Cryopreservation of yeasts in polypropylene straws.

abstract：:The cloning and expression of toxic proteins in bacteria have posed a great challenge because of the leaky expression in inducible expression systems. Using artificial gene synthesis and clone screening methods, we identified a mutant T5 promoter, which significantly reduced leaky expression of lac operator. The mutan...

journal_title：World journal of microbiology & biotechnology

authors： Guo J,Jia R

更新日期：2014-05-01 00:00:00

abstract：:A locally isolated Acinetobacter sp. Strain AQ5NOL 1 was encapsulated in gellan gum and its ability to degrade phenol was compared with the free cells. Optimal phenol degradation was achieved at gellan gum concentration of 0.75% (w/v), bead size of 3 mm diameter (estimated surface area of 28.26 mm(2)) and bead number ...

journal_title：World journal of microbiology & biotechnology

authors： Ahmad SA,Shamaan NA,Arif NM,Koon GB,Shukor MY,Syed MA

更新日期：2012-01-01 00:00:00

abstract：:The objectives of this study were firstly, to determine the genetic diversity of Monilinia laxa isolates from Hungary, using the PCR-based inter-simple sequence repeat (ISSR) and randomly amplified polymorphic DNA (RAPD) technique; secondly, to prepare genetic diversity groups based on the dendrograms; and finally, to...

journal_title：World journal of microbiology & biotechnology

authors： Fazekas M,Madar A,Sipiczki M,Miklós I,Holb IJ

更新日期：2014-06-01 00:00:00

abstract：:With rapid urbanization, anthropogenic activities are increasingly influencing the natural environment of the Bohai Bay. In this study, the composition and variation of bacterial and nirS-harboring bacterial communities in the coastal zone sediments of the Bohai Gulf were analyzed using PCR-based clone libraries. A to...

journal_title：World journal of microbiology & biotechnology

authors： Guan X,Zhu L,Li Y,Xie Y,Zhao M,Luo X

更新日期：2014-04-01 00:00:00

abstract：:Solid-state fermentation (SSF) with Agaricus brasiliensis and Agaricus bisporus on corn was carried out. The results showed that SSF with the two fungi made up the deficiency of tryptophan in corn and improved the protein nutritional value of corn. The conjugated polyphenols contents in fermented corn decreased and fr...

journal_title：World journal of microbiology & biotechnology

authors： Zhai FH,Liu HY,Han JR

更新日期：2018-02-15 00:00:00

abstract：:Studies were carried out to screen and identify strains that are able to directly produce ferulic oligosaccharides (FOs) from wheat bran (WB). The inducement and distribution of hemicellulases from strain 2012, which was identified as a non-melanin secreting strain of Aureobasidium pullulans (A. pullulans), were also ...

journal_title：World journal of microbiology & biotechnology

authors： Yu XH,Gu ZX

更新日期：2014-02-01 00:00:00

abstract：:When various lignin-related para-phenolic benzoic acids, para-phenolic cinnamic acids, para-phenolic phenylpropionic acids, the corresponding unsubstituted and 4-O-methylated derivatives, and 4-hydroxyl substituted benzaldehydes were tested on the growth of eight white-rot fungi, methylation of the 4-hydroxy substitue...

journal_title：World journal of microbiology & biotechnology

authors： Buswell JA,Eriksson KE

更新日期：1994-03-01 00:00:00

abstract：:An efficient expression-secretion system for heterologous protein production in food-grade hosts, Lactobacillus plantarum and Bacillus subtilis, is still required to broaden their applications. The optimal signal peptide compatible with both the desired protein and the target host is important for the system. Here, we...

journal_title：World journal of microbiology & biotechnology

authors： Promchai R,Visessanguan W,Luxananil P

更新日期：2020-09-19 00:00:00

abstract：:Filamentous fungi, isolated from the enriched surface of garden soil, were screened for α-galactosidase production after growing on wheat-bran/carbohydrate substrate. One isolate,Aspergillus niger, had the highest enzyme activity (5.1×10(-2) units/mg protein) at pH 5.0 and 50°C. Treatment of cowpea flour with the crud...

journal_title：World journal of microbiology & biotechnology

authors： Somiari RI,Balogh E

更新日期：1992-11-01 00:00:00

abstract：:Quaternary ammonium salts (QASs) are ubiquitous in nature, being found in organisms ranging from microorganisms to vertebrates (e.g., glycine betaine, carnitine) where they have important cellular functions. QASs are also obtained by chemical synthesis. These compounds, due to their diverse chemical structure (e.g. mo...

