c-myc-enhanced S phase entry in keratinocytes is associated with positive and negative effects on cyclin-dependent kinases.

Abstract:

:The function of the c-myc proto-oncogene in cell cycle progression remains unclear. In order to examine the role c-myc may play in cell cycle progression, we have expressed the hormone-inducible MycER protein in the nontransformed, EGF-dependent mouse keratinocyte cell line BALB/MK. We have found that activation of MycER, but not a mutant MycER, Gal4ER, or FosER, leads to an EGF-dependent and hormone-dependent increased incorporation of labeled thymidine only during the S phase of the cell cycle in BALB/MK cells. A possible explanation for the increase in thymidine incorporation comes from flow cytometric analyses that reveal that activation of MycER leads to an increase in the total number of cells that enter S phase after EGF restimulation. Investigation of the intracellular effects of Myc activation shows that the expression of several putative Myc-sensitive proteins, cyclins A, E, and D1, and the E2F-1 protein are unaffected by Myc induction. Interestingly, we find that the histone H1 kinase activity associated with an E2F-1 complex containing Cyclin A and Cdk-2, but not that associated with Cyclin E, in late G1 and early S phases is increased in cells containing hormone-activated MycER, but not FosER. Although the mechanism for this Myc-dependent effect on E2F-1-associated kinase activity is still unknown, it does not appear to involve dissociation of the Cdk inhibitor p27Kip1 from the complexes as suggested by others. However, we have also found that hormone-treated cells actually show more p16INK4A inhibitor associated with another kinase, Cdk-4, as the cells are entering S phase. Altogether, the data suggest that the presence of excessive Myc protein in keratinocytes can stimulate otherwise noncycling cells to enter the cell cycle, and that this effect of Myc involves both positive effects on E2F-1-associated Cdk-2 and negative effects on Cdk-4 in late G1.

journal_name

J Cell Biochem

authors

Alexandrow MG,Moses HL

subject

Has Abstract

pub_date

1998-09-15 00:00:00

pages

528-42

issue

4

eissn

0730-2312

issn

1097-4644

pii

10.1002/(SICI)1097-4644(19980915)70:4<528::AID-JCB

journal_volume

70

pub_type

杂志文章
  • Investigation of nucleo-cytoplasmic transport using UV-guided microinjection.

    abstract::Active nucleo-cytoplasmic transport is mediated by dynamic signal-mediated pathways. We investigated the effects of transcription inhibitors or fluorescent lectins on nuclear import mediated by nuclear localization signals (NLSs). Therefore, a novel experimental approach that allows the controlled sequential introduct...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/1097-4644(20010301)80:3<388::aid-jcb130>3.

    authors: Stauber RH,Krätzer F,Schneider G,Hirschmann N,Hauber J,Rosorius O

    更新日期:2001-01-01 00:00:00

  • ERK 1/2 signaling pathway is involved in nicotine-mediated neuroprotection in spinal cord neurons.

    abstract::Evidence indicates that agonists of neuronal nicotinic receptors (nAChRs), including nicotine, can induce neuroprotective and anti-apoptotic effects in the CNS. To study these mechanisms, the present study focused on nicotine-mediated modulation of the extracellular regulated kinase 1 and 2 (ERK1/2) pathway in culture...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21013

    authors: Toborek M,Son KW,Pudelko A,King-Pospisil K,Wylegala E,Malecki A

    更新日期:2007-02-01 00:00:00

  • Endothelial cell fatty acid unsaturation mediates cold-induced oxidative stress.

    abstract::Ultraprofound hypothermia (< 5 degrees C) induces changes to cell membranes such as liquid-to-gel lipid transitions and oxidative stress that have a negative effect on membrane function and cell survival. We hypothesized that fatty acid substitution of endothelial cell lipids and alterations in their unsaturation woul...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20961

    authors: Zieger MA,Gupta MP,Siddiqui RA

    更新日期:2006-10-15 00:00:00

  • Identification of a domain within MDMX-S that is responsible for its high affinity interaction with p53 and high-level expression in mammalian cells.

    abstract::The MDMX gene product is related to the MDM2 oncoprotein, both of which interact with the p53 tumor suppressor. A novel transcript of the MDMX gene has been previously identified that has a short internal deletion of 68 base pairs, producing a shift in the reading frame after codon 114, resulting in the inclusion of 1...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10535

    authors: Rallapalli R,Strachan G,Tuan RS,Hall DJ

    更新日期:2003-06-01 00:00:00

  • Apolipoprotein E: a potent inhibitor of endothelial and tumor cell proliferation.

