Abstract：
:In Saccharomyces cerevisiae, los1 encodes a nuclear tRNA exporter. Despite the non-essentiality, the deletion of los1 has been shown to extend replicative life span in yeast. Here, we characterized AfuXpot, the los1 homologue in human pathogen Aspergillus fumigatus and found that it is continuously expressed during fungal growth. Microscopic examination of an AfuXpot-GFP-expressing transformant confirmed the nuclear localization of the fusion protein. The targeted gene deletion affirmed the non-essential role of AfuXpot in hyphal growth and sporulation. However, the growth of the deletion mutant was affected by amino acid, but not glucose, deprivation. The susceptibility of the deletant strain to protein and DNA/RNA synthesis inhibitors was also altered. Using bioinformatics tools, some transcription factor binding sites were predicted in AfuXpot promoter. Expression analyses of potential AfuXpot-interacting genes showed a marked down-regulation of sfp1 and mtr10 homologues in ΔAfuXpot strain. Our data demonstrates some conserved aspects of AfuXpot as a tRNA exporter in A. fumigatus.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Azizi A,SharifiRad A,Enayati S,Azizi M,Bayat M,Khalaj Vdoi
10.1007/s11274-020-2805-8subject
Has Abstractpub_date
2020-01-30 00:00:00pages
28issue
2eissn
0959-3993issn
1573-0972pii
10.1007/s11274-020-2805-8journal_volume
36pub_type
杂志文章abstract：:In a search for microorganisms producing extracellular protease with unhairing activity, Bacillus subtilis IIQDB32 was isolated. Protease formation was significantly stimulated by glucose, tryptone, yeast extract, Ca(2+) and Mn(2+), but was repressed by ammonia and Fe(2+). ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00327730
更新日期：1996-11-01 00:00:00
abstract：:Onion production in Argentina generates a significant amount of waste. Finding an effective method to recycle it is a matter of environmental concern. Among organic waste reuse techniques, anaerobic digestion could be a valuable alternative to current practices. Substrate inoculation with appropriate bacterial strains...
journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-018-2465-0
更新日期：2018-05-30 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1062-x
更新日期：2012-07-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF02310921
更新日期：1991-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2012-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00327954
更新日期：1994-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-016-2087-3
更新日期：2016-08-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF01238789
更新日期：1992-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00360880
更新日期：1994-03-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00364617
更新日期：1995-07-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s11274-018-2491-y
更新日期：2018-07-04 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00656517
更新日期：1993-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1311-7
更新日期：2013-08-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00361025
更新日期：1995-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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doi：10.1007/s11274-017-2267-9
更新日期：2017-06-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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更新日期：2013-10-01 00:00:00
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更新日期：2013-05-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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更新日期：1993-05-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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更新日期：2014-04-01 00:00:00
abstract：:Shewanella colwelliana, a marine bacterium isolated in association with the oyster Crassostrea virginica, produces an abundant exopolysaccharide with potential commercial value as an adhesive under aqueous conditions. Its utilization of glucose was modulated by stoichiometric concentrations of yeast extract. In Brain ...
journal_title：World journal of microbiology & biotechnology
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更新日期：1994-09-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：1990-12-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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更新日期：2012-02-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2014-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：1996-05-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1179-y
更新日期：2013-02-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
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更新日期：2018-11-30 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-005-9028-x
更新日期：2006-03-01 00:00:00
