Abstract:
:The objective of this study was to evaluate if the biologic membrane utilized for GTR can be impregnated by tetracycline hydrochloride and if the chemotherapeutic agent, once impregnated, can be released in minimal inhibitory concentrations for a period compatible with clinical application. Initially, an in vitro study was done with cellulose membranes cut in pieces measuring 9 cm2. A volume of 100 microliters containing a 72,000 micrograms/ml solution of tetracycline was dispensed onto each fragment, and dried for 70 minutes at 37 degrees C. Four pieces measuring 0.5 cm2 were cut from different points of the 9 cm2 membrane (presumably, containing 400 micrograms of tetracycline), placed in test tubes containing 4 ml of sterile deionized water, and agitated for 2 minutes. A standard curve was made from known concentrations of tetracycline and compared to 10 microliters of the test solutions obtained by the elution of the 0.5 cm2 fragments. The concentrations were determined through the bioassay technique in 3 duplicate experiments. The samples recovered from the membrane fragments had a mean of 101 micrograms/ml of tetracycline liberated, demonstrating that the membrane was impregnated homogeneously by the chemotherapeutic agent. In a second phase, an in vivo study was carried out to determine the length of time the drug was liberated from the membranes and at which concentrations, in the presence of an inflammatory process. Fourteen 0.5 cm2 fragments containing 400 micrograms of tetracycline were placed in 14 polypropylene chambers containing 200 microliters of thioglycolate medium. The chambers were implanted in the peritoneal cavities of 14 mice, one chamber per animal, and left in from 1 to 14 days. They were then removed and the concentrations of tetracycline determined from 20 microliters samples using a bioassay. The results showed that the antibiotic was released slowly from the 1st through the 12th day in decreasing concentrations that varied from 218 to 20.8 micrograms/ml. The impregnated cellulose membrane can probably be used in GTR acting as a membrane and as a slow-release device, liberating the chemotherapeutic agent in concentrations high enough to eliminate periodontopathic microorganisms.
journal_name
J Periodontoljournal_title
Journal of periodontologyauthors
Markman C,Fracalanzza SE,Novaes AB Jr,Novaes ABdoi
10.1902/jop.1995.66.11.978subject
Has Abstractpub_date
1995-11-01 00:00:00pages
978-83issue
11eissn
0022-3492issn
1943-3670journal_volume
66pub_type
杂志文章abstract:BACKGROUND:The minimum inhibitory concentration (MIC) does not provide information on the efficacy of antimicrobial agents against infections involving biofilms, which are many times more resistant than planktonic forms of bacteria. This report is on the design and initial trial of a device for growing standard biofilm...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2005.76.9.1443
更新日期:2005-09-01 00:00:00
abstract::The literature related to smoking and periodontal disease is reviewed. The effects of smoking on oral hygiene, gingivitis, necrotizing gingivitis, periodontitis, bacteria and the host's response are presented. From this review it is apparent that while the relationship between smoking and periodontal disease needs fur...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1986.57.10.617
更新日期:1986-10-01 00:00:00
abstract::A review of the 2006 and past American Dental Association (ADA) studies on the distribution of dentists provides information on the evolving number of periodontists. Despite an overall increase in the number of periodontists, major differences continue in practitioner/population ratios in different geographic areas. A...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.080592
更新日期:2009-05-01 00:00:00
abstract::The secretion of prostaglandin E2 (PGE2), tumor necrosis factor alpha (TNF alpha), interleukin 1 beta (IL-1 beta), and interleukin 6 (IL-6) by adherent mononuclear cells (AMNC) from 28 patients with early-onset periodontitis was studied. The early onset-periodontitis patients consisted of 12 patients with localized ju...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1994.65.2.139
更新日期:1994-02-01 00:00:00
abstract:BACKGROUND:The number of immunocompromised patients is increasing as a consequence of successful organ transplantation. Placing dental implants in these patients has been questioned because of their increased risk for infections. The 10-year follow-up data of a 71-year-old liver transplant recipient with long-term immu...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2004.75.6.909
更新日期:2004-06-01 00:00:00
abstract::Invasion of gingival tissues by bacteria is thought to be a major factor in development of periodontal lesions. Morphologic studies have revealed bacteria within the pocket epithelium, gingival connective tissues, alveolar bone, and oral epithelium. The current studies are intended to determine whether they are presen...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1990.61.1.35
