Abstract:
:The biomass degrading enzymatic potential of 101 thermophilic bacterial strains isolated from a volcanic environment (Santorini, Aegean Sea, Greece) was assessed. 80 % of the strains showed xylanolytic activity in Congo Red plates, while only eight could simultaneously hydrolyze cellulose. Fifteen isolates were selected on the basis of their increased enzyme production, the majority of which was identified as Geobacilli through 16S rDNA analysis. In addition, the enzymatic profile was evaluated in liquid cultures using various carbon sources, a procedure that revealed lack of correlation on xylanase levels between the two cultivation modes and the inability of solid CMC cultures to fully unravel the cellulose degrading potential of the isolates. Strain SP24, showing more than 99 % 16S DNA similarity with Geobacillus sp. was further studied for its unique ability to simultaneously exhibit cellulase, xylanase, β-glucosidase and β-xylosidase activities. The first two enzymes were produced mainly extracellularly, while the β-glycosidic activities were primarily detected in the cytosol. Maximum enzyme production by this strain was attained using a combination of wheat bran and xylan in the growth medium. Bioreactor cultures showed that aeration was necessary for both enhanced growth and enzyme production. Aeration had a strong positive effect on cellulase production while it negatively affected expression of β-glucosidase. Xylanase and β-xylosidase production was practically unaffected by aeration levels.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Stathopoulou PM,Galanopoulou AP,Anasontzis GE,Karagouni AD,Hatzinikolaou DGdoi
10.1007/s11274-012-1100-8subject
Has Abstractpub_date
2012-09-01 00:00:00pages
2889-902issue
9eissn
0959-3993issn
1573-0972journal_volume
28pub_type
杂志文章abstract::To simplify industrial mushroom cultivation, we introduced a bacterial Pseudomonas sp. UW4 acdS gene, encoding 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (AcdS), into fungus Agaricus bisporus. Transformant A. bisporus-acdS14 cased with sterilized-vermiculite generated primordia 5 days sooner than wild-type ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2741-7
更新日期:2019-10-21 00:00:00
abstract::Wetland degradation makes significant impacts on soil, and bacterial communities in soil are likely to respond to these impacts. The purpose of this study was to investigate the impacts of soil property, soil type and soil depth on bacterial community in different stages of soil degradation in the Zoige Wetland. Micro...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-011-0858-4
更新日期:2012-02-01 00:00:00
abstract::Progesterone was completely hydroxylated, principally to 11β,14α dihydroxyprogesterone by Cunninghamella blakesleeana NCIM 687 in 48h. ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00367121
更新日期:1995-05-01 00:00:00
abstract::White rot fungi are well known for their ability to degrade xenobiotics in pure cultures but few studies focus on their performance under bacterial stress in real wastewaters. This study investigated mutual interactions in co-cultures of Pleurotus ostreatus and activated sludge microbes in batch reactors and different...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-016-2050-3
更新日期:2016-06-01 00:00:00
abstract::The potential of three Serratia marcescens strains (CFFSUR-B2, CFFSUR-B3 and CFFSUR-B4) isolated from tropical regions in Mexico to inhibit the mycelial growth and conidial germination of Colletotrichum gloeosporioides, causal agent of fruit anthracnose, was evaluated. The ability of these strains to produce prodigios...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-011-0803-6
更新日期:2012-01-01 00:00:00
abstract::The cloning and expression of toxic proteins in bacteria have posed a great challenge because of the leaky expression in inducible expression systems. Using artificial gene synthesis and clone screening methods, we identified a mutant T5 promoter, which significantly reduced leaky expression of lac operator. The mutan...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1573-0
更新日期:2014-05-01 00:00:00
abstract::Microbial electrochemical system (MES) has attracted ever-growing interest as a promising platform for renewable energy conversion and bioelectrochemical remediation. Shewanella species, the dissimilatory metal reduction model bacteria with versatile extracellular electron transfer (EET) strategies, are the well-recei...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s11274-018-2576-7
更新日期:2018-12-19 00:00:00
