Abstract：
:p150(glued) is the largest subunit of dynactin protein complex, through which cargo vesicles link to the microtubule minus-end directed motor protein dynein. In addition, p150(glued) also locates in the mother centriole where it organizes the subdistal appendage. The components of appendage are dynamically regulated throughout the cell cycle stages, but it is still unclear whether the centrosomal residency of p150(glued) correlated with cell cycle progression. Here we found that p150(glued) was located in the mother centriole during G1/S stage and its centrosomal residency was independent of microtubule transportation. However, the centrosomal p150(glued) became blurred at G2/M phase and this event was not regulated by its phosphorylation. Entering into mitosis, p150(glued) was robustly enriched in the mitotic spindle nearby the spindle poles but not in the centrosome. During serum starvation (G0 stage), p150(glued) appeared at the base of primary cilium and its depletion attenuated starvation-induced primary cilium formation. We also checked its role in the maintenance of centrosome homeostasis and configuration, and found depletion of p150(glued) did not induce centrosome amplification or splitting but inhibited U2OS cell growth. G1 arrest and reduced EdU incorporation were observed in p150(glued) deficient U2OS cells. In addition, cyclin E was downregulated following p150(glued) depletion. The p53/p21 signaling was activated indicating that CDKs were inactivated. The reduced cell growth was ameliorated in the p150(glued) depleted cells when treated with p53 inhibitor. Thus, we have identified the centrosomal targeting of p150(glued) in distinct cell cycle stage and uncovered its role in controlling G1/S transition.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Chen TY,Syu JS,Han TY,Cheng HL,Lu FI,Wang CYdoi
10.1002/jcb.25160subject
Has Abstractpub_date
2015-09-01 00:00:00pages
2049-60issue
9eissn
0730-2312issn
1097-4644journal_volume
116pub_type
杂志文章abstract：OBJECTIVE:To investigate the correlation between serum protein level of insulin growth factor 1 (IGF-1) and the degree of liver fibrosis in patients with chronic hepatitis C (CHC) combined with type 2 diabetes mellitus (T2DM). METHODS:The cases are divided into four groups. Then serum levels of IFG-1, alanine aminotra...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.27267
更新日期：2018-11-01 00:00:00
abstract：:Previous work has suggested that changes in nuclear membrane cholesterol may induce a stimulation in nuclear nucleoside triphosphatase (NTPase) activity. The purpose of the present study was to directly investigate if nuclear membrane cholesterol can stimulate nuclear NTPase activity. The cholesterol content of nuclei...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(sici)1097-4644(19961215)63:4<442::aid-jcb
更新日期：1996-12-15 00:00:00
abstract：OBJECTIVE:To clarify the mechanism of heat shock protein 27 (HSP27) as a diagnostic biomarker in coronary heart disease (CHD) and atherosclerosis (AS). METHOD:Expressions of HSP27 in patients with CHD and healthy controls were determined by enzyme-linked immunosorbent assay and the expressions of HSP27 in aortas of pa...
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更新日期：2019-03-01 00:00:00
abstract：:Epidermal growth factor (EGF)-receptor is a 170 kDa transmembrane glycoprotein that contains a ligand-binding external domain, and a catalytically active cytoplasmic region. The expression of this receptor as well as a 100 kDa homolog that contains only the external domain is enhanced by the ligand EGF. EGF acts at tr...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240440106
更新日期：1990-09-01 00:00:00
abstract：:The early 1980s revelation of cis-acting genomic elements, known as transcriptional enhancers, is still regarded as one of the fundamental discoveries in the genomic field. However, only with the emergence of genome-wide techniques has the genuine biological scope of enhancers begun to be fully uncovered. Massive scie...
