Abstract：
:Trehalose synthase (TreS) catalyzes the reversible interconversion of maltose and trehalose. A novel treS gene with a length of 3,369 bp, encoding a protein of 1,122 amino acid residues with a predicted molecular mass of 126 kDa, was cloned from a marine Pseudomonas sp. P8005 (CCTCC: M2010298) and expressed in Escherichia coli. The amino acid sequence identities between this novel TreS and other reported TreS is relatively low. The purified recombinant TreS showed an optimum pH and temperature of 7.2 and 37 °C, respectively. The enzyme displayed a high conversion rate (70 %) of maltose to trehalose during equilibrium and had a higher catalytic efficiency (k cat/K m) for maltose than for trehalose, suggesting its application in the production of trehalose. In addition to maltose and trehalose, this enzyme can also act on sucrose, although this activity is relatively low. Mutagenesis studies demonstrated that enzymatic activity was reduced dramatically by individually substitution with alanine for D78, Y81, H121, D219, E261, H331 or D332, which implied that these residues might be important in P8005-TreS. Experiments using isotope-labeled substrates showed that [(2)H2]trehalose combined with unlabeled trehalose was converted to [(2)H2]maltose and maltose, but without any production of [(2)H]maltose or [(2)H]trehalose and with no incorporation of exogenous [(2)H7]glucose into the disaccharides during the conversion catalyzed by this enzyme. This finding indicated the involvement of an intramolecular mechanism in P8005-TreS catalyzing the reversible interconversion of maltose and trehalose.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Gao Y,Xi Y,Lu XL,Zheng H,Hu B,Liu XY,Jiao BHdoi
10.1007/s11274-013-1385-2subject
Has Abstractpub_date
2013-11-01 00:00:00pages
2195-206issue
11eissn
0959-3993issn
1573-0972journal_volume
29pub_type
杂志文章abstract：:Biotransformations have gained extensive importance in practical use as a support for chemical synthesis or in the conversion of natural products. Biotransformations may present an enlargement, a sequential degradation or a specific modification of synthetic or natural compounds. The tools for biotransformations are p...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF02421497
更新日期：1992-12-01 00:00:00
abstract：:With rapid urbanization, anthropogenic activities are increasingly influencing the natural environment of the Bohai Bay. In this study, the composition and variation of bacterial and nirS-harboring bacterial communities in the coastal zone sediments of the Bohai Gulf were analyzed using PCR-based clone libraries. A to...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1553-4
更新日期：2014-04-01 00:00:00
abstract：:The co-culture of cellulolytic moulds and yeasts on apple pomace in solid-state fermentation (SSF) and liquid-state fermentation (LSF) increased the protein content of apple pomace. The co-culture of Candida utilis and Aspergillus niger was the best among several combinations and increased the protein content of dried...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00357577
更新日期：1994-01-01 00:00:00
abstract：:In this study, we developed a novel technique for preparing polyvinyl alcohol (PVA) hydrogel as an immobilizing matrix by the addition of sodium bicarbonate. This resulted in an increase in the specific surface area of PVA_sodium bicarbonate (PVA_SB) hydrogel beads to 65.23 m(2) g(-1) hydrogel beads, which was approxi...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1621-4
更新日期：2014-07-01 00:00:00
abstract：:Biohydrogen is considered as one of the most promising energy alternatives considering the climate and energy crisis. The dark fermentative hydrogen production from xylose at extreme thermophilic condition (70 °C) using mixed culture was conducted in this study. The effects of initial pH values (ranged from 5.0 to 10....
