Production and characterization of polygalacturonase fromGeotrichum candidum.

Abstract:

:An extracellular polygalacturonase (EC 3.2.1.15) fromGeotrichum candidum ATCC 34614 grown onsauerkraut brine was produced and characterized. Polygalacturonic acid markedly increased the enzyme yield in the brine. The fungus produced the highest activity (290 U/l) in brine with 0.3% (w/v) polygalacturonic acid. The pH and temperature optima of the enzymes were 4.5 to 5.0 and 30°C, respectively. It was stable from pH 4.0 to 5.8 and at 30°C but lost its activity at higher temperatures. The Km and Vmax values for polygalacturonic acid were 4.2 mg/ml and 0.19MM galacturonic acid/min, respectively. The enzyme was not substrate inhibited.

authors

Hang YD,Woodams EE

doi

10.1007/BF01201944

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

480-2

issue

5

eissn

0959-3993

issn

1573-0972

journal_volume

8

pub_type

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