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doi：10.1002/jcb.24854
更新日期：2014-11-01 00:00:00
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journal_title：Journal of cellular biochemistry
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doi：10.1002/jcb.20535
更新日期：2005-10-15 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.240450107
更新日期：1991-01-01 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(sici)1097-4644(19991215)75:4<566::aid-jcb
更新日期：1999-12-15 00:00:00
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pub_type： 杂志文章
doi：10.1002/jcb.21060
更新日期：2007-03-01 00:00:00
abstract：:The role of circular RNAs (circRNAs) in regulating cartilage homeostasis in osteoarthritis (OA) has been reported. However, the regulatory mechanisms of circRNAs in OA synovium remains basically unidentified. The current study intended to divulge the expression profile of circRNAs in OA synovium and investigate the po...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.29106
更新日期：2019-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/jcb.28106
更新日期：2018-11-26 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1002/jcb.26663
更新日期：2018-07-01 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22035
更新日期：2009-02-15 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22936
更新日期：2011-02-01 00:00:00
abstract：:The sequences from several independent cDNA clones encoding the chicken vitamin D receptor as well as primer extension assay have clearly delineated the 5' terminus and the transcriptional start site. Screening a chicken genomic library produced genomic clones containing vitamin D receptor (VDR) gene fragments. Restri...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:Hepatocyte growth factor (HGF) is known to have a number of biological properties including promoting tumor progression of human carcinomas. Metastasis involves a number of events that are attributed to induction by paracrine factors such as HGF. Identification of natural inhibitors of these events would allow better ...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4644(19960915)62:4%3C443::AID-J
更新日期：1996-09-15 00:00:00
abstract：:SCN- binds to the charged amino group of lysines, inducing local changes in the electrostatic free energy of histones. We exploited this property to selectively perturb the histone-DNA interactions involved in the stabilization of eu and heterochromatin. Differential scanning calorimetry (DSC) was used as leading tech...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.20689
更新日期：2006-03-01 00:00:00
abstract：:The effects of a ligand regulated neu tyrosine kinase were examined in NIH 3T3 cells. A chimeric construct encoding the human EGF receptor extracellular domain fused to the tyrosine kinase domain of the rat neu cDNA was expressed under the transcriptional control of the Moloney murine leukemia virus LTR promoter. This...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240420303
更新日期：1990-03-01 00:00:00
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journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.240290103
更新日期：1985-01-01 00:00:00
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doi：10.1002/jcb.20993
更新日期：2006-12-15 00:00:00
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更新日期：2006-10-15 00:00:00
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doi：10.1002/jcb.25178
更新日期：2015-10-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1002/jcb.10413
更新日期：2003-03-01 00:00:00
abstract：:MicroRNAs (miRNAs) have been identified as key players in cardiogenesis and heart pathophysiological processes. However, many miRNAs are still not recognized for their roles in cardiomyocytes differentiation. In this study, we evaluated the effects of microRNA-218 (miR-218) in cardiomyocyte differentiation of the mous...
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doi：10.1002/jcb.27721
更新日期：2019-03-01 00:00:00
abstract：:Lysozyme is expressed in serous, but not mucous, cells of the tracheobronchial glands and thereby constitutes a marker of the serous cell lineage in these glands. To identify DNA regulatory elements and transcription factors mediating the commitment of progenitor cells to the serous cell lineage, we have characterized...
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doi：10.1002/(SICI)1097-4644(19960601)61:3%3C350::AID-J
更新日期：1996-06-01 00:00:00
abstract：:We provided evidence that calcium-calmodulin plays a major role in bradykinin-induced arachidonic acid release by bovine aortic endothelial cells. In cells labeled for 16 hr with 3H-arachidonic acid, ionomycin and Ca2(+)-mobilizing hormones such as bradykinin, thrombin and platelet activating factor induced arachidoni...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4644(19961201)63:3%3C292::AID-J
更新日期：1996-12-01 00:00:00
abstract：:We observed that treatment of prostate cancer cells for 24 h with magnolol, a phenolic component extracted from the root and stem bark of the oriental herb Magnolia officinalis, induced apoptotic cell death in a dose- and time-dependent manner. A sustained inhibition of the major survival signal, Akt, occurred in magn...
journal_title：Journal of cellular biochemistry
pub_type： 杂志文章
doi：10.1002/jcb.22098
更新日期：2009-04-15 00:00:00
abstract：:Breast cancer frequently metastasizes to the bone, often leading to the formation of osteolytic lesions. This work compares the paracrine-induced osteoclastogenesis mediated by four human breast cancer cell lines, the estrogen-receptor positive T47D and MCF-7 and the estrogen-negative SK-BR-3 and Hs-578T cell lines. H...
journal_title：Journal of cellular biochemistry
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更新日期：2012-03-01 00:00:00
abstract：:Follicle-stimulating hormone-follicle-stimulating hormone receptor (FSH-FSHR) interaction is one of the most thoroughly studied signaling pathways primarily because of being implicated in sexual reproduction in mammals by way of maintaining gonadal function and sexual fertility. Despite material advances in understand...
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pub_type： 杂志文章
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更新日期：2018-11-02 00:00:00
abstract：:Thymoquinone (TQ) is the major active compound derived from the medicinal Nigella sativa. A few studies have shown that TQ exhibits anti-inflammatory activities in experimental models of rheumatoid arthritis (RA) through mechanisms that are not fully understood. The aim of this work was to evaluate the in vitro and in...
journal_title：Journal of cellular biochemistry
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doi：10.1002/jcb.22884
更新日期：2011-01-01 00:00:00
