Determination of free bile acids in pharmaceutical preparations by packed column supercritical fluid chromatography.


:A method was developed for the baseline separation of common free bile acids by supercritical fluid chromatography. A phenylbonded silica column, with UV detection at 210 nm, and carbon dioxide modified with methanol as the mobile phase were used. The influence of the stationary phase, modifier concentration, temperature, column pressure, and modifier identity on retention was studied. The separation obtained is at least eight times faster than those achieved for similar mixtures by the existing chromatographic techniques. This new procedure is applicable to the assay of ursodeoxycholic acid and chenodeoxycholic acid in capsule and tablet formulations. Individual dosage forms were disintegrated in methanol and an aliquot of the resulting suspension was filtered for the supercritical fluid chromatographic analysis. The method is rapid, accurate, and reproducible.


J Pharm Sci


Scalia S,Games DE




Has Abstract


1993-01-01 00:00:00














  • Automated in vitro dissolution rate analysis of potassium in plastic matrix slow release tablets.

    abstract::A fully automated system for dissolution rate analysis of potassium in slow release tablets is described. Aliquots are removed after 1, 2, and 4 hr from six samples, and potassium is analyzed in a flame photometer at 768 nm. A complete study of six samples takes 5.5 hr. The system may be run overnight. During the time...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Engdahl A,Karlberg B,Thelander S

    更新日期:1976-03-01 00:00:00

  • Studies on the structure of the complex of the boron neutron capture therapy drug, L-p-boronophenylalanine, with fructose and related carbohydrates: chemical and 13C NMR evidence for the beta-D-fructofuranose 2,3,6-(p-phenylalanylorthoboronate) structure.

    abstract::The complex of L-L-boronophenylalanine (L-p-BPA) with fructose has been used for the past 5 years in clinical trials of boron neutron capture therapy to treat both melanoma and glioblastoma multiforme. However, the structure of this complex in water buffered at physiologic pH has not been established. In the (1)H NMR ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Shull BK,Spielvogel DE,Head G,Gopalaswamy R,Sankar S,Devito K

    更新日期:2000-02-01 00:00:00

  • Effect of micellization on rate of cupric-ion-promoted hydrolysis of dicarboxylic acid hemiesters.

    abstract::The effect of micellar sodium lauryl sulfate on the rate of cupric-ion-promoted hydrolysis of some dicarboxylic acid hemiesters was investigated at pH 5, ionic strength 0.1 M, and 40, 45, and 50 degrees. The rate of cupric-ion-promoted hydrolysis of sodium n-decyloxalate in the micellar phase is about 50 times as fast...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Ong JT,Kostenbauder HB

    更新日期:1976-12-01 00:00:00

  • Neurogenic influences of bilateral adrenalectomy on monoamine oxidase.

    abstract::Bilateral adrenalectomy (10 days) increased the monoamine oxidase activity of the rat heart, vas deferens, spleen, superior cervical ganglion, and hypothalamus but not that of the rest of the brain, kidney, and liver. Experiments were made to determine whether the increased activity was due to neurogenic influences an...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Clarke DE,Sampath SS

    更新日期:1976-01-01 00:00:00

  • Potency of siRNA versus shRNA mediated knockdown in vivo.

    abstract::The intracellular delivery of small interfering RNA (siRNA) is a therapeutic strategy to transiently block gene expression. Two silencing RNA strategies utilize either synthetic double stranded RNA or plasmid DNA encoding a short hairpin RNA (shRNA). In the present study, we have quantitatively compared the potency of...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: McAnuff MA,Rettig GR,Rice KG

    更新日期:2007-11-01 00:00:00

  • Preparation and biological evaluation of tumor-specific Ara-C liposomal preparations containing RGDV motif.

    abstract::Arginine-glycine-aspartate (RGD) has been shown to be essential for the recognition of integrins overexpressed in tumor cells, especially during tumor invasion, angiogenesis, and metasis. In this study, a novel tetrapeptide, RGD-valine (RGDV), was designed and attached to the N position of 1-β-D-arabinofuranosylcytosi...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Wang F,Cui C,Ren Z,Wang L,Liu H,Cui G

