A novel inducible expression system for the functional study of toxic gene in bacteria.

Abstract:

:The cloning and expression of toxic proteins in bacteria have posed a great challenge because of the leaky expression in inducible expression systems. Using artificial gene synthesis and clone screening methods, we identified a mutant T5 promoter, which significantly reduced leaky expression of lac operator. The mutant T5 promoter contains two T deletions at -35 region and may reduce promoter activity. A bacterial lethal gene, Φ174 lytic gene E, was successfully cloned in this system and expressed in the presence of isopropyl β-D-1-thiogalactopyranoside. The system is compatible with existing T5 inducible expression systems and can be used for the controlled expression of toxic proteins.

authors

Guo J,Jia R

doi

10.1007/s11274-013-1573-0

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

1527-31

issue

5

eissn

0959-3993

issn

1573-0972

journal_volume

30

pub_type

杂志文章
  • Biological activity of quaternary ammonium salts and resistance of microorganisms to these compounds.

    abstract::Quaternary ammonium salts (QASs) are ubiquitous in nature, being found in organisms ranging from microorganisms to vertebrates (e.g., glycine betaine, carnitine) where they have important cellular functions. QASs are also obtained by chemical synthesis. These compounds, due to their diverse chemical structure (e.g. mo...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s11274-020-02978-0

    authors: Obłąk E,Futoma-Kołoch B,Wieczyńska A

    更新日期:2021-01-11 00:00:00

  • Aspects for the future of biotransformations.

    abstract::Biotransformations have gained extensive importance in practical use as a support for chemical synthesis or in the conversion of natural products. Biotransformations may present an enlargement, a sequential degradation or a specific modification of synthetic or natural compounds. The tools for biotransformations are p...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02421497

    authors: Kieslich K

    更新日期:1992-12-01 00:00:00

  • Hydroxylation of progesterone by Cunninghamella blakesleeana NCIM 687.

    abstract::Progesterone was completely hydroxylated, principally to 11β,14α dihydroxyprogesterone by Cunninghamella blakesleeana NCIM 687 in 48h. ...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00367121

    authors: Chincholkar SB,Seeta Laxman R,Wakharkar RD

    更新日期:1995-05-01 00:00:00

  • Protein nutritional value, polyphenols and antioxidant properties of corn fermented with Agaricus brasiliensis and Agaricus bisporus.

    abstract::Solid-state fermentation (SSF) with Agaricus brasiliensis and Agaricus bisporus on corn was carried out. The results showed that SSF with the two fungi made up the deficiency of tryptophan in corn and improved the protein nutritional value of corn. The conjugated polyphenols contents in fermented corn decreased and fr...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-017-2399-y

    authors: Zhai FH,Liu HY,Han JR

    更新日期:2018-02-15 00:00:00

  • Three polygalacturonases constitutively synthesized by Aspergillus alliaceus.

    abstract::Of three molecular forms of polygalacturonases synthesized by Aspergillus alliaceus on glucose media, two were exopolygalacturonases (exoPG1 and exoPG2) and one was an endopolygalacturonase (endoPG). Low-methoxylated beet pectin was the preferred substrate for the endoPG and exoPG2 whereas pectic acid was the optimal ...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00367111

    authors: Mikhailova RV,Sapunova LI,Lobanok AG

    更新日期:1995-05-01 00:00:00

  • Comparison of chalcopyrite bioleaching after different microbial enrichment in shake flasks.

    abstract::The bioleachings of chalcopyrite ore were compared after inoculating different cultures enriched from the original acid mine drainage sample. The results showed that the higher bioleaching performance was achieved for inoculation with the enrichment D (0.5 % S, 2 % iron and 1 % chalcopyrite) compared to other enrichme...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-012-1179-y

    authors: Xia L,Uribe P,Liu X,Yu C,Chai L,Liu J,Qiu W,Qiu G

    更新日期:2013-02-01 00:00:00

  • In vitro plant development and root colonization of Coleus forskohlii by Piriformospora indica.

    abstract::The present study was conducted for optimization of in vitro substrates under aseptic conditions for interaction of Piriformospora indica with the medicinal plant Coleus forskohlii. It aims to test the effects of different substrates on P. indica colonization as well as growth parameters of the in vitro raised C. fors...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-013-1526-7

    authors: Das A,Tripathi S,Varma A

    更新日期:2014-03-01 00:00:00

  • Selection of rhizosphere-competent Pseudomonas strains as biocontrol agents in tropical soils.

