Abstract：
:Cyanobacterial 16S ribosomal RNA gene diversity was examined in a benthic mat on Fildes Peninsula of King George Island (62º09'54.4''S, 58º57'20.9''W), maritime Antarctica. Environmental DNA was isolated from the mat, a clone library of PCR-amplified 16S rRNA gene fragments was prepared, and amplified ribosomal DNA restriction analysis (ARDRA) was done to assign clones to seven groups. Low cyanobacterial diversity in the mat was suggested in that 83% of the clones were represented by one ARDRA group. DNA sequences from this group had high similarity with 16S rRNA genes of Tychonema bourrellyi and T. bornetii isolates, whose geographic origins were southern Norway and Northern Ireland. Cyanobacterial morphotypes corresponding to Tychonema have not been reported in Antarctica, however, this morphotype was previously found at Ward Hunt Lake (83ºN), and in western Europe (52ºN). DNA sequences of three of the ARDRA groups had highest similarity with 16S rDNA sequences of the Tychonema group accounting for 9.4% of the clones. Sequences of the remaining three groups (7.6%) had highest similarity with 16S rRNA genes of uncultured cyanobacteria clones from benthic mats of Lake Fryxell and fresh meltwater on the McMurdo Ice Shelf.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Callejas C,Gill PR,Catalán AI,Azziz G,Castro-Sowinski S,Batista Sdoi
10.1007/s11274-010-0578-1subject
Has Abstractpub_date
2011-06-01 00:00:00pages
1507-12issue
6eissn
0959-3993issn
1573-0972journal_volume
27pub_type
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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doi：10.1007/BF00419453
更新日期：1996-09-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00656517
更新日期：1993-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2012-02-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00656527
更新日期：1993-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
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更新日期：2017-05-01 00:00:00
