Physostigmine reverses disturbances of the intestinal microcirculation during experimental endotoxemia.

Abstract:

:Intestinal microcirculatory disturbances play an important role in the pathophysiology of sepsis. A neural anti-inflammatory pathway has been suggested as a potential target for therapy that may dampen systemic inflammation. The aim of this study is to investigate the effects of physostigmine, a cholinesterase inhibitor, on the intestinal microcirculation and vascular contractility in experimental endotoxemia. Endotoxemia was induced in Lewis rats by intravenous lipopolysaccharide (LPS) administration. Animals were treated with either physostigmine or saline (control) following LPS challenge. The intestinal microcirculation, including leukocyte-endothelial interaction, functional capillary density (FCD) and non-perfused capillary density (NCD), was examined by intravital microscopy (IVM) 2 hours after LPS administration. The impact of physostigmine on vascular contractility of rat aortic rings was examined by in vitro myography. Physostigmine significantly reduced the number of adhering leukocytes in intestinal submucosal venules (V1 venules: -61%, V3 venules: -36%) of LPS animals. FCD was significantly increased by physostigmine treatment (circular muscle layer: +180%, longitudinal muscle layer: +162%, mucosa: +149%). Low concentrations of physostigmine produced significant contraction of aortic ring preparations, whereas high concentrations produced relaxation. In conclusion, physostigmine treatment significantly improved the intestinal microcirculation in experimental endotoxemia by reducing leukocyte adhesion and increasing FCD.

authors

Zhou J,Pavlovic D,Rüb J,Masur S,Spassov A,Whynot S,Hung O,Kern H,Saleh Abdo I,Shukla R,Cerny V,Lehmann C

doi

10.3233/CH-131743

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

273-84

issue

3

eissn

1386-0291

issn

1875-8622

pii

J75X54X2L1282P4G

journal_volume

56

pub_type

杂志文章
  • Effects of exercise training on blood rheology: a meta-analysis.

    abstract::Regular exercise has been reported to improve blood rheology, but all the studies investigating this issue included a reduced number of subjects, and thus it was logic to perform a meta-analysis of them in order to better characterize this physiological effect. Research was handed on Medline from 1950 to 2010. Studies...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章,meta分析

    doi:10.3233/CH-2011-1469

    authors: Romain AJ,Brun JF,Varlet-Marie E,Raynaud de Mauverger E

    更新日期:2011-01-01 00:00:00

  • Importance of methodological standardization for the ektacytometric measures of red blood cell deformability in sickle cell anemia.

    abstract::Red blood cell (RBC) deformability is severely decreased in patients with sickle cell anemia (SCA), which plays a role in the pathophysiology of the disease. However, investigation of RBC deformability from SCA patients demands careful methodological considerations. We assessed RBC deformability by ektacytometry (LORR...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-151979

    authors: Renoux C,Parrow N,Faes C,Joly P,Hardeman M,Tisdale J,Levine M,Garnier N,Bertrand Y,Kebaili K,Cuzzubbo D,Cannas G,Martin C,Connes P

    更新日期:2016-01-01 00:00:00

  • Relationships between von Willebrand factor and hemorheology in sportsmen.

    abstract::It is well established that exercise performance in athletes is related to improved blood fluidity. However, training effects on functional state of endothelium and relations of endothelial cell functions with hemorheology are poorly known. Circulating levels of von Willebrand factor may serve as a good marker of endo...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:

    authors: Melnikov AA,Vikulov AD,Bagrakova SV

    更新日期:2003-01-01 00:00:00

  • Macro- and microperfusion during application of a new compression system, designed for patients with leg ulcer and concomitant peripheral arterial occlusive disease.