journal_title：World journal of microbiology & biotechnology

authors： Obłąk E,Futoma-Kołoch B,Wieczyńska A

更新日期：2021-01-11 00:00:00

abstract：:A commercial cellulase was first assessed to be effective in hydrolyzing glycosyl at the C-3 and C-26 positions in steroidal saponins from yellow ginger (Dioscorea zingiberensis C. H. Wright) to diosgenin, a very important chemical in the pharmaceutical industry. The effect of different parameters on enzyme hydrolysis...

journal_title：World journal of microbiology & biotechnology

authors： Liu W,Huang W,Sun W,Zhu Y,Ni J

更新日期：2010-07-01 00:00:00

abstract：:Opuntia ficus-indica Mill. (forage cactus) is farmed with relative success in the semi-arid region of the Brazilian northeast for commercial purposes, particularly as forage and food. Endophytic microorganisms are those that can be isolated inside plant tissues and can be a new source to production of enzymes with dif...

journal_title：World journal of microbiology & biotechnology

authors： Bezerra JD,Santos MG,Svedese VM,Lima DM,Fernandes MJ,Paiva LM,Souza-Motta CM

更新日期：2012-05-01 00:00:00

abstract：:In surface cultures of Streptomyces cyaneus var. viridochromogenes, NaCl depressed water activity (a w) without supporting growth. Reducing a w from 0.987 to 0.951 led to 3- and 4-fold increases in intracellular and extracellular phenol oxidase activities, respectively. ...

journal_title：World journal of microbiology & biotechnology

authors： Berrocal M,Huerta S,Rodríguez J,Pérez-Leblic M,Arias ME

更新日期：1996-01-01 00:00:00

abstract：:Yeasts and yeast-like organisms were chosen for the aerobic treatment of cassava starch factory effluent. A mixed culture of Candida utills and Endomycopsis fibuliger efficiently and rapidly utilized both starch and free sugars. After 28 h fermentation the protein content of the biomass was 22% (w/w), which remained u...

journal_title：World journal of microbiology & biotechnology

authors： Manilal VB,Narayanan CS,Balagopalan C

更新日期：1991-03-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris is widely used in recombinant expression of eukaryotic proteins owing to the ability of post-translational modification, tightly regulated promoters, and high cell density fermentation. However, episomal plasmids for heterologous gene expression and the CRISPR/Cas9 system for g...

journal_title：World journal of microbiology & biotechnology

authors： Gu Y,Gao J,Cao M,Dong C,Lian J,Huang L,Cai J,Xu Z

更新日期：2019-05-27 00:00:00

abstract：:We characterised the anti-Vibrio parahaemolyticus (anti-V. parahaemolyticus) marine bacteria DIT09, DIT44 and DIT46 isolated from the intertidal mussel Perumytilus purpuratus. The 16S rRNA gene sequences identify a Pseudoalteromonas sp. that form a clade with P. prydzensis and P. mariniglutinosa. The strains produced ...

journal_title：World journal of microbiology & biotechnology

authors： Aranda CP,Valenzuela C,Barrientos J,Paredes J,Leal P,Maldonado M,Godoy FA,Osorio CG

更新日期：2012-06-01 00:00:00

abstract：:Rice bran was superior to other proteinaceous substrates for protease production by Rhizopus oligosporus ACM 145F in solid-state fermentation. Maximum protease yield was after 72 h. The optimal initial moisture content was 47% (a w=0.97). Dried, ground and resuspended fermented rice was the most pratical and effective...

journal_title：World journal of microbiology & biotechnology

authors： Ikasari L,Mitchell DA

更新日期：1994-05-01 00:00:00

abstract：:Ginger (Zingiber officinale) is cultivated commercially in most parts of the world especially in India for its culinary and medicinal applications. One of the major challenges that limit the yield of ginger is rhizome rot disease caused by organisms including Pythium myriotylum. A feasible ecofriendly method is yet to...

journal_title：World journal of microbiology & biotechnology

authors： Jasim B,Anisha C,Rohini S,Kurian JM,Jyothis M,Radhakrishnan EK

更新日期：2014-05-01 00:00:00

abstract：:A key constraint in successfully obtaining an effective inoculant is overcoming difficulties in formulating a viable and user-friendly final product and maintaining the microbial cells in a competent state. Co-cultures of rhizobia and PGPR (Plant Growth Promoting Rhizobacteria) are a logical next subject for formulati...

journal_title：World journal of microbiology & biotechnology

authors： Atieno M,Herrmann L,Okalebo R,Lesueur D

更新日期：2012-07-01 00:00:00

abstract：:Heavy-metal chromium [Cr(VI)] is a ubiquitous environmental pollutant. Comparing with chemical reduction, microbiological reduction is considered to be a friendly and cheaper way to decrease the damage caused by chromate. A bacterial strain, CR-07, which is resistant to and capable of reducing chromate was isolated fr...

