Activation of a calcium-dependent protein kinase involved in the Azospirillum growth promotion in rice.

Abstract:

:Rice seedlings (Oryza sativa) inoculated with the plant growth-promoting rhizobacteria Azospirillum brasilense FT326 showed an enhanced development of the root system 3 days after inoculation. Later on, a remarkable enlargement of shoots was also evident. An increase in the Ca2+-dependent histone kinase activity was also detected as a result of inoculation. The biochemical characterization and Western-blot analysis of the kinase strongly supports the hypothesis that it belongs to a member of the rice CDPK family. The fact that the amount of the protein did not change upon inoculation seems to indicate that a posttranslational activation is responsible for the change in the enzymatic activity. An in-gel kinase experiment identified a 46 kDa CDPK like protein kinase as a putative component of the signal transduction pathway triggered by Azospirillum inoculation. To our knowledge, this is the first report on the possible involvement of a Ca2+-dependent protein kinase in promotion of rice plants growth by A. brasilense.

authors

Ribaudo CM,Curá JA,Cantore ML

doi

10.1007/s11274-016-2186-1

subject

Has Abstract

pub_date

2017-02-01 00:00:00

pages

22

issue

2

eissn

0959-3993

issn

1573-0972

pii

10.1007/s11274-016-2186-1

journal_volume

33

pub_type

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