    更新日期:2012-12-01 00:00:00

  • In vitro and in vivo evaluation of a sulfenamide prodrug of basic metformin.

    abstract::In the present study, a previously described sulfenamide prodrug of a basic antidiabetic drug, metformin, was evaluated further. This sulfenamide prodrug was designed to improve the permeability and consequently the oral absorption and bioavailability (F) of the highly water-soluble metformin. Bioactivation of the pro...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Huttunen KM,Leppänen J,Vepsäläinen J,Sirviö J,Laine K,Rautio J

    更新日期:2012-08-01 00:00:00

  • Tripeptide discriminations using circular dichroism detection.

    abstract::A general spectroscopic method is described that might be applied to validating amino acid sequences in peptides and protein fragments with a view to it becoming a routine procedure with which to characterize biotechnology drug products. The tripeptides are the L-enantiomers of GGA, GGH, GGI, GGL, GGF, GHG, LGG, and Y...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Purdie N,Province DW,Johnson EA

    更新日期:1999-07-01 00:00:00

  • Kinetics of drug action in disease states. XXXVI: Effect of cyclosporine on the pharmacodynamics and pharmacokinetics of a barbiturate (heptabarbital) in rats.

    abstract::Pretreatment with cyclosporine reportedly prolongs the effect of certain general anesthetics in humans and the sleeping time of mice after pentobarbital administration. This investigation was designed to determine the mechanism(s) of the cyclosporine-barbiturate interaction. Adult female Wistar rats received cyclospor...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Hoffman A,Levy G

    更新日期:1990-01-01 00:00:00

  • Effect of the enantiomers of flurbiprofen, ibuprofen, and ketoprofen on intestinal permeability.

    abstract::Numerous studies have demonstrated that the administration of nonsteroidal anti-inflammatory drugs (NSAIDs) increases small intestinal permeability, and this has been suggested to be a prerequisite to enteropathy. It is believed that the inhibitory effect of chiral NSAIDs on the synthesis of prostaglandins and hence t...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Davies NM,Wright MR,Russell AS,Jamali F

    更新日期:1996-11-01 00:00:00

  • Inhomogeneous Distribution of Components in Solid Protein Pharmaceuticals: Origins, Consequences, Analysis, and Resolutions.

    abstract::Successful development of stable solid protein formulations usually requires the addition of one or several excipients to achieve optimal stability. In these products, there is a potential risk of an inhomogeneous distribution of the various ingredients, specifically the ratio of protein and stabilizer may vary. Such ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章,评审


    authors: Nguyen KTT,Frijlink HW,Hinrichs WLJ

    更新日期:2020-01-01 00:00:00

  • The influence of hepatic transport on the distribution volumes and mean residence time of drug in the body and the accuracy of estimating these parameters by the traditional pharmacokinetic calculations.

    abstract::The influence of hepatic uptake and efflux, which includes passive diffusion and transporter-mediated component, on drug distribution volumes [steady-state volume of distribution (V(ss)) and terminal volume of distribution (V(β))], mean residence time (MRT), clearance, and terminal half-life is considered using a simp...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Berezhkovskiy LM

    更新日期:2011-11-01 00:00:00

  • Dosage intervals based on mean residence times.

    abstract::It is shown that a suitable dosage interval, tau, can be estimated by a factor times the sum of the mean residence time (MRT) of the central compartment (MRTC) and the MRT of the absorption site (MRTA). This value of tau will provide a ratio, maximum steady-state plasma concentration: minimum steady-state plasma conce...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Wagner JG

    更新日期:1987-01-01 00:00:00

  • Biochemical investigation of active intracellular transport of polymeric gene-delivery vectors.

    abstract::To design safe, efficient synthetic gene therapy vectors, it is desirable to understand the intracellular mechanisms that facilitate their delivery from the cell surface to the nucleus. Elements of the cytoskeleton and molecular motor proteins are known to play a pivotal role in most intracellular active transport pro...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Drake DM,Pack DW

    更新日期:2008-04-01 00:00:00

  • High-pressure liquid chromatographic determination of ibuprofen in plasma.

