frosina G

    更新日期:2015-01-01 00:00:00

  • Use of click-chemistry in the development of peptidomimetic enzyme inhibitors.

    abstract::Cu(I)-catalyzed Azide-Alkyne Cycloaddition (CuAAC) is often utilized in medicinal chemistry to make the triazole moiety as it acts as a non-classical bioisostere of the peptide bond. This useful technique can also be applied in the fragment-based assembly of molecular libraries for high-throughput screening. This mini...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867321666131218093611

    authors: Fabbrizzi P,Menchi G,Guarna A,Trabocchi A

    更新日期:2014-01-01 00:00:00

  • Protein Interaction Domains and Post-Translational Modifications: Structural Features and Drug Discovery Applications.

    abstract:BACKGROUND:Many pathways regarding healthy cells and/or linked to diseases onset and progression depend on large assemblies including multi-protein complexes. Protein-protein interactions may occur through a vast array of modules known as Protein Interaction Domains (PIDs). OBJECTIVE:This review concerns with PIDs rec...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867326666190620101637

    authors: Vincenzi M,Mercurio FA,Leone M

    更新日期:2020-01-01 00:00:00

  • Structural approaches to explain the selectivity of COX-2 inhibitors: is there a common pharmacophore?

    abstract::The identification and characterisation of the isoenzyme cyclooxygenase 2 (COX-2) stimulated investigations to develop efficient non-steroidal anti-inflammatory drugs with reduced side effects compared to standard NSAIDs. This review will focus on the structural features needed to achieve COX-2 selectivity. Five struc...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867003374237

    authors: Dannhardt G,Laufer S

    更新日期:2000-11-01 00:00:00

  • Synthesis and biological activity of phosphonated nucleosides: part 1. Furanose, carbocyclic and heterocyclic analogues.

    abstract::Phosphonated nucleosides represent a promising alternative in the improvement of the biological activity of nucleoside analogues in antiviral and anticancer chemotherapy. The basic concept, the chemistry, the different structural modifications and their effects on the antiviral potency will be discussed in this review...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/092986706779026110

    authors: Piperno A,Chiacchio MA,Iannazzo D,Romeo R

    更新日期:2006-01-01 00:00:00

  • Arylpiperazine derivatives acting at 5-HT(1A) receptors.

    abstract::Serotonin (5-hydroxytryptamine, 5-HT) is one of the most attractive targets for medicinal chemists. Among 5-HTRs, the 5-HT(1A) subtype is the best studied and it is generally accepted that it is involved in psychiatric disorders such as anxiety and depression. Several structurally different compounds are known to bind...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867023371030

    authors: López-Rodríguez ML,Ayala D,Benhamú B,Morcillo MJ,Viso A

    更新日期:2002-02-01 00:00:00

  • Glutamate binding-site ligands of NMDA receptors.

    abstract::Excitatory neurotransmission mediated by NMDA (N-methyl-D-aspartic acid) receptors plays a key role in both healthy and diseased processes in the brain. Therefore, bioactive compounds that can interact selectively with these receptors have been the aim of extensive research in the search of effective therapeutic agent...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/092986711798347225

    authors: Bonaccorso C,Micale N,Ettari R,Grasso S,Zappalà M

    更新日期:2011-01-01 00:00:00

  • Progress and issues of the genome-wide association study for hypertension.

    abstract::Over the past few years, use of the genome-wide association study (GWAS) has made it possible to identify the primary genetic mechanisms of essential hypertension. GWAS results have helped identify many loci in or near genes that generally were not expected to be associated with blood pressure or essential hypertensio...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867321666141216124537

    authors: Pan S,Naruse H,Nakayama T

    更新日期:2015-01-01 00:00:00

  • Antipsychotic and antiepileptic drugs in bipolar disorder: the importance of therapeutic drug monitoring.

    abstract::Bipolar disorder (BD) is a long-term illness with mood swings which are characterized by recurrent episodes of mania/hypomania and depression, with variable interpolations of relatively asymptomatic periods, called euthymic, in which, however, some psychopathological symptoms may persist. Although mood stabilizers, su...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/092986709787909604

    authors: Musenga A,Saracino MA,Sani G,Raggi MA

    更新日期:2009-01-01 00:00:00

  • Development of DNA topoisomerase II-mediated anticancer agents, 3-(9-acridinylamino)-5-hydroxymethylanilines (AHMAs) and related compounds.