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-016-2178-1
更新日期：2017-01-01 00:00:00
abstract：:Bacteria are crucial components in lake sediments and play important role in various environmental processes. Urban lakes in the densely populated cities are often small, shallow, highly artificial and hypereutrophic compared to rural and natural lakes and have been overlooked for a long time. In the present study, ba...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1126-y
更新日期：2012-11-01 00:00:00
abstract：:Eighteen non-sibling mutants of Pseudomonas aeruginosa PAO were isolated that were deficient in the utilization of the β-methyl branched acid citronellic acid but not in the utilization of the unbranched n-octanoic acid (Cau mutants). These mutants are also deficient in the utilization of citronellol and citronellal. ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00386294
更新日期：1993-09-01 00:00:00
abstract：:Anaerobic digestion (AD) is the most popular path of organic waste disposal. It is often used in wastewater treatment plants for excessive sludge removal. Methanogenic fermentation had usually been performed under mesophilic conditions, but in the past few years the thermophilic processes have become more popular due ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1731-z
更新日期：2014-12-01 00:00:00
abstract：:The search for new microbial strains that are able to withstand inhibitors released from hemicellulosic hydrolysis and are also still able to convert sugars in ethanol/xylitol is highly desirable. A yeast strain isolated from sugarcane juice and identified as Meyerozyma guilliermondii was evaluated for the ability to ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-016-2036-1
更新日期：2016-05-01 00:00:00
abstract：:The establishment of safe and effective methods for controlling fungal disease is an urgent issue in agriculture and forestry. Microbiological control of plant disease is expected to achieve better results than use of chemically derived fungicides. This study aimed to establish Brevibacillus laterosporus JX-5 as a pot...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-015-1912-4
更新日期：2015-10-01 00:00:00
abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) has become increasingly important because it is the most common cause of hospital-acquired infections, which have become globally epidemic. Our study specifically focused on the MRSA strain USA300, which was shown in 2014 to be responsible for the most current pandemi...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-015-1879-1
更新日期：2015-08-01 00:00:00
abstract：:Rice seedlings (Oryza sativa) inoculated with the plant growth-promoting rhizobacteria Azospirillum brasilense FT326 showed an enhanced development of the root system 3 days after inoculation. Later on, a remarkable enlargement of shoots was also evident. An increase in the Ca2+-dependent histone kinase activity was a...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-016-2186-1
更新日期：2017-02-01 00:00:00
abstract：:Saline wastewaters are usually generated by various industries, including the chemical, pharmaceutical, agricultural, and aquacultural industries. The discharge of untreated high-salinity wastewater may cause serious environmental pollution and damage the aquatic, terrestrial, and wetland ecosystems. For many countrie...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s11274-020-02815-4
更新日期：2020-02-22 00:00:00
abstract：:Andrimid, a known non-ribosomal pseudo-peptide antibiotic, was isolated from a psychrotolerant Serratia proteamaculans strain. The antibiotic peptide was produced at low temperature (8 °C) in a 7.5 l BIOFLO 101 bioreactor under batch culture mode. Andrimid activity from S. proteamaculans culture was only detected at 2...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1338-9
更新日期：2013-10-01 00:00:00
abstract：:A fungal isolate, Arthrographis sp. strain F4, when grown in shake-flask culture, produced cellulolytic and xylanolytic enzymes optimally at 30°C with an initial pH of 5.0 to 6.0. Coarsely-ground filter paper was the most suitable carbon substrate for production of the enzymes. Inorganic nitrogen sources gave higher a...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00383077
更新日期：1993-05-01 00:00:00
abstract：:Lactobacillus reuteri DPC16 is a probiotic bacterium that has strong antimicrobial activities on pathogens, mainly due to its ability to produce reuterin, an antimicrobial compound. The objective of this study was to examine the ability of an encapsulation technique to protect the functional properties of cells of L. ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0792-5
更新日期：2012-01-01 00:00:00
abstract：:Ginger (Zingiber officinale) is cultivated commercially in most parts of the world especially in India for its culinary and medicinal applications. One of the major challenges that limit the yield of ginger is rhizome rot disease caused by organisms including Pythium myriotylum. A feasible ecofriendly method is yet to...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1582-z
更新日期：2014-05-01 00:00:00
