Abstract:
:Long-term exposure to high manganese (Mn) levels can lead to Parkinson-like neurological disorders. Molecular mechanisms underlying Mn cytotoxicity have been not defined. It is known that Mn induces apoptosis in PC12 cells and that this involves the activation of some signal transduction pathways. Although the role of phospholipids in apoptosis and signal transduction is well-known, the membrane phospholipid component in Mn-related damage has not yet been investigated. Phosphatidylserine (PS) facilitates protein translocation from cytosol to plasma membrane and PS exposure on the cell surface allows macrophage recognition of apoptotic cells. This study investigates the effects of MnCl2 on PS metabolism in PC12 cells, relating them to those on cell apoptosis. Apoptosis induction decreased PS radioactivity of PC12 cells incubated with radioactive serine. MnCl2 reduced PS radioactivity even under conditions that did not affect cell viability or PS exposure, suggesting that the effects on PS metabolism may represent an early event in cell apoptosis. Thus the latter conditions that also induced a greater PS decarboxylation were utilized for further investigating on the effects on PS synthesis, by measuring the activity and expression of PS-synthesizing enzymes, in cell lysates and in total cellular membranes (TM). Compared with corresponding controls, enzyme activity of MnCl2-treated cells was lower in cell lysates and greater in TM. Evaluating the expression of two isoforms of PS-synthesizing enzyme (PSS), PSSII was increased both in cell lysate and TM, while PSSI was unchanged. MnCl2 addition to control cell lysate reduced enzyme activity. These results suggest Mn plays a dual role on PS synthesis. Once inside the cell, Mn inhibits the enzyme/s, thus accounting for reduced PS synthesis in lysates and intact cells. On the other hand, it increases PSSII expression in cell membranes. The possibility that this occurs to counteract the direct effects of Mn ions on enzyme activity cannot be excluded. The effects on membrane enzyme activity and expression may also participate to PS exposure, observed at longer periods of treatment, by increasing membrane PS content.
journal_name
Neurotoxicologyjournal_title
Neurotoxicologyauthors
Ferrara G,Gambelunghe A,Mozzi R,Marchetti MC,Migliorati G,Muzi G,Buratta Sdoi
10.1016/j.neuro.2013.07.006subject
Has Abstractpub_date
2013-12-01 00:00:00pages
25-34eissn
0161-813Xissn
1872-9711pii
S0161-813X(13)00120-4journal_volume
39pub_type
杂志文章相关文献
NEUROTOXICOLOGY文献大全abstract::Many uncertainties remain today concerning effects of lead on the nervous system, particularly in regard to levels of lead exposure causing minimal brain dysfunction in children. Past studies in children have failed to define adequately the nature of the effect, the critical period and duration of exposure and the rol...
journal_title:Neurotoxicology
pub_type: 杂志文章,评审
doi:
更新日期:1984-10-01 00:00:00
abstract::The effects of exposure to volatile organic compounds (VOCs), which are of concern to the EPA, are poorly understood, in part because of insufficient characterization of how human exposure duration impacts VOC effects. Two inhalation studies with multiple endpoints, one acute and one subchronic, were conducted to seek...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2015.09.001
更新日期:2015-12-01 00:00:00
abstract::Administration of 0.3% aluminum in drinking water elevated serum aluminum concentrations 8-fold in rats. Further, chronic treatment with aluminum for 2-3 mon, in both developing and adult rats, significantly decreased the levels of MAP-2 in brain, as determined by quantitative immunoblot analysis. Aluminum treatment a...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:
更新日期:1992-07-01 00:00:00
abstract::Acrylonitrile (ACN) is a widely used chemical in the production of plastics, resin, nitrile, acrylic fibers, synthetic rubber, and acrylamide. ACN is considered a Group 2B possible carcinogen in humans and is known to cause gliomas in rats. These gliomas are predominantly composed of microglia and not astrocytes. Inte...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2017.09.013
更新日期:2017-12-01 00:00:00
abstract::A growing body of experimental and clinical literature indicates an association between Gaucher disease and parkinsonism, raising the possibility that convergent mechanisms may contribute to neurodegeneration in these disorders. The aim of this study was to determine whether there is a relationship between alpha-synuc...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2009.06.009
更新日期:2009-11-01 00:00:00
