Abstract:
:The mitogen regulated protein/proliferin (MRP/PLF) gene is transcribed in primary mouse embryo fibroblasts (MEFs), but the pre-mRNA is not properly converted into a stable cytoplasmic mRNA and instead is rapidly degraded, apparently in the nucleus [Malyankar et al. (1994): Proc Natl Acad Sci USA 91:335-359]. In 3T3 cells derived from the MEFs by the standard 3T3 immortalization protocol, stable MRP/PLF mRNA is produced. We show here that the processing of intron sequences is similar in the two cell types and that some of the MRP/PLF transcripts are polyadenylated in the MEFs. We also document the production of stable MRP/PLF mRNA generated by transcription of various plasmid constructs containing different portions of the MRP/PLF3 gene after calcium phosphate-mediated transfection into the MEFs. We conclude that the inability of the MRP/PLF mRNA to accumulate in the MEFs is unlikely to result solely from a single localized sequence in the primary transcript (or the mRNA) that causes it to be subject to rapid breakdown; possibly export of the mRNA from the MEF nucleus is defective or some aspect of the transcriptional process marks the transcript for degradation.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Malyankar UM,Rittling SR,Denhardt DTdoi
10.1002/(sici)1097-4644(19960201)60:2<198::aid-jcbsubject
Has Abstractpub_date
1996-02-01 00:00:00pages
198-210issue
2eissn
0730-2312issn
1097-4644pii
10.1002/(SICI)1097-4644(19960201)60:2<198::AID-JCBjournal_volume
60pub_type
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