Abstract：
:Catechol 2,3-oxygenase was produced by Escherichia coli, harbouring the recombinant plasmid pBH100 which contained the pheB gene cloned from phenol-degrading Pseudomonas putida BH, and was applied for the determination of catechol in the liquor. E. coli JM103 (pBH100) and C600 (pBH100) showed, respectively, about 5 and 8.5 times higher activities than that of P. putida BH. Using the crude extract prepared from the culture broth of the recombinant, catechol between 0.1 and 3.0 μg/ml could be determined quantitatively in phosphate buffer, synthetic sewage and in mixtures of phenol, benzoate and sallcylate, and also in sodium pyruvate solution. In addition to catechol, 3-methylcatechol, 4-methylcatechol and 4-chlorocatechol could be determined. Oxygenase activity of the crude extract was maintained completely during the 100-day storage at -20°C after being freeze-dried with 10% acelone.
journal_name
World J Microbiol Biotechnoljournal_title
World journal of microbiology & biotechnologyauthors
Fujita M,Kamiya T,Ike M,Kawagoshi Y,Shinohara Ndoi
10.1007/BF00329409subject
Has Abstractpub_date
1991-05-01 00:00:00pages
407-14issue
3eissn
0959-3993issn
1573-0972journal_volume
7pub_type
杂志文章abstract：:Genome shuffling, an efficient and practical strain improvement technology via recursive protoplasts fusion, can break through the limits of species even genus to accelerate the directed evolution of microbial strains, without requiring the comprehensively cognized genetic background and operable genetic system. Hence...
journal_title：World journal of microbiology & biotechnology
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更新日期：1996-01-01 00:00:00
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journal_title：World journal of microbiology & biotechnology
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更新日期：1996-01-01 00:00:00
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