    abstract::Pseudomonas strains were isolated from the rhizosphere of maize grown in yellow-red latosol from Rio de Janeiro, Brazil, to serve as a delivery system for heterologous genes and for risk assessment studies in tropical soils. Selected strains were modified by insertion of the cryIVB gene from Bacillus thuringiensis and...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00327720

    authors: Araújo MA,Mendonça-Hagler LC,Hagler AN,van Elsas JD

    更新日期:1996-11-01 00:00:00

  • A simple method for isolation of high-molecular-weight DNA from Rhodotorula gracilis.

    abstract::Unsheared DNA has been isolated from Rhodotorula and Rhodosporidium yeasts using a cell-wall-digesting enzyme preparation from Paecilomyces lilacinus. Pulsed-field gel electrophoresis indicated that at least 11 chromosomes were present in Rhodot. gracilis ATCC 90950. The DNA was amenable to digestion with restriction ...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00367101

    authors: Vishnuvardhan D,Joseph R

    更新日期:1995-05-01 00:00:00

  • Azospirillum spp. from native forage grasses in Brazilian Pantanal floodplain: biodiversity and plant growth promotion potential.

    abstract::A sustainable alternative to improve yield and the nutritive value of forage is the use of plant growth-promoting bacteria (PGPB) that release nutrients, synthesize plant hormones and protect against phytopathogens (among other mechanisms). Azospirillum genus is considered an important PGPB, due to the beneficial effe...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-017-2251-4

    authors: Souza MS,de Baura VA,Santos SA,Fernandes-Júnior PI,Reis Junior FB,Marques MR,Paggi GM,da Silva Brasil M

    更新日期:2017-04-01 00:00:00

  • Pseudomonas aeruginosa and Achromobacter sp.: nitrifying aerobic denitrifiers have a plasmid encoding for denitrifying functional genes.

    abstract::In the present work, novel heterotrophic nitrifying and aerobic denitrifying bacteria have been isolated from greenwater system of coastal aquaculture. Based on the 16S rRNA gene, FAME analysis and biochemical test, the isolates have been identified as Pseudomonas aeruginosa and Achromobacter sp. These have been named...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-013-1543-6

    authors: Kathiravan V,Krishnani KK

    更新日期:2014-04-01 00:00:00

  • Activation of a calcium-dependent protein kinase involved in the Azospirillum growth promotion in rice.

    abstract::Rice seedlings (Oryza sativa) inoculated with the plant growth-promoting rhizobacteria Azospirillum brasilense FT326 showed an enhanced development of the root system 3 days after inoculation. Later on, a remarkable enlargement of shoots was also evident. An increase in the Ca2+-dependent histone kinase activity was a...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-016-2186-1

    authors: Ribaudo CM,Curá JA,Cantore ML

    更新日期:2017-02-01 00:00:00

  • Antifungal activity of Brevibacillus laterosporus JX-5 and characterization of its antifungal components.

    abstract::The establishment of safe and effective methods for controlling fungal disease is an urgent issue in agriculture and forestry. Microbiological control of plant disease is expected to achieve better results than use of chemically derived fungicides. This study aimed to establish Brevibacillus laterosporus JX-5 as a pot...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-015-1912-4

    authors: Jiang H,Wang X,Xiao C,Wang W,Zhao X,Sui J,Sa R,Guo TL,Liu X

    更新日期:2015-10-01 00:00:00

  • The effect of immobilization of probiotic Lactobacillus reuteri DPC16 in sub-100 μm microcapsule on food-borne pathogens.

    abstract::The aim of this study was to evaluate whether immobilizing a probiotic strain Lactobacillus reuteri DPC16 in chitosan-coated alginate microcapsules affected their inhibitory performance against food-borne pathogens. The probiotic strain was encapsulated in sub-100 μm alginate microspheres which were further coated wit...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-012-1046-x

    authors: Chen S,Cao Y,Ferguson LR,Shu Q,Garg S

    更新日期:2012-06-01 00:00:00

  • Isolation, diversity and acetylcholinesterase inhibitory activity of the culturable endophytic fungi harboured in Huperzia serrata from Jinggang Mountain, China.