    abstract::Recombinant human apolipoprotein E3 (apoE), purified from E. coli, inhibited the proliferation of several cell types, including endothelial cells and tumor cells in a dose- and time-dependent manner. ApoE inhibited both de novo DNA synthesis and proliferation as assessed by an increase in cell number. Maximal inhibiti...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240540306

    authors: Vogel T,Guo NH,Guy R,Drezlich N,Krutzsch HC,Blake DA,Panet A,Roberts DD

    更新日期:1994-03-01 00:00:00

  • ATM and p53 combined analysis predicts survival in glioblastoma multiforme patients: A clinicopathologic study.

    abstract::Glioblastoma is one of the most malignant cancers, with a distinguishing dismal prognosis: surgery followed by chemo- and radiotherapy represents the current standard of care, and chemo- and radioresistance underlie disease recurrence and short overall survival of patients suffering from this malignancy. ATM is a kina...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26699

    authors: Romano FJ,Guadagno E,Solari D,Borrelli G,Pignatiello S,Cappabianca P,Del Basso De Caro M

    更新日期:2018-06-01 00:00:00

  • Kips off to Myc: implications for TGF beta signaling.

    abstract::Loss of sensitivity to the negative growth regulator transforming growth factor beta (TGF beta) is a feature of many different tumor types and is likely involved in tumor progression. In some cases this loss of sensitivity to TGF beta has been shown to be manifest in the absence of membrane-associated TGF beta recepto...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:

    authors: Alexandrow MG,Moses HL

    更新日期:1997-09-15 00:00:00

  • Effects of c-Jun and a negative dominant mutation of c-Jun on differentiation and gene expression in lens epithelial cells.

    abstract::We have used a retroviral vector (RCAS) to overexpress wild-type chicken c-Jun or a deletion mutant of chicken c-Jun (Jun delta 7) lacking the DNA binding region to investigate the possible role of c-Jun in lens epithelial cell proliferation and differentiation. Both constructs were efficiently expressed in primary cu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240580212

    authors: Rinaudo JA,Vacchiano E,Zelenka PS

    更新日期:1995-06-01 00:00:00

  • Suppression effect of seminal vesicle autoantigen on platelet-activating factor-induced mouse sperm capacitation.

    abstract::Mammalian sperm gain the ability to fertilize an egg successfully by the capacitation process. An unregulated capacitation process causes sperm to undergo a spontaneous acrosome reaction (AR) and resulting in loss of their fertilization activity. Thus, functional sperm activation is tightly regulated by a capacitation...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21050

    authors: Huang YH,Chen YH,Lin CM,Ciou YY,Kuo SP,Chen CT,Shih CM,Chang EE

    更新日期:2007-03-01 00:00:00

  • Characterization of the osteoblast-like cell phenotype under microgravity conditions in the NASA-approved Rotating Wall Vessel bioreactor (RWV).

    abstract::Weightlessness induces bone loss in humans and animal models. We employed the NASA-approved Rotating Wall Vessel bioreactor (RWV) to develop osteoblast-like cell cultures under microgravity and evaluate osteoblast phenotype and cell function. Rat osteoblast-like cell line (ROS.SMER#14) was grown in the RWV at a calcul...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Rucci N,Migliaccio S,Zani BM,Taranta A,Teti A

    更新日期:2002-01-01 00:00:00

  • Oral tumors in children: Diagnosis and management.

    abstract::Oral tumors are one of important tumors which could be associated with serious problems in infant and children. It has been showed that a variety of cellular and molecular pathways including genetics and epigenetics mechanisms (eg, chromosomal alterations, and microRNA) involved in pathogenesis events present in oral ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.26316

    authors: Khanmohammadi R,Mir F,Baniebrahimi G,Mirzaei H

    更新日期:2018-03-01 00:00:00

  • Molecular interaction of investigational ligands with human brain acetylcholinesterase.

    abstract::Alzheimer's disease, a neurodegenerative disorder continues to be an area of investigation by the international researchers' fraternity. Despite all the ongoing efforts, the effective set of promising cholinesterase inhibitors available in the market for patients' use is limited. Furthermore, the currently available d...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28461

    authors: Shakil S

    更新日期:2019-02-11 00:00:00

  • Emerging views of integrin signaling: implications for prostate cancer.

    abstract::Integrins are heterodimeric transmembrane cellular receptors that link the cell to its underlying substratum. Alterations in integrin expression and signaling have been implicated in many aspects of tumorigenesis and metastasis including cell survival, migration, and invasion. In prostate cancer, the progression from ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.10665

    authors: Slack-Davis JK,Parsons JT

    更新日期:2004-01-01 00:00:00

  • The association of the placental CASPASE-3 gene polymorphisms and preeclampsia susceptibility and in-silico analysis.

