Abstract:
:Opuntia ficus-indica Mill. (forage cactus) is farmed with relative success in the semi-arid region of the Brazilian northeast for commercial purposes, particularly as forage and food. Endophytic microorganisms are those that can be isolated inside plant tissues and can be a new source to production of enzymes with different potentialities. The objective of this study was to describe the richness of endophytic fungi from O. ficus-indica and to detect the capacity of these species to produce extracellular hydrolytic enzymes. Forty-four endophytic fungi species were isolated. Among them, the most commonly found were Cladosporium cladosporioides (20.43%) and C. sphaerospermum (15.99%). Acremonium terricola, Monodictys castaneae, Penicillium glandicola, Phoma tropica and Tetraploa aristata are being reported for the first time as endophytic fungi for Brazil. The majority of isolated fungi exhibited enzymatic potential. Aspergillus japonicus and P. glandicola presented pectinolytic activity. Xylaria sp. was the most important among the other 14 species with positive cellulase activity. All 24 isolates analysed were xylanase-positive. Protease was best produced by isolate PF103. The results indicate that there is a significant richness of endophytic fungi in O. ficus-indica, and that these isolates indicate promising potential for deployment in biotechnological processes involving production of pectinases, cellulases, xylanases and proteases.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Bezerra JD,Santos MG,Svedese VM,Lima DM,Fernandes MJ,Paiva LM,Souza-Motta CMdoi
10.1007/s11274-011-1001-2subject
Has Abstractpub_date
2012-05-01 00:00:00pages
1989-95issue
5eissn
0959-3993issn
1573-0972journal_volume
28pub_type
杂志文章abstract::Mangrove fungi are vastly unexplored for enzymes with industrial application. This study aimed to assess the biocatalytic activity of mangrove fungal xylanases on recycled paper pulp. Forty-four mangrove fungal (MF) isolates were initially screened for xylanolytic activity in minimal medium with corn cob xylan as the ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-012-1220-1
更新日期:2013-04-01 00:00:00
abstract::Escherichia coli (E. coli) consists of commensal (ComEC) and diarrhoeagenic (DEC) groups. ComEC are detected using traditional culture methods. Conformational steps are performed after culturing if it is required to test for the presence of DEC, increasing cost and time in obtaining the results. The aim of this study ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1690-4
更新日期:2014-10-01 00:00:00
abstract::When cell membranes of Lentinus edodes mycelium were rapidly frozen at either 50 or 160°C/min, viability was lost and this correlated with rupture of the plasmalemma and residual membrane material and with alterations in the organelles. Although with slow cooling (1°C/min) 80% of the samples recovered viability, some ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00369571
更新日期:1993-11-01 00:00:00
abstract::Over the past years, short anti-microbial peptides have drawn growing attention in the research and trade literature because they are usually capable of killing a broad spectrum of pathogens by employing unique mechanisms of action. This study aimed to evaluate the anti-bacterial effects of a previously designed pepti...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-020-02948-6
更新日期:2020-10-21 00:00:00
abstract::Nitrification plays a significant role in the global nitrogen cycle, and this concept has been challenged with the discovery of ammonia-oxidizing archaea (AOA) in the environment. In this paper, the vertical variations of the diversity and abundance of AOA in the hyporheic zone of the Fuyang River in North China were ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1559-y
更新日期:2014-04-01 00:00:00
abstract::Trichoderma asperellum parasitizes a large variety of phytopathogenic fungi. The mycoparasitic activity of T. asperellum depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall and proteases which are a group of enzymes capable of degrading proteins from host. In this stud...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1373-6
更新日期:2013-11-01 00:00:00
abstract::The methylotrophic yeast Pichia pastoris is widely used in recombinant expression of eukaryotic proteins owing to the ability of post-translational modification, tightly regulated promoters, and high cell density fermentation. However, episomal plasmids for heterologous gene expression and the CRISPR/Cas9 system for g...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2654-5
更新日期:2019-05-27 00:00:00