    abstract::A series of potential topoisomerase II-mediated anticancer 9-anilinoacridine derivatives, which are designed to avoid bio-oxidation and possessed long duration of drug action, is reviewed. Among these agents, 3-(9-acridinylamino)-5-hydroxymethylaniline (AHMA) derivatives and their alkylcarbamates have been investigate...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867023369231

    authors: Su TL

    更新日期:2002-09-01 00:00:00

  • Structure Properties and Mechanisms of Action of Naturally Originated Phenolic Acids and Their Derivatives against Human Viral Infections.

    abstract:BACKGROUND:A great effort has been made to develop efficacious antiviral drugs, but many viral infections are still lack of efficient antiviral therapies so far. The related exploration of natural products to fight viruses has been raised in recent years. Natural compounds with structural diversity and complexity offer...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867324666170815102917

    authors: Wu YH,Zhang BY,Qiu LP,Guan RF,Ye ZH,Yu XP

    更新日期:2017-01-01 00:00:00

  • Carbohydrate Scaffolds for the Study of the Autism-associated Bacterium, Clostridium bolteae.

    abstract::A large number of children in the autism spectrum disorder suffer from gastrointestinal (GI) conditions, such as constipation and diarrhea. Clostridium bolteae is a part of a set of pathogens being regularly detected in the stool samples of hosts affected by GI and autism symptoms. Accompanying studies have pointed ou...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867326666190225164527

    authors: Pequegnat B,Monteiro MA

    更新日期:2019-01-01 00:00:00

  • Non-antioxidant activities of vitamin E.

    abstract::Molecules in biological systems often can perform more than one function. In particular, many molecules have the ability to chemically scavenge free radicals and thus act in the test tube as antioxidant, but their main biological function is by acting as hormones, ligands for transcription factors, modulators of enzym...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867043365332

    authors: Zingg JM,Azzi A

    更新日期:2004-05-01 00:00:00

  • Protein-ligand docking in the new millennium--a retrospective of 10 years in the field.

    abstract::Protein-ligand docking is currently an important tool in drug discovery efforts and an active area of research that has been the subject of important developments over the last decade. These are well portrayed in the rising number of available protein-ligand docking software programs, increasing level of sophisticatio...

    journal_title:Current medicinal chemistry

    pub_type: 历史文章,杂志文章,评审

    doi:10.2174/0929867311320180002

    authors: Sousa SF,Ribeiro AJ,Coimbra JT,Neves RP,Martins SA,Moorthy NS,Fernandes PA,Ramos MJ

    更新日期:2013-01-01 00:00:00

  • MAP kinase p38 inhibitors: clinical results and an intimate look at their interactions with p38alpha protein.

    abstract::Mitogen-activated protein kinase p38 is a serine/threonine kinase originally isolated from lipopolysaccharide (LPS) stimulated monocytes. There are four isoforms p38alpha p38beta, p38gamma, and p38delta. The most thoroughly studied isoform is p38alpha, whose activation has been observed in many hematopoietic and non-h...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/092986705774462914

    authors: Lee MR,Dominguez C

    更新日期:2005-01-01 00:00:00

  • Recent Advances and Challenges in Steroid Metabolomics for Biomarker Discovery.

    abstract:BACKGROUND:Steroid hormones belong to a group of low-molecular weight compounds which are responsible for maintenance of various body functions, thus, their accurate assessment is crucial for evaluation of biosynthetic defects. The development of reliable methods allowing disease diagnosis is essential to improve early...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/0929867324666171113120810

    authors: Kotłowska A,Szefer P

    更新日期:2019-01-01 00:00:00

  • Evaluation of common unfavourable genetic variants in cerebrovascular diseases: recommendation for supportive genetic examinations and methodological approaches for common genetic variants.

    abstract::The present paper summarizes the possible roles of frequent and unfavourable genetic variants in cerebrovascular disorders, such as stroke and leukoaraiosis. It also approaches the topic theoretically from functional and mathematical points of view, which can help make the accumulating data on genetic variants more un...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:10.2174/092986709788803006

    authors: Szolnoki Z

    更新日期:2009-01-01 00:00:00

  • Glucagon-like peptide-1, a gastrointestinal hormone with a pharmaceutical potential.

    abstract::Glucagon-like peptide-1 (GLP-1) is an insulinotropic hormone secreted from endocrine cells in the gut mucosa in response to meal ingestion. It is an important incretin hormone; mice with a null mutation in the GLP-1 receptor gene develop glucose intolerance. In addition, it inhibits gastrointestinal secretion and moti...

    journal_title:Current medicinal chemistry

    pub_type: 杂志文章,评审

    doi:

    authors: Holst JJ

    更新日期:1999-11-01 00:00:00