Abstract:
:Congenital diseases caused by abnormal development of the cranial neural crest usually present craniofacial malformations and heart defects while the precise mechanism is not fully understood. Here, we show that the zebrafish eif3ba mutant caused by pseudo-typed retrovirus insertion exhibited a similar phenotype due to the hypogenesis of cranial neural crest cells (NCCs). The derivatives of cranial NCCs, including the NCC-derived cell population of pharyngeal arches, craniofacial cartilage, pigment cells and the myocardium derived from cardiac NCCs, were affected in this mutant. The expression of several neural crest marker genes, including crestin, dlx2a and nrp2b, was specifically reduced in the cranial regions of the eif3ba mutant. Through fluorescence-tracing of the cranial NCC migration marker nrp2b, we observed reduced intensity of NCC-derived cells in the heart. In addition, p53 was markedly up-regulated in the eif3ba mutant embryos, which correlated with pronounced apoptosis in the cranial area as shown by TUNEL staining. These findings suggest a novel function of eif3ba during embryonic development and a novel level of regulation in the process of cranial NCC development, in addition to providing a potential animal model to mimic congenital diseases due to cranial NCC defects. Furthermore, we report the identification of a novel transgenic fish line Et(gata2a:EGFP)pku418 to trace the migration of cranial NCCs (including cardiac NCCs); this may serve as an invaluable tool for investigating the development and dynamics of cranial NCCs during zebrafish embryogenesis.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Xia Z,Tong X,Liang F,Zhang Y,Kuok C,Zhang Y,Liu X,Zhu Z,Lin S,Zhang Bdoi
10.1016/j.ydbio.2013.06.009keywords:
["Cardiac neural crest","Cranial neural crest","Zebrafish","eif3ba","nrp2b","p53"]subject
Has Abstractpub_date
2013-09-01 00:00:00pages
83-96issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(13)00326-6journal_volume
381pub_type
杂志文章abstract::Hes3 belongs to the Hes basic helix-loop-helix family of transcriptional repressors that play central roles in maintaining progenitor cells and regulating binary cell fate decisions in the embryo. During Xenopus laevis development, hes3 is expressed in the embryonic ectoderm in a horseshoe shape domain at the edge of ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.07.011
更新日期:2018-10-01 00:00:00
abstract::Fibronectin (Fn) plays an important part in the branching morphogenesis of salivary gland, lung, and kidney. Here, we examine the effect of the conditional knockout of Fn in the mammary epithelium [Fn(MEp-/-)] on postnatal mammary gland development, using Cre-loxP-mediated gene knockout technology. Our data show that ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.07.001
更新日期:2010-10-01 00:00:00
abstract::Actin dynamics are necessary at multiple steps in the formation of multinucleated muscle cells. BAR domain proteins can regulate actin dynamics in several cell types, but have been little studied in skeletal muscle. Here, we identify novel functions for the N-BAR domain protein, Bridging integrator 3 (Bin3), during my...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.07.004
更新日期:2013-10-01 00:00:00
abstract::High osmolarity, bound water, and hydrostatic pressure contribute to notochord mechanics and its morphogenesis into the nucleus pulposus (NP) compartment of the intervertebral disc. Indeed, the osmoadaptive transcription factor, nuclear factor of activated T-cells 5 (NFAT5 aka TonEBP), is robustly expressed by residen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.07.004
更新日期:2019-11-15 00:00:00
abstract::To examine the role of postsynaptic activity in regulating the rate of neuromuscular synapse elimination, contractile activity of neonatal rabbit soleus muscles was decreased by chronic superfusion of alpha-bungarotoxin (alpha-BGT) over their surfaces. Superfusion was begun at 6 days postnatal and continued for a vari...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80009-0
更新日期:1989-02-01 00:00:00
abstract::When a posterior fragment of the chick's marginal zone (PM) was exchanged with equal sized lateral marginal zone fragment (LM), of the same blastoderm, its capacity to initiate an ectopic primitive streak (PS) was found to be both size and stage dependent. Good correlation was demonstrated between the areas of PM frag...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90091-2
更新日期:1989-07-01 00:00:00
abstract::The diaphragm is a mammalian skeletal muscle essential for respiration and for separating the thoracic and abdominal cavities. Development of the diaphragm requires the coordinated development of muscle, muscle connective tissue, tendon, nerves, and vasculature that derive from different embryonic sources. However, de...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.04.010