    abstract::Preeclampsia is a pathologic complication of pregnancy, associated with increased apoptosis in the cytotrophoblasts as the main cause of this disorder. Caspase-3 is a key apoptosis-related enzyme that both mitochondrial and death receptor apoptotic pathways can activate. In this study, we aimed to investigate the effe...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26869

    authors: Teimoori B,Yazdi A,Rezaei M,Mohammadpour-Gharehbagh A,Jahantigh D,Salimi S

    更新日期:2018-08-01 00:00:00

  • Insights into the mode of flavin mononucleotide binding and catalytic mechanism of bacterial chromate reductases: A molecular dynamics simulation study.

    abstract::Enzymes from natural sources protect the environment via complex biological mechanisms, which aid in reductive immobilization of toxic metals including chromium. Nevertheless, progress was being made in elucidating high-resolution crystal structures of reductases and their binding with flavin mononucleotide (FMN) to u...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28960

    authors: Pradhan SK,Singh NR,Dehury B,Panda D,Modi MK,Thatoi H

    更新日期:2019-10-01 00:00:00

  • Induction of hepatocyte growth factor expression by maleic acid in human fibroblasts through MAPK activation.

    abstract::Carboxylic acids have various biological activities and play critical roles in cellular metabolic pathways such as the tricarboxylic acid (TCA) cycle. It has been shown that some carboxylic acids induce cell proliferation and production of cytokines or growth factors. However, there have been no reports on effects of ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21724

    authors: Motoki T,Sugiura Y,Matsumoto Y,Tsuji T,Kubota S,Takigawa M,Gohda E

    更新日期:2008-07-01 00:00:00

  • Curcumin prevents lipopolysaccharide-induced atrogin-1/MAFbx upregulation and muscle mass loss.

    abstract::Because elevated ubiquitin ligase atrogin-1/MAFbx and MuRF1 mediate skeletal muscle wasting associated with various catabolic conditions, the signaling pathways involved in the upregulation of these genes under pathological conditions are considered therapeutic targets. AKT and NF-kappaB have been previously shown to ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21060

    authors: Jin B,Li YP

    更新日期:2007-03-01 00:00:00

  • Osteosarcoma cells enhance angiogenesis visualized by color-coded imaging in the in vivo Gelfoam® assay.

    abstract::We previously described a color-coded imaging model that can quantify the length of nascent blood vessels using Gelfoam® implanted in nestin-driven green fluorescent protein (ND-GFP) nude mice. In ND-GFP mice, nascent blood vessels are labeled with GFP. We report here that osteosarcoma cells promote angiogenesis in th...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24799

    authors: Uehara F,Tome Y,Miwa S,Hiroshima Y,Yano S,Yamamoto M,Mii S,Maehara H,Bouvet M,Kanaya F,Hoffman RM

    更新日期:2014-09-01 00:00:00

  • Control of expression and maturation of epidermal growth factor receptor.

    abstract::Epidermal growth factor (EGF)-receptor is a 170 kDa transmembrane glycoprotein that contains a ligand-binding external domain, and a catalytically active cytoplasmic region. The expression of this receptor as well as a 100 kDa homolog that contains only the external domain is enhanced by the ligand EGF. EGF acts at tr...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240440106

    authors: Das M,Kesavan P,Khire J

    更新日期:1990-09-01 00:00:00

  • Let's think again about using mammalian temperature-sensitive mutants to investigate functional molecules-The perspectives from the studies on three mutants showing chromosome instability.

    abstract::This review evaluates the use of temperature-sensitive (ts) mutants to investigate functional molecules in mammalian cells. A series of studies were performed in which mammalian cells expressing functional molecules were isolated from ts mutants using complementation by the introduction and expression of the responsib...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.27205

    authors: Sugaya K

    更新日期:2018-09-01 00:00:00

  • The presence of extracellular matrix alters the chondrocyte response to endoplasmic reticulum stress.

    abstract::The objective of this study was to test the hypothesis that extracellular matrix (ECM) would alter the endoplasmic reticulum (ER) stress response of chondrocytes. Chondrocytes were isolated from calf knees and maintained in monolayer culture or suspended in collagen I to form spot cultures (SCs). Our laboratory has sh...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.23025

    authors: Nugent AE,McBurney DL,Horton WE Jr

    更新日期:2011-04-01 00:00:00

  • Activation of cellular phospholipase A2 by Clostridium difficile toxin B.

    abstract::C. difficile toxin B is a potent cytotoxin known to disrupt the microfilaments of cultured cells. We have recently shown also increased phospholipase A2 activity in cells treated with toxin B. The activity was detected as a toxin-induced, dose-dependent release of 14C-arachidonic acid from prelabeled fibroblasts. Here...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240520115

    authors: Shoshan MC,Florin I,Thelestam M

    更新日期:1993-05-01 00:00:00

  • Modifications of nuclear architecture and chromatin organization in ataxia telangiectasia cells are coupled to changes of gene transcription.