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Valuable decision making for periodontal treatment success in situations where there is ambiguous or conflicting information was previously reported using a consensus building method, the Delphi survey, for posterior teeth with chronic periodontitis. This study focuses on outcome measures relevant in assessi...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2005.76.9.1508
更新日期:2005-09-01 00:00:00
abstract::Oral malodor was measured using a portable sulphide monitor in 2,672 individuals aged 18 to 64 years. In addition, dental (DMFT) and periodontal conditions (CPITN and attachment loss), dental plaque, and tongue coating status were assessed. Before clinical examination, subjects were interviewed about their oral health...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1995.66.8.679
更新日期:1995-08-01 00:00:00
abstract::Periodontal diseases are thought to result from inflammatory responses to bacterial challenges in the gingival crevicular area. Antibodies are a major host-protective mechanism in many bacterial infections. Consequently, the antibody responses to suspected periodontal pathogenic bacteria have been extensively measured...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1993.64.8s.807
更新日期:1993-08-01 00:00:00
abstract:BACKGROUND:A J-shaped relationship of body mass index (BMI) with severe periodontitis has been reported. However, it is unknown for other anthropometric indexes in young adults. METHODS:A cross-sectional study examined the relationships in 325 military men and women, aged 20 to 45 years in Taiwan. Anthropometric index...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.20-0275
更新日期:2020-11-03 00:00:00
abstract:BACKGROUND:Caspases are key mediators of apoptosis. Caspase-8 mediates extrinsic, and caspase-9 initiates the intrinsic pathway of apoptosis. Apoptosis Inducing Factor (AIF), a mitochondrial proapoptotic protein, mediates cell death by a caspase-independent process. Because apoptosis is involved in periodontal disease,...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.17-0716
更新日期:2019-03-01 00:00:00
abstract::Periodontal pockets of 11 subjects were irrigated once with either 1.64% SnF2 or saline applied by syringe. Changes on the total anaerobic colony forming units (CFU) and black pigmented bacteroides (BPBs) of the irrigated pockets were assessed for 31 days. The results showed little change of the CFUs by either single ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1985.56.6.330
更新日期:1985-06-01 00:00:00
abstract:BACKGROUND:The main problem in the posterior instrumental treatment of periodontitis is the re-epithelization of the periodontal defects, leading to the formation of an unphysiological, long junctional epithelium inhibiting the regeneration of periodontal attachment. A precondition for the re-epithelization is the inte...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1999.70.4.388
更新日期:1999-04-01 00:00:00
abstract:BACKGROUND:Chemically modified non-antimicrobial tetracyclines (CMTs) have been shown to inhibit pathologically elevated collagenase (and other matrix metalloproteinase, MMP) activity and bone resorption in vivo and in vitro. METHODS:In the current study, suboptimal doses of CMT-8 (a non-antimicrobial chemically modif...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2001.72.8.1069
更新日期:2001-08-01 00:00:00
abstract::Three wall intrabony defects were produced in 11 dogs using a round bur followed by curettes and hoes. A copper band was fixed to the tooth with stainless steel ligature wire. Six weeks later, the copper band was removed and the defect was treated with an enriched collagen solution (ECS) prepared from acid-extracted d...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1984.55.11.623
更新日期:1984-11-01 00:00:00
abstract:BACKGROUND:Prevalence of peri-implantitis is directly proportional to the time of functional loading. The aim of this retrospective study was to assess the survival and success rates as well as the incidence of peri-implantitis among patients with a history of periodontitis and among implants with 1 to 10 years of func...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.18-0715
更新日期:2019-12-20 00:00:00
abstract::A case of delayed eruption of the primary dentition secondary to phenytoin therapy in a 4-year-old child is reported. The patient had been on phenytoin therapy since the first months of life. Radiographs revealed that alveolar but not gingival emergence had occurred. Surgical excision of the excess gingival tissue was...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1984.55.1.19
更新日期:1984-01-01 00:00:00
abstract::The assessment of new attachment after periodontal treatment has been the focus of continuous research. An approach to longitudinally examine the deposition of cementum was devised by using fluorescence microscopy (FL), contact microradiography (CMR), and toluidine blue staining (TBS) after the injection of three labe...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1991.62.4.284
更新日期:1991-04-01 00:00:00
