Abstract：
:Drotaverine [1-(3,4-diethoxybenzylidene)-6,7-diethoxy-1,2,3,4-tetrahydroisoquinoline] hydrochloride, an antispasmodic drug derived from benzylisoquinoline was evaluated for its biodegradability using a bacterial strain Rhodococcus rhodochrous IEGM 608. The experiments were performed under aerobic conditions with rhodococci cultures able to degrade drotaverine. In the presence of glucose, the removal efficiency of drotaverine by free Rhodoccocus cells pre-grown with isoquinoline was above 80 % (200 mg/l, initial concentration) after 25 days. Rhodococcus immobilization on hydrophobized sawdust enhanced the biodegradation process, with the most marked drotaverine loss being observed during the first 5 days of fermentation. High metabolic activity of rhodococcal cells towards drotaverine was confirmed respirometrically. GC-MS analysis of transformation products resulting from drotaverine biodegradation revealed 3,4-diethoxybenzoic acid, 3,4-diethoxybenzaldehyde and 3,4-diethoxybenzoic acid ethyl ester which were detected in the culture medium until drotaverine completely disappeared. Based on these major and other minor metabolites, putative pathways for drotaverine biodegradation were proposed. The obtained data broadened the spectrum of organic xenobiotics oxidized by Rhodoccocus bacteria and proved their potential in decontamination of natural ecosystems from pharma pollutants.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Ivshina IB,Vikhareva EV,Richkova MI,Mukhutdinova AN,Karpenko JNdoi
10.1007/s11274-012-1110-6subject
Has Abstractpub_date
2012-10-01 00:00:00pages
2997-3006issue
10eissn
0959-3993issn
1573-0972journal_volume
28pub_type
杂志文章abstract：:The methylotrophic yeast Pichia pastoris is widely used in recombinant expression of eukaryotic proteins owing to the ability of post-translational modification, tightly regulated promoters, and high cell density fermentation. However, episomal plasmids for heterologous gene expression and the CRISPR/Cas9 system for g...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-019-2654-5
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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doi：10.1007/BF00419453
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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更新日期：2012-04-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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doi：10.1007/s11274-014-1645-9
更新日期：2014-08-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-019-2653-6
更新日期：2019-05-27 00:00:00
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journal_title：World journal of microbiology & biotechnology
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更新日期：2015-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-017-2399-y
更新日期：2018-02-15 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1724-y
更新日期：2014-11-01 00:00:00
abstract：:The biomass degrading enzymatic potential of 101 thermophilic bacterial strains isolated from a volcanic environment (Santorini, Aegean Sea, Greece) was assessed. 80 % of the strains showed xylanolytic activity in Congo Red plates, while only eight could simultaneously hydrolyze cellulose. Fifteen isolates were select...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1100-8
更新日期：2012-09-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1584-x
更新日期：2014-05-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01201871
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-018-2440-9
更新日期：2018-03-28 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2016-08-01 00:00:00
abstract：:Common reed (Phragmites australis) and narrow-leaved cattail (Typha angustifolia L.) are two plant species used widely in artificial wetlands constructed to treat wastewater. In this study, the community structure and diversity of root-associated bacteria of common reed and narrow-leaved cattail growing in the Beijing...
journal_title：World journal of microbiology & biotechnology
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doi：10.1007/s11274-013-1316-2
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01198762
更新日期：1992-07-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1656-6
更新日期：2014-08-01 00:00:00
abstract：:Soaking seeds of cucumber and pumpkin with an extract of Westiellopsis prolifica, an N2-fixing cyanobacterium, promoted germination and their subsequent growth and development. An extract of Lyngbya sp., a non-N2-fixing cyanobacterium, had no significant effect. ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00452845
更新日期：1991-11-01 00:00:00
abstract：:Diarrhoea due toVibrio cholerae non-01 is common in Bangladesh. Four hundred and eighty samples, including plants, water, phytoplankton and sediment, were collected from five ponds in Dhaka every 15 days for one year.V. cholerae non-01 was isolated from 181 (38%) of the samples. Two peaks were evident: one in April an...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：1992-03-01 00:00:00
abstract：:Silver nanoparticles (SNPs) are extensively used in many industrial and medical applications; however, the impact of their release in the environment is still considered an understudied field. In the present work, SNPs present in aqueous lab waste water (average size of 30 nm) were used to determine their impact on mi...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1597-0
更新日期：2014-06-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0813-4
更新日期：2012-01-01 00:00:00
abstract：:The bioleachings of chalcopyrite ore were compared after inoculating different cultures enriched from the original acid mine drainage sample. The results showed that the higher bioleaching performance was achieved for inoculation with the enrichment D (0.5 % S, 2 % iron and 1 % chalcopyrite) compared to other enrichme...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1179-y
更新日期：2013-02-01 00:00:00
