Abstract:
:The cloning and expression of toxic proteins in bacteria have posed a great challenge because of the leaky expression in inducible expression systems. Using artificial gene synthesis and clone screening methods, we identified a mutant T5 promoter, which significantly reduced leaky expression of lac operator. The mutant T5 promoter contains two T deletions at -35 region and may reduce promoter activity. A bacterial lethal gene, Φ174 lytic gene E, was successfully cloned in this system and expressed in the presence of isopropyl β-D-1-thiogalactopyranoside. The system is compatible with existing T5 inducible expression systems and can be used for the controlled expression of toxic proteins.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Guo J,Jia Rdoi
10.1007/s11274-013-1573-0subject
Has Abstractpub_date
2014-05-01 00:00:00pages
1527-31issue
5eissn
0959-3993issn
1573-0972journal_volume
30pub_type
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journal_title:World journal of microbiology & biotechnology
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journal_title:World journal of microbiology & biotechnology
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journal_title:World journal of microbiology & biotechnology
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