    abstract::Huperzia serrata has many important medicinal properties with proven pharmacological potential. Some of these properties may be mediated by its endophytic fungi. To test this hypothesis, in the present study, we provided a first insights into evaluating the species composition and acetylcholinesterase (AChE) inhibitor...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-015-1966-3

    authors: Wang Y,Lai Z,Li XX,Yan RM,Zhang ZB,Yang HL,Zhu D

    更新日期:2016-02-01 00:00:00

  • Lichen secondary metabolite evernic acid as potential quorum sensing inhibitor against Pseudomonas aeruginosa.

    abstract::Cystic Fibrosis is a genetic disease and it affects the respiratory and digestive systems. Pseudomonas aeruginosa infections in Cystic Fibrosis are presented as the main cause for high mortality and morbidity rates. Pseudomonas aeruginosa populations can regulate their virulence gene expressions via the bacterial comm...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-016-2105-5

    authors: Gökalsın B,Sesal NC

    更新日期:2016-09-01 00:00:00

  • Cloning, expression and functional characterization of a novel trehalose synthase from marine Pseudomonas sp. P8005.

    abstract::Trehalose synthase (TreS) catalyzes the reversible interconversion of maltose and trehalose. A novel treS gene with a length of 3,369 bp, encoding a protein of 1,122 amino acid residues with a predicted molecular mass of 126 kDa, was cloned from a marine Pseudomonas sp. P8005 (CCTCC: M2010298) and expressed in Escheri...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-013-1385-2

    authors: Gao Y,Xi Y,Lu XL,Zheng H,Hu B,Liu XY,Jiao BH

    更新日期:2013-11-01 00:00:00

  • Two new 17 α-hydroxyprogesterone transformation products fromNocardia DSM 43298.

    abstract::Two new products: 9,10-seco-1,3,5(10)-pregnatrien-3,17 a-dihydroxy-9,20-dione and 9,10-seco-1,3,5(10)-pregnatrien-3,17a,20-triol-9-one, have been isolated as 17 α-hydroxyprogesterone transformation products fromNocardia DSM 43298. The structures were established by spectroscopic methods. ...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00656517

    authors: Choudhry S,Halos S,Krischenowski D,Schmeda-Hirschmann G

    更新日期:1993-01-01 00:00:00

  • Growth enhancing effect of exogenous glycine and characterization of its uptake in halotolerant cyanobacterium Aphanothece halophytica.

    abstract::Alkaliphilic halotolerant cyanobacterium Aphanothece halophytica showed optimal growth in the medium containing 0.5 M NaCl. The increase of exogenously added glycine to the medium up to 10 mM significantly promoted cell growth under both normal (0.5 M NaCl) and salt stress (2.0 M NaCl) conditions. Salt stress imposed ...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-014-1790-1

    authors: Bualuang A,Incharoensakdi A

    更新日期:2015-02-01 00:00:00

  • Functional properties of free and encapsulated Lactobacillus reuteri DPC16 during and after passage through a simulated gastrointestinal tract.

    abstract::Lactobacillus reuteri DPC16 is a probiotic bacterium that has strong antimicrobial activities on pathogens, mainly due to its ability to produce reuterin, an antimicrobial compound. The objective of this study was to examine the ability of an encapsulation technique to protect the functional properties of cells of L. ...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-011-0792-5

    authors: Zhao Q,Mutukumira A,Lee SJ,Maddox I,Shu Q

    更新日期:2012-01-01 00:00:00

  • Talaromyces australis and Penicillium murcianum pigment production in optimized liquid cultures and evaluation of their cytotoxicity in textile applications.

    abstract::In this work Talaromyces australis and Penicillium murcianum pigment production in liquid cultures and the cytotoxic effect of such pigments on skin model cells were studied. Response surface methodology (RSM) was used to optimize culture conditions aiming to increase pigment production in malt extract and peptone-glu...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-019-2738-2

    authors: Hernández VA,Machuca Á,Saavedra I,Chavez D,Astuya A,Barriga C

    更新日期:2019-10-12 00:00:00

  • Production of xylanases by mangrove fungi from the Philippines and their application in enzymatic pretreatment of recycled paper pulps.

    abstract::Mangrove fungi are vastly unexplored for enzymes with industrial application. This study aimed to assess the biocatalytic activity of mangrove fungal xylanases on recycled paper pulp. Forty-four mangrove fungal (MF) isolates were initially screened for xylanolytic activity in minimal medium with corn cob xylan as the ...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-012-1220-1

    authors: Torres JM,Dela Cruz TE

    更新日期:2013-04-01 00:00:00

  • Microbial community and physicochemical dynamics during the production of 'Chicha', a traditional beverage of Indigenous people of Brazil.