abstract::We have developed an estrogen sensitive reporter gene assay and report here on its responsiveness to steroid hormones and several estrogenic contaminants, including pesticides and polychlorinated biphenyls (PCBs), GH4C1 rat pituitary cells were stably transfected with the reported gene ERE-TK-Luc which contains a luci...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::We have evidence that methamphetamine (METH)-induced neuronal death is morphologically necrotic, not apoptotic, as is currently believed, and that electrographic seizures may be responsible. We administered 40mg/kg i.p. to 12 male C57BL/6 mice and monitored EEGs continuously and rectal temperatures every 15min, keepin...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2015.11.001
更新日期:2016-01-01 00:00:00
abstract::Chronic manganese (Mn) exposure can lead to neuroinflammation and neurological deficit, which resemble idiopathic Parkinson's disease (IPD). However, the precise mechanisms underlying Mn exposure-induced neurotoxicity remain incompletely understood. Microglia can become hyperactivated and plays a vital role in neuroin...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2017.04.001
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Among the adverse health effects of exposure to pesticides, an association with diabetes has been reported. However, there is a lack of epidemiologic studies on the health effects of exposure to pesticides, particularly investigating the association between occupational pesticide exposure and diabetes preval...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2018.10.007
更新日期:2019-01-01 00:00:00
abstract::Although synergism between pesticides has been widely documented, the physiological mechanisms by which an insecticide synergizes another remains unclear. Toxicological and electrophysiological studies were carried out on two susceptible pest species (the mosquito Culex quinquefasciatus and the cockroach Periplaneta a...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2006.01.011
更新日期:2006-07-01 00:00:00
abstract::Nonylphenols (NP) are endocrine-disruptors known to be widely present in our environment. This study evaluated the effects of 4-n-NP on neurobehavioral development and memory capacity after perinatal exposure on the offspring rats. Dams were gavaged with 4-n-NP (0, 50 and 200mg/kg/day) from gestational day 5 to postna...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2014.07.002
更新日期:2014-09-01 00:00:00
abstract::The chelating agent, CaNa2EDTA, has proven useful for both the diagnosis and treatment of lead poisoning. The EDTA lead-mobilization test has demonstrated the presence of excessive body lead stores when blood concentrations were "normal." The EDTA lead-mobilization test is, however, impractical because it requires inj...
journal_title:Neurotoxicology
pub_type: 杂志文章,评审
doi:
更新日期:1992-01-01 00:00:00
abstract::The in vitro and vivo effects of lead on the NMDA-receptors in adult and juvenile mice were studied by means of receptor binding assays. Adult female NMRI-mice received 100 and 1,000 ppm lead as nitrate in their drinking water for 30 and 90 days. Perinatal exposure was achieved by treating gestating mice from the 5th ...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract::The organophosphorus insecticide chlorpyrifos (CPF) is suspected to cause developmental neurotoxicity in children leading to long term effects. Developmental exposure of rat pups to CPF at low levels disrupts degradation of the brain endocannabinoids through the inhibition of fatty acid amide hydrolase (FAAH) and decr...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2020.01.002
更新日期:2020-03-01 00:00:00
abstract::Clinical use of vincristine (VCR), an effective chemotherapeutic agent, has been limited due to its peripheral neuropathy toxicity. Sumatriptan, which is an anti-migraine agent is a specific agonist for 5-hydroxytryptamine 1B, 1D (5HT1B, 1D) receptors. Several studies have shown that sumatriptan exerts anti-inflammato...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2018.06.012
更新日期:2018-07-01 00:00:00
abstract::Environmental exposure to methylmercury (MeHg) during development is of concern because it is easily incorporated in children's body both pre- and post-natal, it acts at several levels of neural pathways (mitochondria, cytoskeleton, neurotransmission) and it causes behavioral impairment in child. We evaluated the effe...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2016.05.018
更新日期:2017-03-01 00:00:00
abstract::In the past six years, our research group has investigated the relationship between lead exposure and mental and behavioural development. This has been carried out through studies in children and animals. Earlier studies in children have shown that associations might be expected between environmental exposure to lead ...