    abstract::A sensitive, simple, and rapid method for the quantitation of ibuprofen in plasma, using 1-(p-fluorobenzoyl)-5-methoxy-2-methylindole acetic acid as the internal standard, was developed. The method is based on reversed-phase high-pressure liquid chromatography with a mobile phase containing acetonitrile--0.1 M acetic ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Ali A,Kazmi S,Plakogiannis FM

    更新日期:1981-08-01 00:00:00

  • Frog intestinal sac: a new in vitro method for the assessment of intestinal permeability.

    abstract::The aim of this study was to evaluate a new experimental protocol utilizing isolated frog intestinal sacs for the assessment of intestinal drug permeability in humans. Segments of approximately 5.0 cm in length were used for these experiments. The intestinal sacs were filled with a solution of the appropriate drug in ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Trapani G,Franco M,Trapani A,Lopedota A,Latrofa A,Gallucci E,Micelli S,Liso G

    更新日期:2004-12-01 00:00:00

  • Simultaneous determination of nitrate and nitrite in toothpastes by high-performance liquid chromatography.

    abstract::A stability-indicating analytical method is described for the simultaneous determination of nitrate, and if present, its reductive degradation product, nitrite, in toothpastes. Nitrate and nitrite were extracted from the toothpaste using distilled water and separated from other water-soluble excipients by two RP-8 col...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Lulla H,Chen SS,Sena FJ

    更新日期:1984-07-01 00:00:00

  • Preformulation-Assisted Design and Characterization of Modified Release Gastroretentive Floating Extrudates Towards Improved Bioavailability and Minimized Side Effects of Baclofen.

    abstract::Baclofen immediate release mode of administration exhibit sharp plasma peaking that results in the emergence of side effects like hypotension. This research employs preformulation studies to design an optimum dosage form for baclofen to enhance therapeutic outcomes. These studies include partition coefficient and ex-v...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Ibrahim M,Naguib YW,Sarhan HA,Abdelkader H

    更新日期:2020-10-15 00:00:00

  • Trileucine improves aerosol performance and stability of spray-dried powders for inhalation.

    abstract::For particles to be useful medicinal aerosols, not only their aerodynamic diameter has to be on the order of a few micrometers but also they have to be chemically and physically stable. Manufacture of respirable particles is a technical challenge because as particles are reduced in size by conventional milling techniq...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Lechuga-Ballesteros D,Charan C,Stults CL,Stevenson CL,Miller DP,Vehring R,Tep V,Kuo MC

    更新日期:2008-01-01 00:00:00

  • Determination of diphenoxylate hydrochloride and atropine sulfate in solutions and tablets.

    abstract::Methods for the determination of diphenoxylate hydrochloride and atropine sulfate combinations in solutions and powdered tablet composites are presented. A semiautomated assay for diphenoxylate hydrochloride in individual tablets (content uniformity) also is presented. The USP XIX assays for these products are cumbers...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Ballbach RL,Brown DJ,Walters SM

    更新日期:1977-11-01 00:00:00

  • In Vitro Evaluation of Novel Phenytoin-Loaded Alkyd Nanoemulsions Designed for Application in Topical Wound Healing.

    abstract::Phenytoin-loaded alkyd nanoemulsions were prepared spontaneously using the phase inversion method from a mixture of novel biosourced alkyds and Tween 80 surfactant. Exposure of human adult keratinocytes (HaCaT cells) for 48 h to alkyd nanoemulsions producing phenytoin concentrations of 3.125-200 μg/mL resulted in rela...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Teo SY,Yew MY,Lee SY,Rathbone MJ,Gan SN,Coombes AGA

    更新日期:2017-01-01 00:00:00

  • Quantitative structure-pharmacokinetic/pharmacodynamic relationships of corticosteroids in man.

    abstract::The purpose of this study was to develop quantitative structure-activity/pharmacokinetic relationships (QSAR/QSPKR) for 11 selected corticosteroids in man. Multiple linear regression analysis with an automatic forward step-wise inclusion algorithm was used to construct QSAR/QSPKR models from molecular and submolecular...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Mager DE,Jusko WJ

    更新日期:2002-11-01 00:00:00

  • GLC-mass spectrometry of Teucrium polium oil.