    abstract:OBJECTIVE:To investigate macro- and microperfusion during 14 days of treatment with a new 2-layer compression system (3M™ Coban™ 2 Lite), designed for patients with leg ulcer and concomitant peripheral arterial occlusive disease. METHODS:A single-centre, open-label, prospective pilot study was performed with 15 subjec...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 临床试验,杂志文章

    doi:10.3233/CH-2012-1568

    authors: Jünger M,Haase H,Schwenke L,Bichel J,Schuren J,Ladwig A

    更新日期:2013-01-01 00:00:00

  • Generalized predictive equation for hematocrit by biological impedancemetry.

    abstract::Bioelectrical impedancemetry (BIA) has been used to evaluate hemorheological parameters from whole body measurements. In a previous study, we have determined a set of predictive equations for hematocrit, whole blood viscosity and plasma viscosity in athletes. In another previous study, we also found other predictive e...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:

    authors: Varlet-Marie E,Brun JF

    更新日期:2004-01-01 00:00:00

  • Doppler ultrasonography and contrast-enhanced ultrasonography to evaluate liver allograft discard: A pilot prospective study.

    abstract:BACKGROUND:Broad hemodynamic changes, is believed to have a profoundly damaging effect on donor livers after brain death (DBD) or cardiac death (DCD). It remains unclear whether Doppler ultrasonography (DUS) and contrast-enhanced ultrasonography (CEUS), the imaging modalities to evaluate perfusion, could provide more i...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-200950

    authors: Zhang HJ,Zheng BW,Gu SJ,Wu T,Wu LL,Lian YF,Tong G,Yi SH,Ren J

    更新日期:2020-09-08 00:00:00

  • Correlation analysis between different parameters of conduit artery and microvascular vasodilation.

    abstract::Impaired endothelial responsiveness to specific vasodilator stimuli has been used as a surrogate marker of cardiovascular risk. Multiple methods allow testing endothelial responses in both microvessels and conduit arteries, but it is still unclear whether there is a relationship in endothelial function between these t...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:

    authors: Gori T,Di Stolfo G,Sicuro S,Dragoni S,Lisi M,Parker JD,Forconi S

    更新日期:2006-01-01 00:00:00

  • Comparison of erythrocyte aggregability changes during ischemic and hemorrhagic stroke.

    abstract::The aim of this work was to perform a comparative investigation of erythrocyte aggregability changes in the peripheral and cerebral circulation during ischemic and hemorrhagic stroke. Subjects of the present study were patients with ischemic brain infarcts (14 patients) and with hemorrhagic stroke (21 patients) from t...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:

    authors: Bolokadze N,Lobjanidze I,Momtselidze N,Shakarishvili R,Mchedlishvili G

    更新日期:2006-01-01 00:00:00

  • Nitric oxide metabolites (nitrite and nitrate) in young patients with recent acute myocardial infarction.

    abstract::Nitric oxide (NO) has a role in the pathophysiology of acute and chronic cardiovascular events. We studied the plasma concentration of NO stable end products (nitrite and nitrate--NOx) in 43 patients aged<46 years, with recent acute myocardial infarction (AMI). The evaluation was effected at the initial stage, after 3...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:

    authors: Caimi G,Montana M,Calandrino V,Caruso M,Carollo C,Catania A,Lo Presti R

    更新日期:2008-01-01 00:00:00

  • Subcellular localization of the chemotherapeutic agent doxorubicin in renal epithelial cells and in tumor cells using correlative light and electron microscopy.

    abstract:BACKGROUND:Doxorubicin is a cytostatic drug from the group of anthracycline antibiotics that is widely used as a chemotherapeutic agent. Side effects of the active substance include cardiotoxicity and nephrotoxicity. Doxorubicin-treated renal epithelial cells and (sarcoma) tumors are examined by correlative light and e...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-199212

    authors: Asam C,Buerger K,Felthaus O,Brébant V,Rachel R,Prantl L,Witzgall R,Haerteis S,Aung T

    更新日期:2019-01-01 00:00:00

  • Shear-stress mediated nitric oxide production within red blood cells: A dose-response.

