Characterization of a Rhizobium etli chromosomal gene required for nodule development on Phaseolus vulgaris L.

Abstract:

:A chromosomal gene, required for nodule development on Phaseolus bean, was characterized from Rhizobium etli strain TAL182. MLC640 is a Tn5 insertion mutant of TAL182 which shows decreased motility in soft TY agar and is defective in nodule development. The site of Tn5 insertion in MLC640 mapped to a 3.6-kb EcoRI chromosomal fragment. The 3.6-kb fragment was subcloned from the cosmid pUHR80 which complemented MLC640. Further subcloning and site-directed Tn5 mutagenesis localized the gene for nodule development to a 1.7-kb region within the 3.6-kb EcoRI fragment. Southern hybridization using the 3.6-kb EcoRI fragment as the probe against genomic DNA of several Rhizobium spp. indicated that this gene is conserved in different rhizobia.

authors

Pooyan S,George ML,Borthakur D

doi

10.1007/BF00367673

subject

Has Abstract

pub_date

1994-09-01 00:00:00

pages

583-9

issue

5

eissn

0959-3993

issn

1573-0972

journal_volume

10

pub_type

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