Abstract:
:Clinical application of platelet-rich plasma (PRP) and stem cells has become more and more important in regenerative medicine during the last decade. However, differences in PRP preparations may contribute to variable PRP compositions with unpredictable effects on a cellular level. In the present study, we modified the centrifugation settings in order to provide a leukocyte-reduced PRP and evaluated the interactions between PRP and adipose-tissue derived mesenchymal stem cells (ASCs).PRP was obtained after modification of three different centrifugation settings and investigated by hemogram analysis, quantification of protein content and growth factor concentration. ASCs were cultured in serum-free α-MEM supplemented with autologous 10% or 20% leukocyte-reduced PRP. Cell cycle kinetics of ASCs were analyzed using flow cytometric analyses after 48 hours.Thrombocytes in PRP were concentrated, whereas erythrocytes, and white blood cells (WBC) were reduced, independent of centrifugation settings. Disabling the brake further reduced the number of WBCs. A higher percentage of cells in the S-phase in the presence of 20% PRP in comparison to 10% PRP and 20% fetal calf serum (FCS) advocates the proliferation stimulation of ASCs.These findings clearly demonstrate considerable differences between three PRP separation settings and assist in safeguarding the combination of leukocyte-reduced PRP and stem cells for regenerative therapies.
journal_name
Clin Hemorheol Microcircjournal_title
Clinical hemorheology and microcirculationauthors
Loibl M,Lang S,Brockhoff G,Gueorguiev B,Hilber F,Worlicek M,Baumann F,Grechenig S,Zellner J,Huber M,Valderrabano V,Angele P,Nerlich M,Prantl L,Gehmert Sdoi
10.3233/CH-141920subject
Has Abstractpub_date
2016-01-01 00:00:00pages
599-614issue
4eissn
1386-0291issn
1875-8622pii
33651X3K6872G511journal_volume
61pub_type
杂志文章abstract:BACKGROUND:Doxorubicin is a cytostatic drug from the group of anthracycline antibiotics that is widely used as a chemotherapeutic agent. Side effects of the active substance include cardiotoxicity and nephrotoxicity. Doxorubicin-treated renal epithelial cells and (sarcoma) tumors are examined by correlative light and e...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-199212
更新日期:2019-01-01 00:00:00
abstract::Radiographic contrast media can lead to drastic changes of the morphology of erythrocytes. The change of the erythrocyte morphology is associated with a decreased deformability possibly resulting from distinctions in the loss of constituents of the membrane cytoskeleton. However, it is unclear whether there is an intr...
journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-141879
更新日期:2014-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-200977
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
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更新日期:1997-07-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 临床试验,杂志文章
doi:
更新日期:2008-01-01 00:00:00
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doi:10.3233/CH-2011-1459
更新日期:2011-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
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doi:10.3233/CH-2010-1357
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-131801
更新日期:2014-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
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doi:10.3233/CH-189125
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journal_title:Clinical hemorheology and microcirculation
pub_type: 临床试验,杂志文章
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journal_title:Clinical hemorheology and microcirculation
pub_type: 临床试验,杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1371
更新日期:2011-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2011-1390
更新日期:2011-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2010-1248
更新日期:2010-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-200887
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-2012-1531
更新日期:2012-01-01 00:00:00
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journal_title:Clinical hemorheology and microcirculation
pub_type: 杂志文章
doi:10.3233/CH-200817
更新日期:2020-01-01 00:00:00