    abstract:BACKGROUND:Red blood cells (RBC) are exposed to varying shear stress while traversing the circulatory system; this shear initiates RBC-derived nitric oxide (NO) production. OBJECTIVE:The current study investigated the effect of varying shear stress dose on RBC-derived NO production. METHODS:Separated RBC were prepare...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-189412

    authors: Horobin JT,Watanabe N,Hakozaki M,Sabapathy S,Simmonds MJ

    更新日期:2019-01-01 00:00:00

  • Veno-active drugs in the management of chronic venous disease. An international consensus statement: current medical position, prospective views and final resolution.

    abstract:BACKGROUND:Veno-active drugs (VAD) have effects on edema and symptoms related to chronic venous disease (CVD), especially so-called venous pain. VAD's effectiveness, although well established, is regularly debated. OBJECTIVE:Our purpose was to select all randomized controlled trials (RCTs) and meta-analyses devoted to...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章,评审

    doi:

    authors: Ramelet AA,Boisseau MR,Allegra C,Nicolaides A,Jaeger K,Carpentier P,Cappelli R,Forconi S

    更新日期:2005-01-01 00:00:00

  • Exposure of human neutrophils to oxygen radicals causes loss of deformability, lipid peroxidation, protein degradation, respiratory burst activation and loss of migration.

    abstract::The effects of oxygen radical generating system of H(2)O(2) on neutrophil deformability, lipid peroxidation, protein degradation, chemotaxis, random migration and activation were studied in the presence and absence of selected anti-oxidants such as vitamin E and carbon monoxide. Hydrogen peroxide caused a significant ...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:

    authors: Abu-Khader AA,Bilto YY

    更新日期:2002-01-01 00:00:00

  • Alpha smooth muscle actin in the cycling ovary - an immunohistochemical study.

    abstract::In the ovary with its cyclically developing and regressing functional bodies and the associated intense neovascularisation and remodelling, alpha-smooth muscle actin (SMA) immunolocalisation has been frequently used as a marker to establish vessel hierarchy, in angiogenesis studies, or in studies characterising ovaria...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-2011-1448

    authors: Hirschberg RM,Plendl J,Kaessmeyer S

    更新日期:2012-01-01 00:00:00

  • The slow releasing hydrogen sulfide donor GYY4137 reduces neointima formation upon FeCl3 injury of the carotid artery in mice.

    abstract:INTRODUCTION:Neointima formation is closely linked to vascular stenosis and occurs after endothelial damage. Hydrogen sulfide is an endogenous pleiotropic mediator with numerous positive effects on the cardio vascular system. OBJECTIVE:This study evaluates the effect of the slow releasing hydrogen sulfide donor GYY413...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-190747

    authors: Grambow E,Klee G,Klar E,Vollmar B

    更新日期:2020-01-01 00:00:00

  • The influence of a multifunctional, polymeric biomaterial on the concentration of acute phase proteins in an animal model.

    abstract::The concentrations of the acute phase proteins alpha1-Acid Glycoprotein (AAG) and haptoglobin were determined in Sprague-Dawley-rats after implantation of a novel biodegradable multifunctional polymeric biomaterial for the reconstruction of a gastric wall defect (polymer group; n=42). For comparison, the concentration...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:

    authors: Rickert D,Scheithauer MO,Coskun S,Kelch S,Lendlein A,Franke RP

    更新日期:2007-01-01 00:00:00

  • Effects of storage duration and temperature of human blood on red cell deformability and aggregation.

    abstract::Blood samples used in hemorheological studies may be stored for a period of time, the effects of storage have yet to be fully explored. This study evaluated the effects of storage temperature (i.e., 4 degrees C or 25 degrees C) and duration on RBC deformability and aggregation for blood from healthy controls and from ...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-2009-1178

    authors: Uyuklu M,Cengiz M,Ulker P,Hever T,Tripette J,Connes P,Nemeth N,Meiselman HJ,Baskurt OK

    更新日期:2009-01-01 00:00:00

  • Evaluation of plasma cell mastitis with superb microvascular imaging.