abstract::Polyhydroxyalkanoates (PHAs) are a class of high-molecular-weight polyesters made from hydroxy fatty acid monomers. PHAs produced by microorganisms have diverse structures, variable physical properties, and good biodegradability. They exhibit similar physical properties to petroleum-based plastics but are much more en...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s11274-020-02986-0
更新日期:2021-01-04 00:00:00
abstract::Rice bran was superior to other proteinaceous substrates for protease production by Rhizopus oligosporus ACM 145F in solid-state fermentation. Maximum protease yield was after 72 h. The optimal initial moisture content was 47% (a w=0.97). Dried, ground and resuspended fermented rice was the most pratical and effective...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00414872
更新日期:1994-05-01 00:00:00
abstract::Acetic acid is an inhibitor in industrial processes such as wine making and bioethanol production from cellulosic hydrolysate. It causes energy depletion, inhibition of metabolic enzyme activity, growth arrest and ethanol productivity losses in Saccharomyces cerevisiae. Therefore, understanding the mechanisms of the y...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s11274-017-2259-9
更新日期:2017-05-01 00:00:00
abstract::Filamentous fungi, isolated from the enriched surface of garden soil, were screened for α-galactosidase production after growing on wheat-bran/carbohydrate substrate. One isolate,Aspergillus niger, had the highest enzyme activity (5.1×10(-2) units/mg protein) at pH 5.0 and 50°C. Treatment of cowpea flour with the crud...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF01238789
更新日期:1992-11-01 00:00:00
abstract::This paper aims to characterize halophilic bacteria inhabiting Algerian Saline Ecosystems (Sebkha and Chott) located in arid and semi-arid ecoclimate zones (Northeastern Algeria). In addition, screening of enzymatic activities, heavy metal tolerance and antagonistic potential against phytopathogenic fungi were tested....
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2705-y
更新日期:2019-08-20 00:00:00
abstract::Purified cell walls from Bacillus subtilis were repeatedly suspended in 5 mM CuCl2 and, after removing unbound Cu, were suspended in 1% (v/v) HNO3 to release bound Cu. The walls were then regenerated by washing in H2O. After five cycles, copper binding actually increased slightly, probably due to enhanced exposure of ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00144475
更新日期:1994-07-01 00:00:00
abstract::In the present work, novel heterotrophic nitrifying and aerobic denitrifying bacteria have been isolated from greenwater system of coastal aquaculture. Based on the 16S rRNA gene, FAME analysis and biochemical test, the isolates have been identified as Pseudomonas aeruginosa and Achromobacter sp. These have been named...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1543-6
更新日期:2014-04-01 00:00:00
abstract::Shikimic acid (SA) is the key synthetic material of Oseltamivir, which is an effective drug for the prevention and treatment of influenza. In this study, to block the downstream metabolic pathway of SA, the shikimate kinase isoenzyme genes aroK and aroL were deleted by Red recombination. Moreover, the key enzyme genes...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1679-z
更新日期:2014-09-01 00:00:00
abstract::Species of the genus Trichoderma are economically important as biocontrol agents, serving as a potential alternative to chemical control. The applicability of Trichoderma isolates to different ecozones will depend on the behavior of the strains selected from each zone. The present study was undertaken to isolate bioco...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-015-1989-9
更新日期:2016-03-01 00:00:00
abstract::The methylotrophic yeast Pichia pastoris is widely used in recombinant expression of eukaryotic proteins owing to the ability of post-translational modification, tightly regulated promoters, and high cell density fermentation. However, episomal plasmids for heterologous gene expression and the CRISPR/Cas9 system for g...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2654-5
更新日期:2019-05-27 00:00:00
abstract::Aquilaria malaccensis produces agarwood in response to wounding and fungal attack. However, information is limited regarding Aquilaria's interaction with its diverse fungal community. In this study, time-related changes of three natural fungal colonizers in two wounded wild A. malaccensis were tracked, beginning a few...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1668-2
更新日期:2014-09-01 00:00:00
abstract::In a search for microorganisms producing extracellular protease with unhairing activity, Bacillus subtilis IIQDB32 was isolated. Protease formation was significantly stimulated by glucose, tryptone, yeast extract, Ca(2+) and Mn(2+), but was repressed by ammonia and Fe(2+). ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00327730