abstract::A chromosomal gene, required for nodule development on Phaseolus bean, was characterized from Rhizobium etli strain TAL182. MLC640 is a Tn5 insertion mutant of TAL182 which shows decreased motility in soft TY agar and is defective in nodule development. The site of Tn5 insertion in MLC640 mapped to a 3.6-kb EcoRI chro...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00367673
更新日期:1994-09-01 00:00:00
abstract::A total of 360 bacteria, isolated from the rhizospheres of a system of rice intensification (SRI) fields, were characterized for the production of siderophore, fluorescence, indole acetic acid (IAA), hydrocyanic acid (HCN) and solubilization of phosphorus. Of them, seven most promising isolates (SRI-156, -158, -178, -...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-010-0579-0
更新日期:2011-06-01 00:00:00
abstract::Fungal morphogenesis is a regulated series of events, leading to changes from one state to another, in which proteolysis could be regarded as one of the controlling functions. Proteinases are essential for the supply of amino acids, selective inactivation of specific growth phase proteins not required during developme...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF01201871
更新日期:1992-05-01 00:00:00
abstract::One strain of algicidal bacterium, named as S088, was isolated from the intestine of healthy sea cucumbers (Stichopus horrens) in the South China Sea. Based on the analysis of its biochemical characteristics and 16S rDNA gene sequence, S088 was identified as Bowmanella denitrificans. Importantly, the algicidal activit...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1478-y
更新日期:2014-02-01 00:00:00
abstract::Tomato (Lycopersicon esculentum) is important widely grown vegetable in India and its productivity is affected by bacterial wilt disease infection caused by Ralstonia solanacearum. To prevent this disease infection a study was conducted to isolate and screen effective plant growth promoting rhizobacteria (PGPR) antago...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-011-0975-0
更新日期:2012-04-01 00:00:00
abstract::The mushroom Flammulina velutipes and the white-rot fungus Trametes versicolor were cultivated separately on sugarcane bagasse for 40 days. Trametes versicolor produced laccase and manganese-peroxidase activities, showing a simultaneous degradation of lignin and holocellulose. However, only phenoloxidase activity was ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00286370
更新日期:1995-09-01 00:00:00
abstract::This study was conducted to report the richness of endophytic Penicillium and Talaromyces species isolated from Tillandsia catimbauensis, a bromeliad endemic in the Brazilian tropical dry forest (Caatinga), to verify their ability to produce the enzyme L-asparaginase and to partially optimise the production of biomass...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-018-2547-z
更新日期:2018-10-28 00:00:00
abstract::Biomineralization is a process by which living organisms produce minerals. The extracellular production of these biominerals by microbes has potential for various bioengineering applications. For example, crack remediation and improvement of durability of concrete is an important goal for engineers and biomineral-prod...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-015-1948-5
更新日期:2015-12-01 00:00:00
abstract::The ideal vaccine is discussed under three headings. 1. The major requirements of the vaccine. This includes primarlly safety and efficacy and a number of other desirable features if the vaccine is to control a disease of global importance. These include cost, easy administration (e.g. orally), thermal stability, mult...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00328978
更新日期:1991-03-01 00:00:00
abstract::The biomass degrading enzymatic potential of 101 thermophilic bacterial strains isolated from a volcanic environment (Santorini, Aegean Sea, Greece) was assessed. 80 % of the strains showed xylanolytic activity in Congo Red plates, while only eight could simultaneously hydrolyze cellulose. Fifteen isolates were select...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-012-1100-8
更新日期:2012-09-01 00:00:00
abstract::Activity of α-L-arabinofuranosidae (EC 3.2.1.55) ranged between 0.03 and 1.63 U/ml whenSclerotium rolfsii was grown in a synthetic medium containing different nitrogen and carbon sources. Agricultural and forest residues such as rice straw, corn cob, groundnut husk, and apple pomace were suitable substrates for enzyme...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00704656
更新日期:1995-03-01 00:00:00
abstract::Lactococcus lactis subsp. lactis CRL 1584 isolated from a bullfrog hatchery produces a bacteriocin that inhibits both indigenous Citrobacter freundii (a Red-Leg Syndrome related pathogen) and Lactobacillus plantarum, and Listeria monocytogenes as well. Considering that probiotics requires high cell densities and/or ba...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-017-2353-z