journal_title:Neurotoxicology
pub_type: 杂志文章,评审
doi:
更新日期:1986-07-01 00:00:00
abstract::Recently, it has been proposed that a variety of environmental disruptors (EDs) disturb the neonatal development of the brain in mammals because of their lipophilic characteristics. Therefore, the synergism of these lipophilic compounds is important when evaluating the risk from EDs. In mouse cerebellar granule cells ...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2004.07.008
更新日期:2005-01-01 00:00:00
abstract::Chlordecone (0.1 to 10 mg) or corn oil vehicle was injected into Japanese quail eggs on day 1 of incubation. Higher doses (0.5-10 mg per egg) produced tremor and ataxia at hatching and dose-related decreases in hatchability and survivability. Doses lower than 0.25 mg per egg had no effects. Gonad weights were not affe...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract:BACKGROUND:The organophosphate insecticide chlorpyrifos (CPF), widely used for agricultural purposes, has been linked to neurodevelopmental deficits. Possible motor effects at low to moderate levels of exposure have not been evaluated. METHODS:Prenatal exposure to CPF was measured in umbilical cord blood in a sample o...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2015.09.004
更新日期:2015-12-01 00:00:00
abstract::Selenium (Se) supplementation in the nutritionally relevant range counteracts methylmercury (MeHg) toxicity. Since Se tends to be abundant in fish, MeHg exposures alone may not provide an accurate index of risk from fish consumption. Molar ratios of MeHg:Se in the diets and Hg:Se in tissues of exposed individuals may ...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2008.07.007
更新日期:2008-09-01 00:00:00
abstract::Nickel, a known occupational/environmental hazard, may cross the placenta and reach appreciable concentrations in various fetal organs, including the brain. The aim of this study was to investigate whether nickel interferes with the process of neuronal differentiation. Following a 4 week treatment with retinoic acid (...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2015.01.001
更新日期:2015-03-01 00:00:00
abstract::Physiological methods that can be similarly recorded in humans and animals have a major role in sensory toxicology, as they provide a bridge between human sensory perception data and the molecular and cellular data obtained in animal studies. Vestibular toxicity research lags well behind other sensory systems in many ...
journal_title:Neurotoxicology
pub_type: 杂志文章,评审
doi:10.1016/j.neuro.2018.02.003
更新日期:2018-05-01 00:00:00
abstract::For the diagnosis of patients suspected of chronic solvent-induced encephalopathy (CSE), it would be helpful if the applied cognitive tests show a characteristic profile of impairment in this disease. We investigated the existence of such a profile. In 1997-2006 two expert teams in The Netherlands systematically exami...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2012.06.005
更新日期:2012-08-01 00:00:00
abstract::MPP(+) (1-methyl-4-phenylpyridinium; the active metabolite of the neurotoxin MPTP (1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine)) depletes dopamine (DA) content and elicits cell death in PC12 cells. However, the mechanism of MPP(+)-induced neurotoxicity is still unclear. In this study, the dose response and time-cours...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2004.12.008
更新日期:2005-08-01 00:00:00
abstract::Extracellular acetylcholine (ACh) levels were determined, by intracranial microdialysis, in medial septum, amygdala and hippocampus (CA1, CA3, dentate gyrus) of rats during seizures induced by systemic administration of soman (pinacolyl methylphosphonofluoridate), a potent inhibitor of acetylcholinesterase (AChE). In ...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::An injection of trimethyltin (TMT) into adult male Wistar rats produced a transient hyperirritability and aggressive behavior. When these effects of the drug subsided, the rats were still markedly impaired in performance of a spatial memory task examined in a watermaze. Activity of neurons in the hippocampus of these ...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::The ubiquitous functional roles of brain catecholamines have led to the notion that in vitro neurochemical changes in these systems may predict neurotoxicity. Conversely, others have argued that the appropriate use of neurochemical methods is for testing specific hypotheses that are developed based on observed phenome...
journal_title:Neurotoxicology
pub_type: 杂志文章,评审
doi:
更新日期:1987-04-01 00:00:00
abstract::The present study was envisaged to investigate the neuroprotective potential of Allium cepa (A. cepa) in aluminium chloride induced neurotoxicity. Aluminium chloride (50 mg/kg/day) was administered orally in mice supplemented with different doses of A. cepa hydroethanolic extract for a period of 60 days. Various behav...
journal_title:Neurotoxicology
pub_type: 杂志文章
doi:10.1016/j.neuro.2015.04.007
更新日期:2015-07-01 00:00:00
abstract::Mice who were exposed to phenobarbital prenatally (B mice) had at adulthood deficits in the hippocampal eight-arm maze, spontaneous alternations, and water maze behaviors. Morphological studies revealed neuronal losses in the hippocampus. The surviving neurons had reductions from control in the number of dendritic bra...
journal_title:Neurotoxicology
pub_type: 杂志文章,评审
doi:
更新日期:1989-10-01 00:00:00