Abstract：
:Chymosin can specifically break down the Phe105-Met106 peptide bond of milk κ-casein to form insoluble para-κ-casein, resulting in milk coagulation, a process that is used in making cheese. In this study, in order to obtain an alternative milk coagulant which is safe and efficient, and simultaneously can produce cheese with a good taste, bovine prochymosin B was chosen and constitutively expressed to a high level in Pichia pastoris. The recombinant chymosin was expressed mainly as a secretory form, and it exhibited milk-clotting activity. It was purified by ammonium sulfate fractionation, anion exchange, followed by cation exchange chromatography. A final yield of 24.2% was obtained for the purified enzyme, which appeared as a single band in SDS-PAGE having a molecular mass of approximate 36 kDa. Proteolysis assay showed that it specifically hydrolyzed κ-casein. It was stable at 25-50°C and had optimal activity at 37°C and pH 4.0. The activity of the recombinant chymosin was activated by cations such as Mn(2+), Fe(3+), Mg(2+) and Na(+), but inhibited by K(+), Co(2+), Zn(2+), Ni(2+), and to a lesser extent by Cu(2+). These results suggested that recombinant bovine chymosin is an acid milk coagulant, and it could be considered as a safe and efficient enzyme suitable for use in cheese production.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Jiang XP,Yin ML,Chen P,Yang Qdoi
10.1007/s11274-012-1012-7subject
Has Abstractpub_date
2012-05-01 00:00:00pages
2087-93issue
5eissn
0959-3993issn
1573-0972journal_volume
28pub_type
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2013-10-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01198752
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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更新日期：1991-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2012-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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更新日期：2018-11-30 00:00:00
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更新日期：2020-02-22 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-014-1652-x
更新日期：2014-08-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF02310911
更新日期：1991-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF02421497
更新日期：1992-12-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1316-2
更新日期：2013-08-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1044-z
更新日期：2012-06-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-019-2693-y
更新日期：2019-07-22 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：1994-07-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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更新日期：2012-09-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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更新日期：2010-07-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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更新日期：2014-05-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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更新日期：2019-07-22 00:00:00
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journal_title：World journal of microbiology & biotechnology
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更新日期：2017-09-23 00:00:00
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2014-04-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2014-03-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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更新日期：2020-11-09 00:00:00
