当前位置： SCI文献检索 > CLINICAL HEMORHEOLOGY AND MICROCIRCULATION期刊下所有文献 > Subcellular localization of the chemotherapeutic agent doxorubicin in renal epithelial cells and in tumor cells using correlative light and electron microscopy.

Subcellular localization of the chemotherapeutic agent doxorubicin in renal epithelial cells and in tumor cells using correlative light and electron microscopy.

Abstract：

BACKGROUND:Doxorubicin is a cytostatic drug from the group of anthracycline antibiotics that is widely used as a chemotherapeutic agent. Side effects of the active substance include cardiotoxicity and nephrotoxicity. Doxorubicin-treated renal epithelial cells and (sarcoma) tumors are examined by correlative light and electron microscopy (CLEM) to investigate the subcellular localization of doxorubicin. METHODS:The kidney epithelial cell line MDCK II (Madin-Darby Canine Kidney) grown on culture dishes were treated with doxorubicin. Subsequently, the cells are analyzed by means of fluorescence and transmission electron microscopy (TEM). In vivo, alveolar rhabdomyosarcoma (RH 30) tumor cells are transferred to the chorioallantoic membrane (CAM) of the chicken embryo. Doxorubicin is injected into a vein of the chicken embryo. After 24 hours, the tumor is removed and examined using CLEM. RESULTS:The kidney epithelial cells and the doxorubicin-injected tumors show a clear staining of the cell nucleus, which correlates with electron-dense regions (heterochromatin). High-resolution TEM shows that doxorubicin treatment leads to an enormous stress situation with an increased formation of membrane blebbings. CONCLUSIONS:CLEM is a promising new method to visualize the pattern of fluorescing drugs (e.g. doxorubicin) in renal epithelial cells and tumors, and to localize the drug in its subcellular context combined with high resolution.

journal_name

Clin Hemorheol Microcirc

journal_title

Clinical hemorheology and microcirculation

authors

Asam C,Buerger K,Felthaus O,Brébant V,Rachel R,Prantl L,Witzgall R,Haerteis S,Aung T

doi

10.3233/CH-199212

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

157-167

issue

1

eissn

1386-0291

issn

1875-8622

pii

CH199212

journal_volume

73

pub_type

杂志文章

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