abstract::Several different types of interactions are possible between a chemical, a mixture of chemicals, and associated extrinsic factors (i.e., mechanical irritation) in the oral mucosa. These interactions can be broadly classified as irritative or allergenic in nature. In each case, the pathology usually includes mucosal in...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1998.69.6.620
更新日期:1998-06-01 00:00:00
abstract:BACKGROUND:To determine the level of perceived pain after different types of periodontal/oral surgical procedures, and the differences in patients' selection of pain management, over the counter (OTC) versus opioid, based on procedure type. METHODS:Patients undergoing surgical procedures were asked to complete a pain ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.20-0315
更新日期:2020-11-05 00:00:00
abstract::The plaque control record (O'Leary index) appears to be a commonly used oral hygiene index for assessing oral health skills. This index provides sufficient information for patient education; however, the time involved in data collection reduces its value. Most other indices limit the number of teeth and surfaces and f...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1998.69.10.1176
更新日期:1998-10-01 00:00:00
abstract:BACKGROUND:Periodontitis is a chronic inflammatory condition that leads to the destruction of the tooth-supporting tissues. Its treatment includes the arrest of the inflammatory process and, in some circumstances, the restoration of the lost anatomy and function, including the formation of new cementum, periodontal lig...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2010.090678
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:The analysis of samplings from periodontal pockets is important in the diagnosis and therapy of periodontitis. In this study, three different sampling techniques were compared to determine whether one method yielded samples suitable for the reproducible and simultaneous determination of bacterial load, cytok...
journal_title:Journal of periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1902/jop.2011.100565
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:Chronic periodontitis (CP) exhibits inflammation and alveolar bone loss, and severe forms of periodontitis are suggested to have a genetic basis. Vitamin D receptor (VDR) regulates bone metabolism and inflammation-related genes, and single nucleotide polymorphisms (SNPs) in the VDR gene may affect the functi...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.080465
更新日期:2009-04-01 00:00:00
abstract::Computer-based thermal imaging techniques were used to compare the rewarming rates of normal and inflamed human gingiva following cooling. A Modified Gingival Index (MGI) score, gingival crevicular fluid (GCF) measurement, and clinical photograph were obtained from a maxillary or mandibular anterior facial region in 2...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1989.60.11.628
更新日期:1989-11-01 00:00:00
abstract:BACKGROUND:One of the success factors in periodontal plastic surgery is the synergistic relationship between involved tissues and vascular supply. Gingiva as a functional unit is unique with a specific vascular configuration and contains the supracrestal portion naturally created to survive over avascular root surfaces...
journal_title:Journal of periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1902/jop.2012.110685
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:The aim of this study was to determine the plaque-removing ability of a curved bristle toothbrush compared to a conventional, straight bristle, manual toothbrush. METHODS:The study group consisted of 100 volunteers 16 to 24 years of age from a professional engineering college. A four-week post-prophylaxis, ...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1902/jop.2000.71.5.785
更新日期:2000-05-01 00:00:00
abstract:BACKGROUND:In the attempt to limit the post-surgery increase in buccal gingival recession (bREC), effect of a connective tissue graft (CTG) when combined with a buccal single flap approach (SFA) in the regenerative treatment of intraosseous defects is evaluated. METHODS:Data related to 30 patients with an intraosseous...
journal_title:Journal of periodontology
pub_type: 杂志文章,多中心研究
doi:10.1902/jop.2016.160471
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND:A 10-year follow-up study was conducted to clinically evaluate the long-term results after guided tissue regeneration (GTR) therapy of infrabony defects using non-resorbable and bioabsorbable barriers. METHODS:Twelve pairs of contralateral infrabony defects were treated in 12 subjects with advanced periodon...
journal_title:Journal of periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1902/jop.2008.070571
更新日期:2008-08-01 00:00:00
abstract::Clinical and laboratory data were compared in 72 patients with localized periodontitis (LP) and 103 patients with generalized periodontitis (GP). Significantly more LP than GP cases had decreased neutrophil chemotaxis (CTX), and were seropositive for Actinobacillus actinomycetemcomitans (Aa). Significantly, more GP ca...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1989.60.10.557
更新日期:1989-10-01 00:00:00