更新日期:2018-08-15 00:00:00
abstract::Satellite cells, also known as muscle stem cells, are responsible for skeletal muscle growth and repair in mammals. Pax7 and Pax3 transcription factors are established satellite cell markers required for muscle development and regeneration, and there is great interest in identifying additional factors that regulate sa...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.03.004
更新日期:2017-04-15 00:00:00
abstract::Catecholaminergic cells are transiently present during development of the fetal murine bowel. These transient catecholaminergic (TC) cells appear at Day E10, but by Day E13 can no longer be detected. In order to evaluate the hypothesis that these cells are the precursors of enteric neurons, we investigated the possibi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90217-0
更新日期:1989-03-01 00:00:00
abstract::A mammalian embryo experiences the first cell segregation at the blastocyst stage, in which cells giving form to the embryo are sorted into two lineages; trophectoderm (TE) and inner cell mass (ICM). This first cell segregation process is governed by cell position-dependent Hippo signaling, which is a phosphorylation ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.09.004
更新日期:2020-12-01 00:00:00
abstract::The Rho family of small GTPases has been implicated in many neurological disorders including mental retardation, but whether they are involved in primary microcephaly (microcephalia vera) is unknown. Here, we examine the role of Rac1 in mammalian neural progenitors and forebrain development by a conditional gene-targe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.10.023
更新日期:2009-01-01 00:00:00
abstract::Pioneer axons in insect legs are experimentally accessible model systems for the molecular identification and cellular localization of guidance cues regulating the path of axon growth. A detailed study of the Fe2 pioneer axons in the legs of the cockroach was performed to examine the diversity of guidance mechanisms. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8704
更新日期:1997-10-15 00:00:00
abstract::During limb development, Sonic hedgehog (SHH) and HOX proteins are considered among the most important factors regulating digit number and identity. SHH signaling prevents the processing of GLI3 into a short form that functions as a strong transcriptional repressor. Gli3 mutant limbs are characterized by a severe poly...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.07.023
更新日期:2007-10-15 00:00:00
abstract::The protein tyrosine phosphatase PTP1, which mediates reversible phosphorylation on tyrosine, has been shown to play an important regulatory role during Dictyostelium development. Mutants lacking PTP1 develop more rapidly than normal, while strains that overexpress PTP1 display aberrant morphology. However, the signal...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0171
更新日期:2001-04-01 00:00:00
abstract::Chromatin insulators orchestrate gene transcription during embryo development and cell differentiation by stabilizing interactions between distant genomic sites. Mutations in genes encoding insulator proteins are generally lethal, making in vivo functional analyses of insulator proteins difficult. In Drosophila, howev...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.03.024
更新日期:2015-07-01 00:00:00
abstract::We have investigated the ability of growing dictyate oocytes and early preimplantation embryos of the mouse to process extrachromosomal DNA molecules with free ends by intranuclearly microinjecting DNA fragments containing a region of homology of various extent at either the 5' or 3' terminus. Homologous recombination...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0199
更新日期:2001-05-01 00:00:00
abstract::Development and plasticity of synapses are brought about by a complex interplay between various signaling pathways. Typically, either changing the number of synapses or strengthening an existing synapse can lead to changes during synaptic plasticity. Altering the machinery that governs the exocytosis of synaptic vesic...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.09.025
更新日期:2013-12-01 00:00:00
abstract::In the present study, we have investigated the role of tyrosine kinase activity during early retinal development in Xenopus laevis. The protein tyrosine kinase (PTK) inhibitors lavendustin A and genistein were used to determine the possible role of tyrosine kinase activity during retinal development in vivo and in vit...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.10.006
更新日期:2004-02-01 00:00:00
