Abstract：
:The xynA gene encoding a major xylanase of Clostridium stercorarium F-9 was sequenced. The structural gene consists of an open reading frame of 1533 bp encoding a protein of 511 amino acids with an M(r) of 56,519. XynA consists of a catalytic domain belonging to family G at the NH2-terminus and two direct repeats of about 90 amino acids with a short spacing at the COOH-terminus. The repeated sequences, CBDI and CBDII, were not homologous with amino acid sequences of the CBDs classified into families I to V. Nevertheless, XynA showed an affinity for insoluble cellulose such as Avicel. Binding of XynA to Avicel was strongly dependent on the concentration of the incubation buffer and was inhibited by Triton X-100. XynA bound to Avicel (2.4 nmol/g-cellulose) and acid-swollen cellulose (180 nmol/g-cellulose), suggesting that this enzyme has higher affinity for amorphous cellulose than for crystalline cellulose. Functions of CBDI and CBDII were investigated by constructing the mutant enzymes and evaluating the cellulose-binding ability of each of them. XynA4 lacking CBDI and XynA5 lacking CBDII bound to Avicel to a lesser extent than the parental enzyme XynA; but XynA6, devoid of both CBDs, did not bind at all, indicating that CBDI and CBDII each functioned independently as CBD in XynA and their binding capacity was additive. Although the Ruminococcus albus endoglucanase EgIV that was joined to CBDs of XynA acquired cellulose-binding ability, the substrate specificity of EgIV was not altered in the presence or absence of CBDs.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
Sakka K,Takada G,Karita S,Ohmiya Kdoi
10.1111/j.1749-6632.1996.tb40565.xsubject
Has Abstractpub_date
1996-05-15 00:00:00pages
241-51eissn
0077-8923issn
1749-6632journal_volume
782pub_type
杂志文章abstract：:Patients with renal impairment have an increased risk for cardiovascular disease, which may be the result of advanced glycation end products (AGEs). The aim of this study was to investigate the levels of AGE peptides in relation to kidney function and to study the relationship of AGE peptides with endothelial function...
journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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更新日期：1999-11-18 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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doi：10.1111/j.1749-6632.1976.tb47020.x
更新日期：1976-01-01 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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doi：10.1111/j.1749-6632.1991.tb00218.x
更新日期：1991-01-01 00:00:00
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journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1749-6632.1981.tb12754.x
更新日期：1981-02-27 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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更新日期：1979-01-01 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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journal_title：Annals of the New York Academy of Sciences
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abstract：:Evolutionary psychology seeks to understand the functional design underlying psychological processes and behavior. Theories of selection pressures developed within evolutionary biology are key components of this understanding. Because past selection pressures responsible for current design cannot be directly observed,...
journal_title：Annals of the New York Academy of Sciences
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doi：10.1111/j.1749-6632.2000.tb06615.x
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abstract：:CISD2, the causative gene for Wolfram syndrome 2 (WFS2), is an evolutionarily conserved novel gene. Recently, we have demonstrated that CISD2 is involved in mammalian lifespan control; this work also establishes WFS2 as a mitochondria-mediated disorder and effectively links CISD2 gene function, mitochondrial integrity...
journal_title：Annals of the New York Academy of Sciences
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更新日期：2010-07-01 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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doi：10.1111/j.1749-6632.1995.tb17409.x
更新日期：1995-03-27 00:00:00
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journal_title：Annals of the New York Academy of Sciences
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更新日期：2012-10-01 00:00:00
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journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1749-6632.1977.tb29433.x
更新日期：1977-03-11 00:00:00
abstract：:The effects of visual field changes and ocular hypertension on visual evoked potentials were investigated by photopic ERG and by luminance and pattern-reversal EPs on 116 glaucomatous and on 7 normal eyes. The problem was approached by way of four investigations: Firstly, which nerve structures are affected by glaucom...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章
doi：10.1111/j.1749-6632.1982.tb50794.x
更新日期：1982-01-01 00:00:00
abstract：:Older age is associated with functional declines throughout the body, including some aspects of cognitive performance. While dementia develops in only some elderly individuals, declines in cognitive functioning have an impact on daily living for many others. There are individual differences in age-related cognitive ch...
journal_title：Annals of the New York Academy of Sciences
pub_type： 杂志文章,评审
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更新日期：2005-12-01 00:00:00
abstract：:The psychostimulant 3,4-,ethylenedioxymethamphetamine (MDMA, "ecstasy") is known to induce hyperthermia and alterations in neurochemical metabolism in the CNS. However, the detailed cellular or molecular mechanisms behind MDMA-induced neurotoxicity are still not well known. Since MDMA induces profound hyperthermia tha...
journal_title：Annals of the New York Academy of Sciences
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