    abstract:BACKGROUND:Plasma cell mastitis (PCM), a common type of mastitis often mimics malignancy clinically and radiologically. OBJECTIVE:The study was designed to explore the diagnostic value of superb microvascular imaging (SMI) in differentiating PCM from malignant breast lesions. METHODS:A total of 95 breast lesions unde...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-180468

    authors: Zhu YC,Zhang Y,Deng SH,Jiang Q,Shi XR,Feng LL

    更新日期:2019-01-01 00:00:00

  • Prospective evaluation of 4-D contrast-enhanced-ultrasound (CEUS) imaging in bladder tumors.

    abstract:PURPOSE:The evaluation of the potential clinical benefit of four-dimensional ultrasound (4D-US) in the assessment of bladder cancer (BC). MATERIAL AND METHODS:20 patients with indication for cystoscopy for suspicion of bladder cancer were prospectively included in this study. All patients underwent two-dimensional ult...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-199231

    authors: Jokisch F,Buchner A,Schulz GB,Grimm T,Weinhold P,Pfitzinger PL,Chaloupka M,Stief CG,Schlenker B,Clevert DA

    更新日期:2020-01-01 00:00:00

  • Intraductally applied contrast-enhanced ultrasound (IA-CEUS) for improved visualization of obstructive diseases of the salivary glands, primary results.

    abstract:PURPOSE:Obstructive diseases of the salivary glands are often based on Sialolithiasis; however, conventional radiological imaging or ultrasound does not provide a diagnosis in 5-10% of all cases. It was the aim of our study to examine the effectiveness and viability of an intraductal applied contrast-enhanced ultrasoun...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-2010-1298

    authors: Zengel P,Siedek V,Berghaus A,Clevert DA

    更新日期:2010-01-01 00:00:00

  • Lipid peroxidation and total antioxidant status in juvenile myocardial infarction.

    abstract::In a group of young subjects with acute myocardial infarction (AMI) (97 men and 8 women; mean age 39.6+/-5.5 years) we examined the thiobarbituric acid - reactive substances and the total antioxidant status at the initial stage and after 12 months. The same parameters were examined in a group of 55 control subjects. O...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:

    authors: Lo Presti R,Carollo C,Montana M,Canino B,Catania A,Caimi G

    更新日期:2008-01-01 00:00:00

  • Radiographic contrast media alterate the localization of actin/band4.9 in the membrane cytoskeleton of human erythrocytes.

    abstract::Different radiographic contrast media (RCM) were shown to induce morphological changes of blood cells (e.g. erythrocytes or thrombocytes) and endothelial cells. The echinocytic shape change of erythrocytes, particularly, affords alterations of the membrane cytoskeleton. The cytoskeleton plays a crucial role for the sh...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-141894

    authors: Franke RP,Scharnweber T,Fuhrmann R,Mrowietz C,Wenzel F,Krüger A,Jung F

    更新日期:2014-01-01 00:00:00

  • Contrast-enhanced ultrasonography (CEUS) using early dynamic in microcirculation for localization of pathological parathyroid glands: first-line or complimentary diagnostic modality?

    abstract:BACKGROUND:Contrast-enhanced ultrasonography (CEUS) represents a new diagnostic tool to localize pathological parathyroid glands. The aim of this study was to differentiate the capability of CEUS as first-line or complimentary modality for the detection of hyperfunctioning parathyroid glands. METHODS:Sixty patients wi...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-2011-1459

    authors: Hornung M,Jung EM,Stroszczynski C,Schlitt HJ,Agha A

    更新日期:2011-01-01 00:00:00

  • Effects of haemoglobin levels on the sublingual microcirculation in pregnant women.

    abstract::Anemia in pregnant women is associated with increased maternal and perinatal mortality and represents an important economic burden in many developing countries. Our goal was to evaluate the impact of anemia on the capillary network during pregnancy. Therefore, we compared microcirculatory parameters of anemic pregnant...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-162064

    authors: Sharawy N,Hussein A,Hossny O,Refaa A,Saka A,Mukhtar A,Whynot S,George R,Lehmann C

