Abstract:
BACKGROUND:Enhanced cell expression of MAdCAM-1 is critical in tissue recruitment of lymphocytes in response to stimuli expressing the α4β7 integrin. MAdCAM-1 is well characterized in gut mucosa with emerging evidence of hepatic expression. AIMS:(i) Compare quantitative/semi-quantitatively MAdCAM-1 expression in relation to early and advanced liver diseases (ii) Define the fine structure of vascular plexuses/lymphatics in the portal tract on which MAdCAM-1 is expressed. METHODS:Using alkaline phosphatase anti-alkaline phosphatase methodology on paraffin embedded tissue sections (n=28) from cirrhotic individuals who underwent orthotopic liver transplant, we evaluated MAdCAM-1 expression and compared with pre-cirrhotic, fulminant hepatitis B, and non-cirrhotic portal hypertension tissue sections. The positive controls included normal colon tissue with negative controls without primary antibody and isotype-matched purified IgG. We developed a real time PCR to quantify levels of MAdCAM-1 mRNA in our samples. RESULTS:MAdCAM-1 was expressed in 27/28 of the cirrhotic sections, localized primarily to septal areas within (i) endothelium of the peribiliary vascular plexus (PBP) (ii) lymphoid aggregates, with absence from normal, non-cirrhotic portal hypertension and pre-cirrhotic livers. There was significant upregulation of MAdCAM-1 mRNA in cirrhosis (p<0.011), consistent with immunohistochemical analysis. CONCLUSIONS:MAdCAM-1 is up-regulated in cirrhosis with expression on PBP and lymphoid aggregates. MAdCAM-1 is likely to contribute to the localization and recruitment of α4β7 lymphocytes during the pathogenesis of cirrhosis. MAdCAM-1 could be a useful marker of advanced liver disease. Further studies with respect to the expression of MAdCAM-1 in the presence of reversible and non-reversible stages of liver disease may be of merit.
journal_name
Dig Dis Scijournal_title
Digestive diseases and sciencesauthors
Ala A,Brown D,Khan K,Standish R,Odin JA,Fiel MI,Schiano TD,Hillan KJ,Rahman SA,Hodgson HJ,Dhillon APdoi
10.1007/s10620-013-2755-1subject
Has Abstractpub_date
2013-09-01 00:00:00pages
2528-41issue
9eissn
0163-2116issn
1573-2568journal_volume
58pub_type
杂志文章abstract:BACKGROUND AND AIMS:Capsule endoscopy (CE) has the problem that lumen visualization is impaired by bubbles, bile, and debris. The benefits of bowel preparation are still controversial and the best method remains to be determined. The objective of this study was to evaluate the effect of the method of bowel preparation ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章,随机对照试验
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更新日期:2011-06-01 00:00:00
abstract::Gastrin-secreting tumors have been identified in ectopic locations including the ovary; the mechanisms regulating gastrin gene expression, its distribution, and signaling pathways in these ectopic tissues are not known. The purpose of our present study was to determine: (1) whether the gastrin gene and peptide could b...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01297450
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01296714
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journal_title:Digestive diseases and sciences
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doi:10.1007/BF02087561
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章,评审
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journal_title:Digestive diseases and sciences
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更新日期:2015-06-01 00:00:00
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/b:ddas.0000017451.48031.b6
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journal_title:Digestive diseases and sciences
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更新日期:2011-06-01 00:00:00
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journal_title:Digestive diseases and sciences
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journal_title:Digestive diseases and sciences
pub_type: 临床试验,杂志文章
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF02065531
更新日期:1995-06-01 00:00:00
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1012791832730
更新日期:2001-12-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1007/s10620-017-4697-5
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journal_title:Digestive diseases and sciences
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pub_type: 临床试验,杂志文章
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更新日期:2008-10-01 00:00:00
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更新日期:2015-12-01 00:00:00
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-010-1532-7
更新日期:2011-03-01 00:00:00
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journal_title:Digestive diseases and sciences
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1993-03-01 00:00:00
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00
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journal_title:Digestive diseases and sciences
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更新日期:1992-11-01 00:00:00
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-014-3438-2
更新日期:2015-07-01 00:00:00
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journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1018875718392
更新日期:1998-09-01 00:00:00