Abstract：
:agWH50C, a novel β-agarase gene, was cloned from Agarivorans gilvus WH0801 by degenerate PCR and nested PCR. The gene agWH50C comprized a 2,223-bp, encoding a protein of 740 amino acids. Sequencing results demonstrated that AgWH50C shared 45 % sequence identity with a well characterized β-agarase, Aga50D, from Saccharophagus degradans 2-40. The mature agarase was expressed in Escherichia coli and purified by affinity chromatography. The optimum pH and temperature for AgWH50C activity were 6.0 and 30 °C. The K m and V max values for agarose were 12.55 mg/ml and 1.17 U/mg. Analysis of the hydrolysis products using linear ion trap mass spectrometry, Fourier transform-nuclear magnetic resonance spectrometry and thin-layer chromatography confirmed that the reaction product of AgWH50c was α-neoagarobiose alone. Therefore, our novel agarase has the potential for industrial applications to produce neoagarobiose as well as provides a key β-agarase for fermentation of agar biomass.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Liu N,Mao X,Yang M,Mu B,Wei Ddoi
10.1007/s11274-013-1591-ysubject
Has Abstractpub_date
2014-06-01 00:00:00pages
1691-8issue
6eissn
0959-3993issn
1573-0972journal_volume
30pub_type
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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更新日期：2019-08-28 00:00:00
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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