Abstract:
:Isolated hepatocytes from rat liver in primary culture rapidly lost viability under hypoxic conditions. In the presence of glycine, L-alanine or L-serine loss of viability under hypoxic conditions was greatly retarded. Glycine and L-serine already showed significant protection from hypoxic injury at a concentration of 0.1 mM; at 10 mM, all three amino acids offered almost complete protection. Beside these standard amino acids, 1-aminocyclopropane-1-carboxylic acid (ACPC) and sarcosine significantly decreased hypoxic injury of the hepatocytes, although to a lesser extent. Other amino acids tested provided only slight protection or had no effect on hypoxic injury of the hepatocytes. In the presence of the protective amino acids neither the ATP content nor the lactate production of the hypoxic hepatocytes were significantly affected. The addition of glycine, L-alanine and L-serine led to marked membrane alterations (blebs). These alterations, however, occurred without loss of viability and were reversible upon reoxygenation after up to 4 h of hypoxia.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Brecht M,de Groot Hdoi
10.1007/BF00808120subject
Has Abstractpub_date
1994-02-01 00:00:00pages
25-35issue
1eissn
0939-4451issn
1438-2199journal_volume
6pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::Plasma levels and urinary amino acid excretions were estimated by high-performance liquid chromatography in 15 control subjects and 36 stone formers (SFs) classified according to the stone type: (1) 22 cases with calcium oxalate stones; (2) four cases with pure uric acid stones; (3) 10 cases with magnesium-ammonium ph...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-009-0359-z
更新日期:2010-05-01 00:00:00
abstract::Two novel cyclic hexapeptides, designated NW-G10 (1) and NW-G11 (2), were isolated from the fermentation broth of Streptomyces alboflavus 313. Their relative structures were elucidated on the basis of extensive spectroscopic analysis, and the absolute configurations of several constituent amino acids were determined b...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1303-1
更新日期:2012-11-01 00:00:00
abstract::The putative translation factor eIF5A is essential for cell viability and is highly conserved from archaebacteria to mammals. This factor is the only cellular protein that undergoes an essential posttranslational modification dependent on the polyamine spermidine, called hypusination. This review focuses on the functi...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-007-0533-0
更新日期:2007-08-01 00:00:00
abstract::The successful prediction of protein-folding rates based on the sequence-predicted secondary structure suggests that the folding rates might be predicted from sequence alone. To pursue this question, we directly predict the folding rates from amino acid sequences, which do not require any information on secondary or t...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1189-3
更新日期:2012-08-01 00:00:00
abstract::Glutaryl-CoA dehydrogenase deficiency (GDD), which is one of the most frequent organic acid disorders, is characterized by a specific age- and regional-dependent neuropathology. We hypothesized that the distinct brain damage in GDD could be caused by the main pathologic metabolites, the organic acids glutaric (GA) and...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s007260050003
更新日期:2000-01-01 00:00:00
abstract::Transglutaminases (TGs) are a large family of related and ubiquitous enzymes that catalyze the cross-linking of a glutaminyl residue of a protein/peptide substrate to a lysyl residue of a protein/peptide co-substrate. Considerable and intense progress has been made in the understanding of the chemistry, molecular biol...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-011-1011-2
更新日期:2012-02-01 00:00:00
abstract::Filamentous fungi of the genusHypocrea were grown on malt extract/peptone agar, the mycelia were extracted with dichloromethane/methanol, and the extracts were totally hydrolyzed with 6 N HCl (110°C, 24 h). The amino acids (AA) released from peptides were converted into theirN(O)-pen-tafluoropropionyl 1-propyl esters ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806923
更新日期:1991-06-01 00:00:00
abstract::The epidermal growth factor receptor (EGFR) represents an important molecular target for both radiotracer-based diagnostic imaging and radionuclide therapy of various cancer entities. For the delivery of radionuclides to the tumor, peptides hold great potential as a transport vehicle. With respect to EGFR, the peptide...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2616-5
