Abstract:
:The biological activity of 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3], 23(S)25(R)-1 alpha,25(OH)2D3-26,23-lactone, and three intermediate metabolites of the lactone in vivo and in vitro was comparatively examined. The three intermediate metabolites, 1 alpha,25(R)26(OH)3D3, 1 alpha,23(S)25(R)26(OH)4D3, and 23(S)25(R)-1 alpha,25(OH)2D3-26,23-lactol, stimulated increases, as did 1 alpha,25(OH)2D3, in intestinal calcium transport and serum calcium level in vitamin D-deficient rats fed a low-calcium diet. On the other hand, 23(S)25(R)-1 alpha,25(OH)2D3-26,23-lactone increased the calcium transport but decreased the serum calcium level. 1 alpha,25(OH)2D3,23(S)25(R)-Lactone and the other three metabolites stimulated multinucleate cell formation from hematopoietic blast cells in a manner correlated with their binding affinities for the 1 alpha,25(OH)2D3 receptor. But 23(S)25(R)-lactone did not show any inhibitory effect on the multinucleate cell formation induced by 1 alpha,25(OH)2D3 in contrast to the results obtained from unfractionated marrow cultures. Conditioned medium obtained from 23(S)25(R)-lactone-treated MC3T3-E1 cells inhibited the formation, probably by the action of some inhibitory factors elaborated by the cells treated with the lactone, whereas conditioned medium obtained from 1 alpha,25(OH)2D3 or other metabolite-treated MC3T3-E1 cells stimulated the formation. These findings suggest that 23(S)25(R)-1 alpha,25(OH)2D3-26,23-lactone might inhibit bone resorption through an inhibition of osteoclastic cell formation and that other vitamin D3 metabolites stimulate bone resorption by development of new osteoclastic cells in addition to indirect osteoclast activation.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Ishizuka S,Sumitani K,Hiura K,Kawata T,Okawa M,Hakeda Y,Kumegawa Mdoi
10.1210/endo-127-2-695subject
Has Abstractpub_date
1990-08-01 00:00:00pages
695-701issue
2eissn
0013-7227issn
1945-7170journal_volume
127pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Adult rats which have received monosodium-L-glutamate (MSG, 4 mg/g BW) on alternate days for the first 10 days of life manifest central nervous system lesions largely restricted to the retina and the arcuate nucleus of the hypothalamus in which nearly 90% of the perikarya are destroyed, leaving axons in passage intact...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-1-170
更新日期:1978-07-01 00:00:00
abstract::Type 2 deiodinase (D2), which is highly expressed in brown adipose tissue (BAT), is an enzyme that amplifies thyroid hormone signaling in individual cells. Mice with inactivation of the D2 pathway (D2KO) exhibit dramatically impaired thermogenesis in BAT, leading to hypothermia during cold exposure and a greater susce...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0511
更新日期:2010-09-01 00:00:00
abstract::The protein thiol-modifying agent arsenite, a potent activator of stress signaling, was used to examine the involvement of MAPKs in the regulation of cardiac substrate uptake. Arsenite strongly induced p38 MAPK phosphorylation in isolated rat cardiac myocytes but also moderately enhanced phosphorylation of p42/44 ERK ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0849
更新日期:2006-11-01 00:00:00
abstract::A study was conducted to determine the influence of conditions known to alter ACTH secretion on prolactin release by the pituitary. Dexamethasone or 0.9% NaCl was injected subcutaneously into intact male rats 4 hr before a 2-min ether stress. Other rats were submitted to the ether stress 2 weeks following adrenalectom...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-2-475
更新日期:1975-02-01 00:00:00
abstract::Thyroid hormones are essential for normal development and metabolism. Their synthesis requires transport of iodide into thyroid follicles. The mechanisms involving the apical efflux of iodide into the follicular lumen are poorly elucidated. The discovery of mutations in the SLC26A4 gene in patients with Pendred syndro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1548
更新日期:2012-01-01 00:00:00
abstract::Gonadotropin subunit gene expression is regulated by gonadal, hypothalamic, and locally derived hormones. In particular, activin rapidly (within hours) acts at the gonadotrope to selectively increase the expression of FSH beta messenger RNA (mRNA). A family of activin receptors (ActRI, ActRII, and ActRIIB) has been id...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.3.8070390
更新日期:1994-09-01 00:00:00
abstract::The thyroid hormone (TH) analog eprotirome (KB2115) was developed to lower cholesterol through selective activation of the TH receptor (TR) β1 in the liver. Interestingly, eprotirome shows low uptake in nonhepatic tissues, explaining its lipid-lowering action without adverse extrahepatic thyromimetic effects. Clinical...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00433