abstract::Ephrin-B2, a member of the Eph/ephrin family of cell signaling molecules, has been implicated in the guidance of cranial and trunk neural crest cells (NCC) and development of the branchial arches(BA), but detailed examination in mice has been hindered by embryonic lethality of Efnb2 null loss of function due to a requ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.08.021
更新日期:2015-10-15 00:00:00
abstract::Notch signaling has a fundamental role in stem cell maintenance and in cell fate choice in the intestine of different species. Canonically, Notch signaling represses the expression of transcription factors of the achaete-scute like (ASCL) or atonal related protein (ARP) families. Identifying the ARP/ASCL genes express...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.01.011
更新日期:2013-04-15 00:00:00
abstract::Monoclonal antibodies highly selective for developmentally regulated antigens present in the cornea (Zak and Linsenmayer, Dev. Biol. 99, 373-381, 1983) have been used to immunohistochemically evaluate differentiation in intact chick corneas cultured on the chorioallantoic membrane (CAM) of host embryos. One antibody i...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90047-8
更新日期:1985-04-01 00:00:00
abstract::The current academic and business landscape does not provide a clear path for doctoral trainees graduating. Moreover, the fundamental need of the hour is diversification of the workforce where PhD graduates need to be trained to do more beyond basic scientific research. One of the possible paths that is now becoming p...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.10.025
更新日期:2020-03-01 00:00:00
abstract::Plants often display a high competence for regeneration under stress conditions. Signals produced in response to various types of stress serve as critical triggers for de novo organogenesis, but the identity of these signaling molecules underlying cellular reprogramming are largely unknown. We previously identified an...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.07.006
更新日期:2018-10-01 00:00:00
abstract::Urodeles begin gastrulation with much of their presumptive mesoderm in the superficial cell layer, all of which must move into the deep layers during development. We studied the morphogenesis of superficial mesoderm in the urodeles Ambystoma maculatum, Ambystoma mexicanum, and Taricha granulosa. In all three species, ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0718
更新日期:2002-08-15 00:00:00
abstract::The gastrointestinal (GI) tract of metazoans is lined by a series of regionally distinct epithelia. To maintain structure and function of the GI tract, regionally diversified differentiation of somatic stem cell (SC) lineages is critical. The adult Drosophila midgut provides an accessible model to study SC regulation ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.08.027
更新日期:2016-11-15 00:00:00
abstract::To understand cell interactions during induction of skin appendages, we studied the roles of adhesion molecules N-CAM, tenascin, integrin, and fibronectin during feather development. Tenascin appeared in a periodic pattern on epithelia and was so far the earliest molecule detected in placodes. Three placode domains we...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90009-6
更新日期:1992-03-01 00:00:00
abstract::Botryllus schlosseri is a colonial marine urochordate in which all adult organisms (called zooids) in a colony die synchronously by apoptosis (programmed cell death) in cyclical fashion. During this death phase called takeover, cell corpses within the dying organism are engulfed by circulating phagocytic cells. The "o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0772
更新日期:2002-09-15 00:00:00
abstract::The propagation of pluripotential mouse embryonic stem (ES) cells is sustained by leukemia inhibitory factor (LIF) or related cytokines that act through a common receptor complex comprising the LIF receptor subunit (LIF-R) and the signal transducer gp130. However, the findings that embryos lacking LIF-R or gp130 can d...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9026
更新日期:1998-11-01 00:00:00
abstract::Morphogenesis of the Drosophila tracheal system relies on different signalling pathways that have distinct roles in specifying both the migration of the tracheal cells and the particular morphological features of the primary branches. The current view is that the tracheal cells are initially specified as an equivalent...
journal_title:Developmental biology
pub_type: 杂志文章
doi:
更新日期:2002-10-15 00:00:00
abstract::In the full grown oocyte of Xenopus laevis, a sodium permeability can be induced by depolarization to positive potential with current injection. Voltage-clamp analysis has shown that depolarization causes a long-lasting modification of the membrane during which voltage-gated sodium channels become functional. In the p...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90302-0
更新日期:1983-10-01 00:00:00