In vitro impact of physiological shear stress on endothelial cells gene expression profile.

Abstract:

:In the vascular system, the shear applied to the vascular wall activates mechano-sensors located on endothelial cells (ECs) leading to a modification in the gene expression profile. We applied laminar shear stress at 1 Pa on ECs for 6 h and measured by quantitative real time PCR the expression modulation of genes implied in inflammation (ICAM-1 and E-selectin), oxidative stress sensing (HO-1) and vascular tone modulation (eNOS). We showed that all these genes are shear stress inducible. ICAM-1 is more up-regulated than E-selectin suggesting different levels of implication in inflammatory responses and different modes of induction (SSRE, cytokine). Laminar shear stress induces an oxidative stress translated into HO-1 up-regulation, and a possible vasodilatation through the induction of eNOS. Our laminar shear stress system opens a novel and interesting frame in the evaluation of the impact on ECs and blood cells of new pharmacological substances injected in the bloodstream.

authors

Gaucher C,Devaux C,Boura C,Lacolley P,Stoltz JF,Menu P

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

99-107

issue

1-2

eissn

1386-0291

issn

1875-8622

journal_volume

37

pub_type

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