更新日期:2018-10-01 00:00:00
abstract::For medical use of proteins and peptide-based drugs, it is desirable to have small biologically active sequences because they improve stability, reduce side effects, and production costs. Several plant defensins have their biological activities imparted by a sequence named γ-core. Vu-Def, a Vigna unguiculata defensin,...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02800-8
更新日期:2019-11-01 00:00:00
abstract::The purpose of this study was to investigate H(e) concentration in plasma from 80 healthy donors in relation to age (6 newborns are also included), sex, daily variation (9, 11 a.m.; 2, 6, 12 p.m.) and a period of 5 subsequent months. A significant correlation (r = 0.63, p < 0.001) was observed between plasma H(e) and ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806188
更新日期:1993-02-01 00:00:00
abstract::The objective of this study was to determine the dose as well as duration of exposure-dependent effects of L-alanyl-L-glutamine, arginine or taurine on polymorphonuclear neutrophil (PMN) free alpha-keto acid profiles and, in a parallel study, on PMN immune functions. Exogenous L-alanyl-L-glutamine significantly increa...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0223-8
更新日期:2005-11-01 00:00:00
abstract::In the article (Romek et al. 2013) we reported the values of δ15N (‰) and δ13C (‰) obtained by. ...
journal_title:Amino acids
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00726-017-2506-2
更新日期:2018-01-01 00:00:00
abstract::In the absence of exogenous Ca(2+) and Mg(2+) and in the presence of EGTA, which favours the release of endogenous Ca(2+), the polyamine spermine is able to stimulate the activity of pyruvate dehydrogenase complex (PDC) of energized rat liver mitochondria (RLM). This stimulation exhibits a gradual concentration-depend...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0099-5
更新日期:2009-03-01 00:00:00
abstract::A subline growing in medium without arginine and ornithine was established from a rat Reuber hepatoma cell line (R-Y121B·cho). The subline designated R-Y117B·cho was able to grow in glutamine, arginine and ornithine-free, glutamate-supplemented medium. Arginine synthesis from glutamate requires four urea cycle enzymes...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00807328
更新日期:1996-09-01 00:00:00
abstract::Synthesis and properties of a new fluorescent/fluorogenic substrate Ac-DEVD-AMAC for caspase-3 are reported. The substrate is obtained by conventional Fmoc-based solid phase peptide synthesis and its properties are investigated with regard to fluorescence, sensitivity, applicability and kinetic constants. A non-tradit...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0136-4
更新日期:2009-03-01 00:00:00
abstract::Spermine oxidase (SMO) was discovered much more recently than other enzymes involved in polyamine metabolism; this review summarizes 10 years of researches on this enzyme. Spermine oxidase (SMO) is a FAD-dependent enzyme that specifically oxidizes spermine (Spm) and plays a dominant role in the highly regulated mammal...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-011-1014-z
更新日期:2012-02-01 00:00:00
abstract::A variety of alpha-amino acids are enantioresolved for the first time on naphthylethylcarbamate-beta-cyclodextrin bonded phases (i.e., SN- and RN-beta-CD) using the acetonitrile-based mobile phase after their pre-column derivatization with phenyl isothiocyanate in alkaline medium. The resolution is better obtained on ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-003-0038-4
更新日期:2004-06-01 00:00:00
abstract::Seryl-histidine dipeptide (Ser-His) has been recognized as the shortest peptide with hydrolysis cleavage activity; however, its protein cleavage spectrum has not yet been fully explored. Here, four differently folded proteins were treated with Ser-His, and the digestion products were evaluated with high-resolution mas...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-017-2487-1
更新日期:2018-01-01 00:00:00
abstract::IEF is often used in multidimensional shotgun proteomics and the narrow range of 3.5-4.5 is the recommended pH interval for the fractionation of tryptic peptides. Usually, even if IEF is performed in IPG strip with a narrow range pH, the entire sample must be loaded onto the strip, including the "out of IPG range" pep...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1720-4
更新日期:2014-05-01 00:00:00