更新日期:2017-09-27 00:00:00
abstract::Cobamides ('complete' corrinoids) are essential for organohalide-respiring bacteria because they act as cofactors of reductive dehalogenases (RDases). RDases are the key enzymes in organohalide respiration, a process relevant for environmental remediation. More than a decade ago, the unusual norpseudo-B12 was identifi...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章,评审
doi:10.1007/s11274-017-2258-x
更新日期:2017-05-01 00:00:00
abstract::Purified cell walls from Bacillus subtilis were repeatedly suspended in 5 mM CuCl2 and, after removing unbound Cu, were suspended in 1% (v/v) HNO3 to release bound Cu. The walls were then regenerated by washing in H2O. After five cycles, copper binding actually increased slightly, probably due to enhanced exposure of ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00144475
更新日期:1994-07-01 00:00:00
abstract::This study investigated effects of wet and dry seasons on the bacterial community structure of the Three Gorges Reservoir by using denaturing gradient gel electrophoresis analysis of the PCR-amplified bacterial 16S rRNA gene. Bacterial diversity, as determined by the Shannon index, the Simpson's index, and the Richnes...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-012-1239-3
更新日期:2013-05-01 00:00:00
abstract::Catechol 2,3-oxygenase was produced by Escherichia coli, harbouring the recombinant plasmid pBH100 which contained the pheB gene cloned from phenol-degrading Pseudomonas putida BH, and was applied for the determination of catechol in the liquor. E. coli JM103 (pBH100) and C600 (pBH100) showed, respectively, about 5 an...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00329409
更新日期:1991-05-01 00:00:00
abstract::Prebiotic Fructooligosaccharides (FOS) escape metabolism in upper GI tract undergo microbial metabolism in colon and thereby influence the nature, type and number of intestinal microbiota to improve host's health. The present study focuses on the ability of Lactobacillus plantarum CFR 2194 to utilize FOS as a selectiv...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-011-0887-z
更新日期:2012-03-01 00:00:00
abstract::The effects of different alkyl chain lengths of ionic liquids 1-ethyl-, 1-butyl- and 1-hexyl-3-methylimidazolium chloride, on the catalytic activity, thermal stability and deactivation kinetics of horseradish peroxidase were studied in the temperature range of 45-85 °C. The presence of 1-ethyl- and 1-butyl-ionic liqui...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-013-1466-2
更新日期:2014-02-01 00:00:00
abstract::The rare actinomycetes strain 2EPS was isolated from soil and analysis of cultural, morphological characteristics, diaminopimelic acid content of its cell wall, and 16S rRNA gene sequence indicates that 2EPS belongs to genus Actinomadura. In addition, neighbor-joining phylogenetic tree also confirmed the relationships...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-014-1792-z
更新日期:2015-02-01 00:00:00
abstract::The new transcription factor Sge1 has garnered much attention in filamentous fungi recently, which highlights its role in pathogenicity, conidiation, and the production of secondary metabolites. In this study, we demonstrated that FgSge1 is localized in the nucleus in Fusarium graminearum using fluorescent protein GFP...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-015-1894-2
更新日期:2015-09-01 00:00:00
abstract::An efficient expression-secretion system for heterologous protein production in food-grade hosts, Lactobacillus plantarum and Bacillus subtilis, is still required to broaden their applications. The optimal signal peptide compatible with both the desired protein and the target host is important for the system. Here, we...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-020-02932-0
更新日期:2020-09-19 00:00:00
abstract::Penicillium citrinum, using rice husks in a solid state fermentation, produced maximum cellulase yields (37 Units/g) after 12 days with a cellulose utilization of more than 70%. Enzyme yields were three times higher than in shake-flask cultures. ...
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/BF00656527
更新日期:1993-01-01 00:00:00
abstract::This paper aims to characterize halophilic bacteria inhabiting Algerian Saline Ecosystems (Sebkha and Chott) located in arid and semi-arid ecoclimate zones (Northeastern Algeria). In addition, screening of enzymatic activities, heavy metal tolerance and antagonistic potential against phytopathogenic fungi were tested....
journal_title:World journal of microbiology & biotechnology
pub_type: 杂志文章
doi:10.1007/s11274-019-2705-y
更新日期:2019-08-20 00:00:00