更新日期:1996-11-01 00:00:00
abstract::Butanol is not only an important chemical intermediate and solvent in pharmaceutical and cosmetics industries, but also considered as an advanced biofuel. Although species of the natural host Clostridium have been engineered, butanol titers in the anaerobe seem to be limited by its intolerance to butanol less than 13 ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-017-2246-1
更新日期:2017-04-01 00:00:00
abstract::agWH50C, a novel β-agarase gene, was cloned from Agarivorans gilvus WH0801 by degenerate PCR and nested PCR. The gene agWH50C comprized a 2,223-bp, encoding a protein of 740 amino acids. Sequencing results demonstrated that AgWH50C shared 45 % sequence identity with a well characterized β-agarase, Aga50D, from Sacchar...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1591-y
更新日期:2014-06-01 00:00:00
abstract::Brucellae are Gram-negative intracellular bacterial pathogens that infect humans and animals, bringing great economic burdens to developing countries. Live attenuated Brucella vaccines (strain M5-90 or others) are the most efficient means for prevention and control of animal brucellosis. However, these vaccines have s...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-016-2015-6
更新日期:2016-04-01 00:00:00
abstract::Biotransformations have gained extensive importance in practical use as a support for chemical synthesis or in the conversion of natural products. Biotransformations may present an enlargement, a sequential degradation or a specific modification of synthetic or natural compounds. The tools for biotransformations are p...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF02421497
更新日期:1992-12-01 00:00:00
abstract::Ersho is a clear, yellow liquid that accumulates on the surface of fermenting teff-flour batter and is collected to serve as an inoculum for the next fermentation. The pH of ersho samples was about 3.5 and titratable acidity ranged between 3.1% and 5.7%. The mean aerobic mesophilic counts from four households varied b...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00357567
更新日期:1994-01-01 00:00:00
abstract::The mushroom Flammulina velutipes and the white-rot fungus Trametes versicolor were cultivated separately on sugarcane bagasse for 40 days. Trametes versicolor produced laccase and manganese-peroxidase activities, showing a simultaneous degradation of lignin and holocellulose. However, only phenoloxidase activity was ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00286370
更新日期:1995-09-01 00:00:00
abstract::An analytical procedure has been developed for at-line (fast off-line) monitoring of 4 key parameters including nisin titer (NT), the concentration of reducing sugars, cell concentration and pH during a nisin fermentation process. This procedure is based on near infrared (NIR) spectroscopy and Partial Least Squares (P...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-011-0897-x
更新日期:2012-03-01 00:00:00
abstract::Bacillus coagulans, when grown on casein at 20°C, produced an inducible, metalloprotease of 28 kDa at 1.6 U/mg cell protein. (NH4)2SO4 at 2 g/l decreased enzyme production irrespective of carbon source. ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00414882
更新日期:1994-05-01 00:00:00
abstract::Psychrophiles are organisms that thrive in cold environments. One of the strategies for their cold adaptation is the ability to synthesize cold-adapted enzymes. These enzymes usually display higher catalytic efficiency and thermolability at lower temperatures compared to their mesophilic and thermophilic counterparts....
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-009-0295-9
更新日期:2010-07-01 00:00:00
abstract:CONCLUSION:The basis for science and technology in advanced nations might be considered to have come from Egypt, Babylonia, China or ancient countries. Thus, technologies belong to all peoples in the world and should be shared with all countries. The traditional brewing of sake is a truly indigenous and ancient process...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF02421507
更新日期:1992-12-01 00:00:00
abstract::Fossil fuels consumption impacts the greenhouse gas emissions. Biofuels are considered as alternative renewable energy sources to reduce the fossil fuels dependency. Bioethanol produced by recombinant microorganisms is a widely suggested alternative to increase the yield in fermentation processes. However, ethanol and...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2769-8
更新日期:2019-11-20 00:00:00