更新日期:2017-10-01 00:00:00
abstract::The alphaVbeta3 integrin is an important determinant of IGF-I-stimulated receptor phosphorylation and biological actions. Blocking ligand occupancy of alphaVbeta3 with the distintegrin echistatin reduces IGF-I-stimulated receptor phosphorylation, and it inhibits cellular migration and DNA synthesis responses to IGF-I....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220395
更新日期:2002-11-01 00:00:00
abstract::Pancreatic beta-cells and HIT-T15 cells exhibit oscillations of cytosolic calcium ([Ca2+]i) that are dependent on glucose metabolism and appear to trigger pulsatile insulin secretion. Significantly, differences in the pattern of this [Ca2+]i oscillatory activity may have important implications for our understanding of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013370
更新日期:1994-07-01 00:00:00
abstract::To analyze the roles of insulin receptor substrate (IRS) proteins in insulin-stimulated cell cycle progression, we examined the functions of rat IRS-1 and IRS-3 in Chinese hamster ovary cells overexpressing the human insulin receptor. In this type of cell overexpressing IRS-1 or IRS-3, we showed that: 1) overexpressio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0199
更新日期:2004-12-01 00:00:00
abstract::Ghrelin stimulates and leptin inhibits appetite by modulating neuropeptide Y (NPY) signaling in the hypothalamus. Analysis of plasma ghrelin and leptin by sensitive radioimmunoassays showed that the two peripheral hormones are secreted in pulsatile fashion in rats consuming ad libitum rat chow. Fasting augmented all p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.2.8743
更新日期:2002-02-01 00:00:00
abstract::The site(s) of the negative feedback action of estrogen on gonadotropin secretion were studied in the ovariectomized rhesus monkey by observing the serum LH and FSH responses to intravenous GnRH injections at various times after implantation of Silastic capsules filled with estrogen. Circulating estrogen concentration...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-5-1862
更新日期:1983-05-01 00:00:00
abstract::The present experiments investigated the time course of maternal modulation of GH secretion and examined the possible role of milk in the regulation of GH secretion in neonatal rats. Serum GH concentrations in neonatal rats were high at birth and declined over time postpartum. Separation of rat pups from their mothers...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1326
更新日期:1989-09-01 00:00:00
abstract::Diets lacking soy and alpha-alpha derivatives that are considered to be estrogen-free by standard bioassays (uterotrophic assay and vaginal opening) have been revealed to contain considerable amounts of compounds able to transcriptionally activate the estrogen receptors (ERs) and stimulate luciferase expression in sev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0660
更新日期:2005-12-01 00:00:00
abstract::Dynamic bone histomorphometry, [3H]thymidine radioautography, and Northern analysis for bone matrix proteins and insulin-like growth factor-I (IGF-I) were performed in calvariae of ovariectomized (OVX) and estrogen-treated OVX rats. Treatment of OVX rats with diethylstilbestrol (DES) for 2 weeks reduced the periosteal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.2.1639030
更新日期:1992-08-01 00:00:00
abstract::Uterine contractions at parturition depend upon a variety of factors, including gamma-aminobutyric acid (GABA)-ergic stimulation. A new subunit of the GABA(A) receptor, pi, has recently been identified as being particularly abundant in the rat uterus. Reduced derivatives of progesterone, such as the 3alpha,5alpha-redu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.5.8153
更新日期:2001-05-01 00:00:00
abstract::There are few data for hormonal levels and testis structure and function during postnatal development in rats neonatally treated with monosodium L-glutamate (MSG). In our study, newborn male pups were ip injected with MSG (4 mg/g body weight) every 2 d up to 10 d of age and investigated at prepubertal and adult ages. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0640
更新日期:2006-03-01 00:00:00
abstract::Aprotinin caused a dose-dependent inhibition of [125I]hCG binding to its receptor in plasma membranes prepared from luteinized rat ovaries. Displacement of [125I]hCG from its receptor by aprotinin was temperature dependent, with an ID50 of 300 microM at 4 C and an ID50 of 3700 microM at 22 C. Equilibrium binding data ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-3-946
更新日期:1987-03-01 00:00:00
