Abstract:
:Genome shuffling, an efficient and practical strain improvement technology via recursive protoplasts fusion, can break through the limits of species even genus to accelerate the directed evolution of microbial strains, without requiring the comprehensively cognized genetic background and operable genetic system. Hence this technology has been widely used for many important strains to obtain the desirable industrial phenotypes. In this review, we introduce the procedure of genome shuffling, discuss the new aid strategies of genome shuffling, summarize the applications of genome shuffling for increasing metabolite yield, improving strain tolerance, enhancing substrate utilization, and put forward the outlook to the future development of this technology.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Chen L,Xin QH,Ma LM,Li RF,Bian Kdoi
10.1007/s11274-020-02936-wsubject
Has Abstractpub_date
2020-09-24 00:00:00pages
158issue
10eissn
0959-3993issn
1573-0972pii
10.1007/s11274-020-02936-wjournal_volume
36pub_type
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