    abstract::The microbial community of artisanal corn fermentation called Chicha were isolated, purified and then identified using protein profile by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) and confirmed by partial ribosomal gene sequencing. Samples from Chicha beverage were chemic...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-018-2429-4

    authors: Resende LV,Pinheiro LK,Miguel MGDCP,Ramos CL,Vilela DM,Schwan RF

    更新日期:2018-03-08 00:00:00

  • Diversity and antimicrobial activity of culturable fungi from fishscale bamboo (Phyllostachys heteroclada) in China.

    abstract::An important and useful bamboo species, fishscale bamboo (Phyllostachys heteroclada Oliver), is broadly distributed in Southeast China and has multiple purposes, including uses in cuisine, weaving, Chinese medicine and ecological protection. However, no previous studies have focused on the endophytes of this plant. In...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-017-2267-9

    authors: Zhou YK,Shen XY,Hou CL

    更新日期:2017-06-01 00:00:00

  • Recent advances in constructing artificial microbial consortia for the production of medium-chain-length polyhydroxyalkanoates.

    abstract::Polyhydroxyalkanoates (PHAs) are a class of high-molecular-weight polyesters made from hydroxy fatty acid monomers. PHAs produced by microorganisms have diverse structures, variable physical properties, and good biodegradability. They exhibit similar physical properties to petroleum-based plastics but are much more en...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s11274-020-02986-0

    authors: Ai M,Zhu Y,Jia X

    更新日期:2021-01-04 00:00:00

  • Effect of pesticides on growth, respiration and nitrogenase activity ofAzotobacter andAzospirillum.

    abstract::Pesticides (Brominal, Cuprosan and Fenvalerate) at 10 and 50 ppm suppressed growth, respiration and nitrogenase activity ofAzotobacter chroococcum, Azospirillum brasilense andAzospirillum lipoferum. The inhibitory effect on respiration ofAsm. lipoferum was most pronounced after 3 and 4 days. ...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF01201891

    authors: Omar SA,Abd-Alla MH

    更新日期:1992-05-01 00:00:00

  • A synergism between oxalic acid and polygalacturonases in the depolymerization of potato tuber tissue.

    abstract::Rhizoctonia bataticola produced oxalic acid in vitro and in vivo during pathogenesis of patato tuber. Polygalacturonase (PG) was also detected in culture filtrates of the rot-causing organism. Levels of maceration and cell death in tuber tissue were higher when a mixture of oxalic acid and PG was used than when either...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00386304

    authors: Amadioha AC

    更新日期:1993-09-01 00:00:00

  • Temperature regulation of anaerobic degradation of organic matter.

    abstract::Anaerobic degradation of organic matter follows similar pathways in digesters and anaerobic freshwater sediments. The responsible microorganisms are linked in a complex food web, where short chain fatty acids and H2 are important intermediates. Degradation of short-chain fatty acids is endothermic under standard condi...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00419463

    authors: Westermann P

    更新日期:1996-09-01 00:00:00

  • Improvement of methanogenesis from cow dung and poultry litter waste digesters by addition of iron.

    abstract::When 50MM FeSO4 was added to cow dung and poultry litter waste which had been processed in daily-fed batch digesters, digesters subsequently unfed showed a faster conversion of substrate and overloaded digesters stabilized within 48 h. Early stabilization of digesters was achieved by adding 20 or 50MM FeSO4 though the...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00360890

    authors: Preeti Rao P,Seenayya G

    更新日期:1994-03-01 00:00:00

  • Evaluation of bacteria isolated from rice rhizosphere for biological control of charcoal rot of sorghum caused by Macrophomina phaseolina (Tassi) Goid.

    abstract::A total of 360 bacteria, isolated from the rhizospheres of a system of rice intensification (SRI) fields, were characterized for the production of siderophore, fluorescence, indole acetic acid (IAA), hydrocyanic acid (HCN) and solubilization of phosphorus. Of them, seven most promising isolates (SRI-156, -158, -178, -...

    journal_title:World journal of microbiology & biotechnology

    pub_type: 杂志文章

    doi:10.1007/s11274-010-0579-0

    authors: Gopalakrishnan S,Humayun P,Kiran BK,Kannan IG,Vidya MS,Deepthi K,Rupela O

    更新日期:2011-06-01 00:00:00