abstract：:Grain yields of the rice cultivar 'Prakash' were improved upon inoculation with Glomus intraradices and G. fasciculatum, by 11% and 8%, respectively, compared with an uninoculated control. The results indicate that the amount of phosphate fertilizer usually applied to rice may be decreased by 50%, without affecting yi...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00144455
更新日期：1994-07-01 00:00:00
abstract：:Terpenoids are a large class of compounds that have far-reaching applications and economic value, particularly those most commonly found in plants; however, the extraction and synthesis of these compounds is often expensive and technically challenging. Recent advances in microbial metabolic engineering comprise a brea...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-015-1975-2
更新日期：2016-01-01 00:00:00
abstract：:Over a 3-week period, samples of fresh, chopped, pork meat were taken every morning and afternoon from 50 meat stalls. Microbiological examination revealed that the samples had (c.f.u./g): total microbes, 1×10(3) to 2.14×10(6); mean probable numbers of coliforms and Escherichia coli, 1.51×10(3) to 1.15×10(4); and yeas...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00361025
更新日期：1995-11-01 00:00:00
abstract：:A mixed culture of a chlorobenzoate-(3-CBA)-degradingPseudomonas aeruginosa, strain 3mT, and a phenol/cresols-degradingPseudomonas sp., strain CP4, simultaneously and efficiently degraded mixtures of 3-CBA and phenol/cresols. However, strains 3mT and CP4 usedortho- andmeta-ring cleavage pathways, respectively. Degrada...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00704636
更新日期：1995-03-01 00:00:00
abstract：:The mushroom Flammulina velutipes and the white-rot fungus Trametes versicolor were cultivated separately on sugarcane bagasse for 40 days. Trametes versicolor produced laccase and manganese-peroxidase activities, showing a simultaneous degradation of lignin and holocellulose. However, only phenoloxidase activity was ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00286370
更新日期：1995-09-01 00:00:00
abstract：:A rapid, simple and sensitive method for detection of ferulic and p-coumaric acids using HPLC has been developed which can be used to determine the respective phenolic acid esterase activities of microorganisms. Prior concentration, purification or derivatization of the samples are not required. As little as 0.5 mg fe...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00360920
更新日期：1996-05-01 00:00:00
abstract：:The cloning and expression of toxic proteins in bacteria have posed a great challenge because of the leaky expression in inducible expression systems. Using artificial gene synthesis and clone screening methods, we identified a mutant T5 promoter, which significantly reduced leaky expression of lac operator. The mutan...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1573-0
更新日期：2014-05-01 00:00:00
abstract：:We describe a novel method for the real-time estimation of the accretion of blomass during the solid-substrate tempe fermentation of soy beans, lupins and quinoa by Rhizopus oligosporus Salto. The method is based on measurements of the dielectric permittivity at radio-frequencies, using a four-terminal instrument (the...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00328998
更新日期：1991-03-01 00:00:00
abstract：:The activity of sulfate reducing bacteria (SRB) in domestic wastewater treatment plants (WWTP) is often considered as a problem due to H2S formation and potential related odour and corrosion of materials. However, when controlled well, these bacteria can be effectively used in a positive manner for the treatment of wa...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s11274-015-1935-x
更新日期：2015-11-01 00:00:00
abstract：:Anaerobic degradation of organic matter follows similar pathways in digesters and anaerobic freshwater sediments. The responsible microorganisms are linked in a complex food web, where short chain fatty acids and H2 are important intermediates. Degradation of short-chain fatty acids is endothermic under standard condi...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00419463
更新日期：1996-09-01 00:00:00
abstract：:An extracellular polygalacturonase (EC 3.2.1.15) fromGeotrichum candidum ATCC 34614 grown onsauerkraut brine was produced and characterized. Polygalacturonic acid markedly increased the enzyme yield in the brine. The fungus produced the highest activity (290 U/l) in brine with 0.3% (w/v) polygalacturonic acid. The pH ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01201944
更新日期：1992-09-01 00:00:00
abstract：:Nikkomycin Z (NZ) is a competitive inhibitor of chitin synthase III in the yeast Saccharomyces cerevisiae. Myosin type II-deficient yeast strains (myo1) display a dramatic reduction in growth when chitin synthase III activity is inhibited by NZ, supporting the contention that actomyosin motility plays an important rol...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-005-9028-x
更新日期：2006-03-01 00:00:00
abstract：:The success of biotechnological research, development and marketing depends to a large extent on the international transfer of information and on the ability to organise biotechnology information into knowledge. To increase the efficiency of information-based approaches, an information strategy has been developed and ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00419453
更新日期：1996-09-01 00:00:00