    更新日期:2016-11-25 00:00:00

  • Effect of a modified fibrate (Biniwas Retard) on hemorheological alterations in hyperlipemic patients.

    abstract::The effect of a binifibrate (Biniwas Retard, Wasserman) on the plasma lipids and hemorheological profile of 30 primary hyperlipemic patients was studied. Our results indicate that the patients under study had evident rheological alterations as well as the expected lipid alterations. Treatment with Biniwas (2 x 550 mg/...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Labios M,Martínez M,Vayá A,Gabriel F,Guiral V,Aznar J

    更新日期:1999-01-01 00:00:00

  • Increased hematocrit and reduced blood pressure following control of glycemia in diabetes.

    abstract::In this study we determine the effects of reducing blood glucose on mean arterial blood pressure (MAP) and hematocrit (Hct) in patients with type 2 diabetes who are not responding to conventional treatment in an intensive treatment program 1 year after initiation of treatment. Data on MAP, glucose and Hct was obtained...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 临床试验,杂志文章

    doi:

    authors: Salazar Vázquez BY,Salazar Vázquez MA,Venzor VC,Negrete AC,Cabrales P,Díaz JS,Intaglietta M

    更新日期:2008-01-01 00:00:00

  • Endothelial nitric oxide synthase expression in systemic and pulmonary circulation of streptozotocin induced diabetic rats: comparison using image analysis.

    abstract::To compare the level of endothelial nitric oxide synthase (eNOS) expression produced in heart and lung vascular tissue, the protein content was determined using Western blot analysis with the enhancement of image processing. Heart and lung extracts from 12 and 24 weeks from control (CON) and streptozotocin-induced dia...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:

    authors: Sridulyakul P,Chakraphan D,Bhattarakosol P,Patumraj S

    更新日期:2003-01-01 00:00:00

  • Protective effect of tetramethylpyrazine and salvianolic acid B on apoptosis of rat cerebral microvascular endothelial cell under high shear stress.

    abstract::Apoptosis induced by high shear stress has been reported for the dysfunction of various vascular endothelial cells. We investigated the protective effects of tetramethylpyrazine (TMP) and salvianolic acid B (SAB) from Chinese medicine on the shear-induced early and late stages of apoptosis in cultured rat cerebral mic...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:

    authors: Li M,Zhao MQ,Kumar Durairajan SS,Xie LX,Zhang HX,Kum WF,Goto S,Liao FL

    更新日期:2008-01-01 00:00:00

  • The effect of leukocyte-reduced platelet-rich plasma on the proliferation of autologous adipose-tissue derived mesenchymal stem cells.

    abstract::Clinical application of platelet-rich plasma (PRP) and stem cells has become more and more important in regenerative medicine during the last decade. However, differences in PRP preparations may contribute to variable PRP compositions with unpredictable effects on a cellular level. In the present study, we modified th...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-141920

    authors: Loibl M,Lang S,Brockhoff G,Gueorguiev B,Hilber F,Worlicek M,Baumann F,Grechenig S,Zellner J,Huber M,Valderrabano V,Angele P,Nerlich M,Prantl L,Gehmert S

    更新日期:2016-01-01 00:00:00

  • μCT based assessment of mechanical deformation of designed PTMC scaffolds.

    abstract:BACKGROUND:Advances in rapid-prototyping and 3D printing technologies have enhanced the possibilities in preparing designed architectures for tissue engineering applications. A major advantage in custom designing is the ability to create structures with desired mechanical properties. While the behaviour of a designed s...

    journal_title:Clinical hemorheology and microcirculation

    pub_type: 杂志文章

    doi:10.3233/CH-151931

    authors: Narra N,Blanquer SB,Haimi SP,Grijpma DW,Hyttinen J

    更新日期:2015-01-01 00:00:00