journal_title：World journal of microbiology & biotechnology

authors： Liu Z,Wu Y,Lei C,Liu P,Gao M

更新日期：2012-04-01 00:00:00

abstract：:Gene regulation studies in pseudomonad bacteria are mainly restricted to Pseudomonas aeruginosa and Pseudomonas putida. Constitutive promoters exhibit DNA sequences similar to the σ (70)-dependent constitutive promoters of Escherichia coli. The TOL meta-cleavage pathway operon promoter and the nah operon promoters are...

journal_title：World journal of microbiology & biotechnology

authors： Ramos JL,Marqués S

更新日期：1993-07-01 00:00:00

abstract：:Common reed (Phragmites australis) and narrow-leaved cattail (Typha angustifolia L.) are two plant species used widely in artificial wetlands constructed to treat wastewater. In this study, the community structure and diversity of root-associated bacteria of common reed and narrow-leaved cattail growing in the Beijing...

journal_title：World journal of microbiology & biotechnology

authors： Li YH,Zhu JN,Liu QF,Liu Y,Liu M,Liu L,Zhang Q

更新日期：2013-08-01 00:00:00

abstract：:Paclitaxel is a highly toxic anticancer agent which is used in a wide range against ovarian, breast, lung, and prostate cancers. Paclitaxel is manufactured recently in the north of Iran which may lead to the introduction of the drug into the environment via pharmaceutical wastewater. To our knowledge, Paclitaxel degra...

journal_title：World journal of microbiology & biotechnology

authors： Zamani H,Grakoee SR,Rakhshaee R

更新日期：2016-08-01 00:00:00

abstract：:The microbial community of artisanal corn fermentation called Chicha were isolated, purified and then identified using protein profile by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) and confirmed by partial ribosomal gene sequencing. Samples from Chicha beverage were chemic...

journal_title：World journal of microbiology & biotechnology

authors： Resende LV,Pinheiro LK,Miguel MGDCP,Ramos CL,Vilela DM,Schwan RF

更新日期：2018-03-08 00:00:00

abstract：:The effects of different alkyl chain lengths of ionic liquids 1-ethyl-, 1-butyl- and 1-hexyl-3-methylimidazolium chloride, on the catalytic activity, thermal stability and deactivation kinetics of horseradish peroxidase were studied in the temperature range of 45-85 °C. The presence of 1-ethyl- and 1-butyl-ionic liqui...

journal_title：World journal of microbiology & biotechnology

authors： Machado MF,Queirós RP,Santos MD,Fidalgo LG,Delgadillo I,Saraiva JA

更新日期：2014-02-01 00:00:00

abstract：:Nitrification plays a significant role in the global nitrogen cycle, and this concept has been challenged with the discovery of ammonia-oxidizing archaea (AOA) in the environment. In this paper, the vertical variations of the diversity and abundance of AOA in the hyporheic zone of the Fuyang River in North China were ...

journal_title：World journal of microbiology & biotechnology

authors： Wang Z,Wang Z,Huang C,Pei Y

更新日期：2014-04-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHAs) are a class of high-molecular-weight polyesters made from hydroxy fatty acid monomers. PHAs produced by microorganisms have diverse structures, variable physical properties, and good biodegradability. They exhibit similar physical properties to petroleum-based plastics but are much more en...

journal_title：World journal of microbiology & biotechnology

authors： Ai M,Zhu Y,Jia X

更新日期：2021-01-04 00:00:00

abstract：:A superoxide dismutase (SOD) was purified from Spirulina platensis sonicate. The SOD was purified to homogeneity (48-fold and 0.24% yield) through ammonium sulphate precipitation and DEAE-52 anion exchange chromatography. The SOD from S. platensis appeared to be a homodimer with a molecular weight of 30 kDa and a subu...

journal_title：World journal of microbiology & biotechnology

authors： Desai K,Sivakami S

更新日期：2007-12-01 00:00:00

abstract：:5-Enopyruvylshikimate-3-phosphate synthase (EPSP synthase) is an important enzyme in the shikimate pathway mediating the biosynthesis of aromatic compounds in plants and microorganisms. A novel class II EPSP synthase AroA S. fredii from Sinorhizobium fredii NGR234 was overexpressed in Escherichia coli BL21. It was pur...

journal_title：World journal of microbiology & biotechnology

authors： Wang L,Peng R,Tian Y,Han J,Zhao W,Wang B,Liu M,Yao Q

更新日期：2014-11-01 00:00:00

abstract：:Acetic acid is an inhibitor in industrial processes such as wine making and bioethanol production from cellulosic hydrolysate. It causes energy depletion, inhibition of metabolic enzyme activity, growth arrest and ethanol productivity losses in Saccharomyces cerevisiae. Therefore, understanding the mechanisms of the y...

journal_title：World journal of microbiology & biotechnology

authors： Geng P,Zhang L,Shi GY

更新日期：2017-05-01 00:00:00