abstract::This study aimed to investigate the ability of curcumin to convert asparagine into acrylamide during heating at different temperatures. Binary and ternary model systems of asparagine-curcumin and asparagine-curcumin-fructose were used to determine the role of curcumin on acrylamide formation in competitive and uncompe...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1179-5
更新日期:2013-06-01 00:00:00
abstract::It is controversial whether homocysteic acid or other homocysteine derivatives show growth promoting effects. In a clonogenic assay we could show that homocysteine thiolactone and its alpha alkylated derivative increased colony formation significantly. Our work favorizes previous observations showing growth promoting ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00808453
更新日期:1994-02-01 00:00:00
abstract::This study investigated the effect of agmatine (Agm) in proliferation of ovine trophecdoderm cells (oTr1) as well as the importance of the arginine decarboxylase (ADC) and agmatinase (AGMAT) alternative pathway for synthesis of polyamines in ovine conceptuses during the peri-implantation period of pregnancy. Morpholin...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-017-2515-1
更新日期:2018-02-01 00:00:00
abstract::Granulocyte-colony stimulating factor stimulates production and antibacterial function of neutrophiles. Therapy using the recombinant protein drug represents a major step forward in oncology. The protein has not been, however, completely sequenced at the protein level and this formed the rationale of the current study...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0312-1
更新日期:2010-04-01 00:00:00
abstract::In previous studies we characterized arginine transporter genes from Trypanosoma cruzi and Leishmania donovani, the etiological agents of chagas disease and kala azar, respectively, both fatal diseases in humans. Unlike arginine transporters in higher eukaryotes that transport also lysine, these parasite transporters ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0812-z
更新日期:2012-01-01 00:00:00
abstract::Until now information about the influence of puberty on gingival tissue responses to Ni-Ti alloy haven't been available. Since our previous researches have demonstrated that Ni-Ti appliances have an influence on hyperplastic gingivopathy and data has pointed out a possible hormonal influence on the susceptibility of g...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s007260200001
更新日期:2002-01-01 00:00:00
abstract::Peptide bonds in protein structures are mainly found in trans conformation with a torsion angle ω close to 180°. Only a very low proportion is observed in cis conformation with ω angle around 0°. Cis-trans isomerization leads to local conformation changes which play an important role in many biological processes. In t...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-013-1511-3
更新日期:2013-08-01 00:00:00
abstract::The nucleotide (ATP-ADP)/nucleoside (adenosine) ratio in the circulation can modulate the processes of vasoconstriction, vasodilatation and platelet aggregation. The main objective of the present study with rat blood serum was to evaluate the possibility of changes in nucleotide hydrolysis by phenylalanine (Phe) and p...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-002-0345-1
更新日期:2003-06-01 00:00:00
abstract::The coupling between G protein-coupled receptors (GPCRs) and guanine nucleotide-binding proteins (G proteins) regulates various signal transductions from extracellular space into the cell. However, the coupling mechanism between GPCRs and G proteins is still unknown, and experimental determination of their coupling sp...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1133-6
更新日期:2012-08-01 00:00:00
abstract::Nitric oxide synthase (NOS) activities are responsible for the enzymatic conversion of L-arginine into NO and L-citrulline. Relatively low amounts of NO are produced in intestinal epithelial cells or are released from nerve endings. The effects of NO production are related to the maintenance of epithelial integrity an...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s007260050020
更新日期:2000-01-01 00:00:00
abstract::In an attempt to increase our knowledge regarding the mechanisms of surfactant membrane interaction, we studied the action of several anionic and cationic amino acid-based surfactants on membrane fluidity using fluorescence anisotropy. Anisotropy measurements demonstrated that almost all of the surfactants studied dis...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-006-0447-2
更新日期:2007-09-01 00:00:00