Abstract：
:The rare actinomycetes strain 2EPS was isolated from soil and analysis of cultural, morphological characteristics, diaminopimelic acid content of its cell wall, and 16S rRNA gene sequence indicates that 2EPS belongs to genus Actinomadura. In addition, neighbor-joining phylogenetic tree also confirmed the relationships of this strain to other members of Actinomadura. A butanol extract with antibacterial activity was purified by reversed-phase chromatography to obtain three bioactive compounds, designated as compounds 1, 2 and 3. The structures of these compounds were determined using spectroscopic analysis ((1)H-NMR and (13)C-NMR) and mass spectrometric analysis (HR-TOF-MS). Compounds 1-3 were identified and found to be the same as those included in the Japanese patent number JP 09227587 for spirotetronate antibiotics and are BE-45722A (1), BE-45722B (2) and BE-45722C (3), respectively. All compounds were active against Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 14579, and B. subtilis ATCC 6633) with low MIC values between 0.08 and 5.0 µg/ml. Moreover, both 1 and 3 also exhibited strong activity, with similar MIC values, against Clostridium perfringens S107 at 0.63 µg/ml and C. difficile 630 at 0.08 µg/ml. These results suggest the identified spirotetronate compounds may have potential in the treatment of Clostridium infections. Overall, this analysis demonstrates that rare actinomycetes are a promising source for discovery of antimicrobial compounds.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Euanorasetr J,Intra B,Mongkol P,Chankhamhaengdecha S,Tuchinda P,Mori M,Shiomi K,Nihira T,Panbangred Wdoi
10.1007/s11274-014-1792-zsubject
Has Abstractpub_date
2015-02-01 00:00:00pages
391-8issue
2eissn
0959-3993issn
1573-0972journal_volume
31pub_type
杂志文章abstract：:Anaerobic degradation of organic matter follows similar pathways in digesters and anaerobic freshwater sediments. The responsible microorganisms are linked in a complex food web, where short chain fatty acids and H2 are important intermediates. Degradation of short-chain fatty acids is endothermic under standard condi...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00419463
更新日期：1996-09-01 00:00:00
abstract：:Yeasts and yeast-like organisms were chosen for the aerobic treatment of cassava starch factory effluent. A mixed culture of Candida utills and Endomycopsis fibuliger efficiently and rapidly utilized both starch and free sugars. After 28 h fermentation the protein content of the biomass was 22% (w/w), which remained u...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00328988
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00656517
更新日期：1993-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-015-2001-4
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00327730
更新日期：1996-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-016-2178-1
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00360920
更新日期：1996-05-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1179-y
更新日期：2013-02-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-019-2762-2
更新日期：2019-11-14 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00360930
更新日期：1996-05-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00364677
更新日期：1996-03-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00361025
更新日期：1995-11-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1012-7
更新日期：2012-05-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00327812
更新日期：1996-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1432-z
更新日期：2014-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-010-0578-1
更新日期：2011-06-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-012-1023-4
更新日期：2012-05-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2014-07-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
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更新日期：2019-11-14 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-013-1478-y
更新日期：2014-02-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章,评审
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更新日期：2021-01-11 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-017-2399-y
更新日期：2018-02-15 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/BF00340211
更新日期：1996-07-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-011-0846-8
更新日期：2012-02-01 00:00:00
abstract：:The search for new microbial strains that are able to withstand inhibitors released from hemicellulosic hydrolysis and are also still able to convert sugars in ethanol/xylitol is highly desirable. A yeast strain isolated from sugarcane juice and identified as Meyerozyma guilliermondii was evaluated for the ability to ...
journal_title：World journal of microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s11274-016-2036-1
更新日期：2016-05-01 00:00:00