    abstract::Ataxia telangiectasia (AT) is a rare genetic disorder caused by mutations of ATM gene. ATM kinase is a "master controller" of DNA-damage response and signal transducer of external stimuli. The complex role of ATM may explain the pleiotropic phenotype characteristic of AT syndrome, only partially. In our hypothesis, th...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20895

    authors: Grattarola M,Borghi C,Emionite L,Lulli P,Chessa L,Vergani L

    更新日期:2006-11-01 00:00:00

  • TLR3 signaling in a hepatoma cell line is skewed towards apoptosis.

    abstract::Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns (PAMPS) leading to the activation of the innate immune response and subsequently to the shaping of the adaptive immune response. Of the known human TLRs, TLR3, 7, 8, and 9 were shown to recognize nucleic acid ligands. TLR3 signaling is induced...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21119

    authors: Khvalevsky E,Rivkin L,Rachmilewitz J,Galun E,Giladi H

    更新日期:2007-04-01 00:00:00

  • Constitutive and inducible expression of cytochromes P4501A (CYP1A1 and CYP1A2) in normal prostate and prostate cancer cells.

    abstract::Constitutive and benzo[a]pyrene (B[a]P) inducible expression of CYP1A1 and CYP1A2 in prostate cancer and normal prostate epithelial cells were examined by immunoblotting. Androgen independent prostate cancer cell lines DU145 and PC3 have constitutive expression of CYP1A and CYP1A1 and CYP1A2, respectively. Four microm...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10753

    authors: Sterling KM Jr,Cutroneo KR

    更新日期:2004-02-01 00:00:00

  • Nongenomic effects of an anti-idiotypic antibody as an estrogen mimetic in female human and rat osteoblasts.

    abstract::We investigated the early effects of the anti-idiotypic antibody (clone 1D5), which recognized the estrogen receptor (ER), on cytosolic free calcium concentration ([Ca2+]i) and its long term effects on creatine kinase (CK) specific activity in female human and rat osteoblasts. These actions were compared to the known ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4644(199704)65:1<53::aid-jcb6>3

    authors: Sömjen D,Kohen F,Lieberherr M

    更新日期:1997-04-01 00:00:00

  • Molecular cloning of a tumor promoter-inducible mRNA found in JB6 mouse epidermal cells: induction is stable at high, but not at low, cell densities.

    abstract::From the mouse JB6 epidermal cell line C122 we have isolated a cDNA clone representing a 1.6-kilobase mRNA, called 2ar, that exhibits a biphasic induction in response to 12-O-tetradecanoyl-phorbol-13-acetate (TPA). The first phase of induction in subconfluent cells is transient, peaking at 6 h after the addition of TP...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240340103

    authors: Smith JH,Denhardt DT

    更新日期:1987-05-01 00:00:00

  • In Vitro Antitumor Effects of AHR Ligands Aminoflavone (AFP 464) and Benzothiazole (5F 203) in Human Renal Carcinoma Cells.

    abstract::We investigated activity and mechanism of action of two AhR ligand antitumor agents, AFP 464 and 5F 203 on human renal cancer cells, specifically examining their effects on cell cycle progression, apoptosis, and migration. TK-10, SN12C, Caki-1, and ACHN human renal cancer cell lines were treated with AFP 464 and 5F 20...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26114

    authors: Luzzani GA,Callero MA,Kuruppu AI,Trapani V,Flumian C,Todaro L,Bradshaw TD,Loaiza Perez AI

    更新日期:2017-12-01 00:00:00

  • Effects of serum on calcium mobilization in the submandibular cell line A253.

    abstract::The effects of serum on inositol 1,4,5-trisphosphate (IP3) formation and Ca2+ mobilization in the human submandibular cell line A253 were studied. Exposure of A253 cells to fetal bovine serum (FBS) elicited a 3.3-fold increase in IP3 formation and a concentration-dependent transient increase in cytosolic free Ca2+ con...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Sun X,Mörk AC,Helmke RJ,Martinez JR,Zhang GH

    更新日期:1999-06-15 00:00:00

  • The SIE, SRE, CRE, and FAP-1 four intracellular signal pathways between stimulus and the expression of c-fos promoter.

    abstract::c-fos gene has a close relationship with the osteoblasts. Mechanical signal effect on osteoblasts would change the expression level of c-fos. Authors introduce the signal pathways of four cis-response elements on the promoter of c-fos, that is, CRE (cAMP responsive element), FAP-1 (Fbs-AP-1 site), SRE (serum response ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22058

    authors: He H,Ping F

    更新日期:2009-04-01 00:00:00