abstract::The large-Maf transcription factor v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (MafA) has been found to be crucial for insulin transcription and synthesis and for pancreatic β-cell function and maturation. However, insights about the effects of small Maf factors on β-cells are limited. Our goal was to elu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1906
更新日期:2015-10-01 00:00:00
abstract::The mineralocorticoid receptor (MR) is a member of the steroid-thyroid hormone receptor superfamily of ligand-dependent transcription factors with diverse functions including the biological actions of aldosterone. Identification of the various transcriptional coregulators of MR is essential for understanding the compl...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00095
更新日期:2017-07-01 00:00:00
abstract::Disruption of the pituitary adenylate cyclase-activating polypeptide (PACAP) gene in mice has demonstrated a role for this highly conserved neuropeptide in the regulation of metabolism and temperature control. Localization of PACAP neurons within hypothalamic nuclei that regulate appetite suggest PACAP may affect feed...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0515
更新日期:2008-04-01 00:00:00
abstract::In the present study we have used a high affinity photoaffinity label (PAL) agonist (pGlu-His-Trp-Ser-125I-iodoTyr-D-Lys(para-N3-Benzoyl)-Leu-Arg-P roNHEt) and a PAL antagonist (NAcD2Nal-D4ClPhe-D3Pal-Ser-125I-iodo-NMeTyr-D-L ys(para-N3-Benzoyl)Leu-Lys(Isp) - Pro-D-Ala-NH2) to covalently label the GnRH receptor. Rat p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.8393780
更新日期:1993-08-01 00:00:00
abstract::In previous work we showed that the chondrodysplastic phenotype of mice homozygous for a null mutation of the PTH-related peptide (PTHrP) gene was due in part to reduced proliferation and aberrant differentiation of growth plate chondrocytes. In the present study we have extended those observations by examining chondr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895380
更新日期:1996-11-01 00:00:00
abstract::The importance of a central or peripheral site of action in the blockade of ovulation induced by LHRH antagonists was investigated. To determine if hCG could induce ovulation in cycling animals given antiovulatory doses of 10 or 100 micrograms [N-Ac-D-p-Cl-Phe1,D-p-Cl-Phe2,D-Trp3,D-Arg6,D-Ala10] LHRH, the antagonist w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-2-756
更新日期:1985-02-01 00:00:00
abstract::The establishment of dominant ovarian follicles that are capable of ovulating fertilizable oocytes is a fundamental determinant of female fertility. This process is governed by pituitary gonadotropins as well as local ovarian factors. Within the follicle, estrogen acts in an autocrine/paracrine manner to enhance FSH a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0349
更新日期:2005-08-01 00:00:00
abstract::IGF-I and IGF-II play important roles in growth and development via interactions with cell-surface receptors; however, in nature, IGFs are sequestered by at least six soluble, high-affinity IGF-binding proteins (IGFBPs), namely IGFBPs 1-6. Herein, we demonstrate that the stromal cell-derived extracellular matrix-degra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0636
更新日期:2004-02-01 00:00:00
abstract::Meiotically arrested mammalian oocytes are stimulated to resume meiosis by LH. This response, which can be reversed by elevation of intraoocyte cAMP levels, is associated with interruption of gap junctional communication (GJC) within the ovarian follicle. In the present study, we examined the hypothesis that disruptio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1011
更新日期:2006-05-01 00:00:00
abstract::To determine whether the insulinotropic effects of dopamine were mediated through the adrenergic system, dogs were infused with dopamine before or after various combinations of adrenergic and cholinergic blockade. Plasma glucose levels did not change significantly throughout the individual experiments, although cardio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-1-214
更新日期:1982-07-01 00:00:00
abstract::In the present study, we investigated the role of the multidrug resistance (mdr) P-glycoprotein (Pgp) at the blood-brain barrier in the control of access of cortisol and corticosterone to the mouse and human brain. [(3)H]Cortisol poorly penetrated the brain of adrenalectomized wild-type mice, but the uptake was 3.5-fo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.6.8213
更新日期:2001-06-01 00:00:00
abstract::Previous descriptions of pulsatile LH release in immature female rats have been mostly inaccurate because of infrequent blood sampling, discontinuous and unbalanced blood replacement procedures, and possibly because of the use of a heterologous RIA. The present study was undertaken to circumvent these problems and to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-2-644
更新日期:1985-08-01 00:00:00