    abstract::The essential oil of Teucrium polium, growing in Saudi Arabia, was thoroughly investigated for its constituents by GLC-mass spectrometry, TLC, and spectrophotometric methods. This investigation revealed the presence of 10 terpenoidal compounds including the hydrocarbons beta-pinene, limonene, alpha-phellandrene, and g...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Hassan MM,Muhtadi FJ,Al-Badr AA

    更新日期:1979-06-01 00:00:00

  • Prevention of molecular self-association by sodium salicylate: effect on methylene blue.

    abstract::Sodium salicylate improves the rectal absorption of drugs which exhibit molecular self-association; it is suggested that salicylate may improve drug bioavailability by altering the drug self-association pattern. Methylene blue was chosen as a model molecule for investigating the interference of salicylate with drugs u...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Touitou E,Fisher P

    更新日期:1986-04-01 00:00:00

  • Polynitro aromatic compounds in analytical chemistry II: Reaction of menadione with 2,4-dinitrophenylhydrazine.

    abstract::The intense blue color formed in the official assay of menadione injection by treatment of the sample with 2,4-dinitrophenylhydrazine and ammonia is shown by data from visible light spectra and mass spectra and by comparison of the pKa value with that of the corresponding reaction product of acetone to be due to proto...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Burns LB,Stedman RJ,Tuckerman MM

    更新日期:1978-02-01 00:00:00

  • Synthesis of 4-substituted aminoquinoline-3-carboxylates as potential antimicrobial agents.

    abstract::A series of 4-substituted aminoquinoline-3-carboxylates was prepared and evaluated for antimicrobial activity. Four of the compounds (VIII, XIII, XV, and XXIII) exhibited low activity against Staphylococcus aureus. ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Marecki PE,Bambury RE

    更新日期:1984-08-01 00:00:00

  • Pharmaceutical impurity identification: a case study using a multidisciplinary approach.

    abstract::A multidisciplinary team approach to identify pharmaceutical impurities is presented in this article. It includes a representative example of the methodology. The first step is to analyze the sample by LC-MS. If the structure of the unknown impurity cannot be conclusively determined by LC-MS, LC-NMR is employed. If th...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Alsante KM,Boutros P,Couturier MA,Friedmann RC,Harwood JW,Horan GJ,Jensen AJ,Liu Q,Lohr LL,Morris R,Raggon JW,Reid GL,Santafianos DP,Sharp TR,Tucker JL,Wilcox GE

    更新日期:2004-09-01 00:00:00

  • Osmotic water transport through cellulose acetate membranes produced from a latex system.

    abstract::The advisability of a progressive curtailment of organic solvent film coating offers an incentive to develop latex systems. Here, the use of aqueous colloidal dispersions of cellulose acetate, plasticized with water-soluble additives, is proposed as an alternative way to obtain cellulose acetate membranes either by ca...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Bindschaedler C,Gurny R,Doelker E

    更新日期:1987-06-01 00:00:00

  • X-ray diffraction and infrared spectroscopy of N,N-dimethylformamide and dimethyl sulfoxide solvatomorphs of betulonic acid.

    abstract::X-ray diffraction and infrared spectroscopy measurements for the N,N-dimethylformamide (DMF) and dimethyl sulfoxide (DMSO) solvatomorphs of betulonic acid (BA) were investigated. BA [3-oxolup-20(29)-en-28-oic acid, C(30)H(46)O(3)] exhibits a wide spectrum of biological activities and is considered to be a promising na...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Boryczka S,Jastrzebska M,Bębenek E,Kusz J,Zubko M,Kadela M,Michalik E

    更新日期:2012-12-01 00:00:00

  • Simultaneous GLC determination of methyl salicylate and menthol in a topical analgesic formulation.

    abstract::A simultaneous GLC determination for methyl salicylate and menthol in a topical analgesic formulation is reported. n-Nonadecane is used as the internal standard. The method is rapid, accurate, precise, and selective in the presence of salicylic acid and ethyl salicylate. ...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Sapio JP,Sethachutkul K,Moody JE

    更新日期:1979-04-01 00:00:00