Abstract:
:Synthesis and properties of a new fluorescent/fluorogenic substrate Ac-DEVD-AMAC for caspase-3 are reported. The substrate is obtained by conventional Fmoc-based solid phase peptide synthesis and its properties are investigated with regard to fluorescence, sensitivity, applicability and kinetic constants. A non-traditional approach to assay the proteases activity using 2-aminoacridone labeled peptides is proposed. This approach utilizes the decrease of fluorescence intensity of a sample as a measure for the enzyme activity.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Lozanov V,Ivanov IP,Benkova B,Mitev Vdoi
10.1007/s00726-008-0136-4subject
Has Abstractpub_date
2009-03-01 00:00:00pages
581-6issue
3eissn
0939-4451issn
1438-2199journal_volume
36pub_type
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