Abstract：
:The purpose of the present study was to clarify the expression levels of transporter proteins in human lung tissue and to analyze regional and interindividual differences in primary cultured epithelial cells. Organic cation/carnitine tranporter 1 (OCTN1) protein expression was highest (2.08 ± 1.19 fmol/μg protein) in human lung tissue, followed by multidrug resistance-associated protein 1 (MRP1) and breast cancer resistance protein expression (1.41 ± 0.41, 1.30 ± 1.29 fmol/μg protein, respectively). Interestingly, the same expression levels of OATP2B1 protein were demonstrated among the epithelial cells derived from all pulmonary regions for the first time. These results suggest that OCTN1 may be the best target transporter protein for pulmonary disease drug design, and OATP2B1 may be an alternative target. MRP1 protein expression was also high and mainly localized in bronchial and alveolar regions. Regarding interindividual differences, the MRP1 protein showed a significant 18-fold maximal difference in the bronchial region among five donors. Sixteen of the 18 transporters showed higher expression in female lungs than in male lungs, especially MRP8 showed a 7.32-fold maximal difference. In conclusion, the protein expression profiles of pulmonary drug transporters and regional, gender, and interindividual differences were clarified. These findings may provide significant insights for pulmonary disease drug design and indicate that administration by inhalation may be viable.
journal_name
J Pharm Scijournal_title
Journal of pharmaceutical sciencesauthors
Sakamoto A,Matsumaru T,Yamamura N,Uchida Y,Tachikawa M,Ohtsuki S,Terasaki Tdoi
10.1002/jps.23606subject
Has Abstractpub_date
2013-09-01 00:00:00pages
3395-406issue
9eissn
0022-3549issn
1520-6017pii
S0022-3549(15)30951-5journal_volume
102pub_type
杂志文章abstract：:Photoprotective potential and biological consequences (mutagenic potential) of octyl-dimethyl-PABA (ODP), titanium dioxide (TiO2 ), and montmorillonite (MMT) upon ultraviolet B (UVB) irradiation, alone and in different associations [physical mixtures (PMs)], were evaluated using a Saccharomyces cerevisiae ogg1 mutant ...
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更新日期：2013-03-01 00:00:00
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更新日期：2012-01-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
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更新日期：2008-01-01 00:00:00
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更新日期：1998-06-01 00:00:00
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更新日期：2013-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/jps.2600840225
更新日期：1995-02-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.1002/jps.2600641127
更新日期：1975-11-01 00:00:00
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doi：10.1002/jps.2600740704
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pub_type： 杂志文章
doi：10.1016/j.xphs.2020.08.020
更新日期：2020-11-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.1002/jps.2600821218
更新日期：1993-12-01 00:00:00
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journal_title：Journal of pharmaceutical sciences
pub_type： 杂志文章
doi：10.1002/jps.2600820211
更新日期：1993-02-01 00:00:00
